Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,595,335	T→C	A175A (GCA→GCG)	tmcA ←	elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,595,335	0	T	C	100.0%	182.2 / NA	50	A175A (GCA→GCG)	tmcA	elongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/19);  total (31/19)

GCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTC  >  NC_000913/2595187‑2595478                                                                                                                                                     |                                                                                                                                                gcgcGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGgcgc                                                                                                                                               <  1:44719/151‑1 (MQ=255)        gACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGt                                                                                                                                         <  1:536124/150‑1 (MQ=255)         aCAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTcg                                                                                                                                       <  2:294863/151‑1 (MQ=255)                   tGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTAcc                                                                                                                              >  2:621843/1‑150 (MQ=255)                    gCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTAcc                                                                                                                              >  2:355279/1‑149 (MQ=255)                    gCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACca                                                                                                                             <  1:118948/150‑1 (MQ=255)                     cccGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACcagt                                                                                                                           >  1:51519/1‑151 (MQ=255)                     cccGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACcagt                                                                                                                           <  2:43373/151‑1 (MQ=255)                        gCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACcagtcag                                                                                                                        <  2:360864/151‑1 (MQ=255)                          aaTACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACcagtcagt                                                                                                                       >  1:310810/1‑150 (MQ=255)                            tACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACg                                                                                                                    >  1:18807/1‑151 (MQ=255)                              cGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACggg                                                                                                                  >  1:574208/1‑151 (MQ=255)                               gagaAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACgggg                                                                                                                 >  1:292296/1‑151 (MQ=255)                               gagaAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACggg                                                                                                                  >  2:297932/1‑150 (MQ=255)                                agaAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGa                                                                                                                <  2:32348/151‑1 (MQ=255)                                   aaTGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGt                                                                                                              >  1:665058/1‑150 (MQ=255)                                    aTGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTa                                                                                                             >  2:48091/1‑150 (MQ=255)                                      gAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTaaaa                                                                                                          <  2:524149/151‑1 (MQ=255)                                          tGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATgcg                                                                                                      >  2:274775/1‑151 (MQ=255)                                              cTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAGAATGCGcc                                                                                                    >  2:456514/1‑149 (MQ=255)                                                  cAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCTCCAACgag                                                                                              <  2:93431/151‑1 (MQ=255)                                                     cGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGaa                                                                                            >  1:248681/1‑150 (MQ=255)                                                         gACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCt                                                                                       >  1:109528/1‑151 (MQ=255)                                                         gACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCt                                                                                       >  1:526629/1‑151 (MQ=255)                                                             tACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTgg                                                                                     <  2:646382/149‑1 (MQ=255)                                                             tACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGtt                                                                                   >  1:421204/1‑151 (MQ=255)                                                               ccGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGtttt                                                                                 <  1:570446/151‑1 (MQ=255)                                                                cgcgCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGtttt                                                                                 >  2:393443/1‑150 (MQ=255)                                                                       aCGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTgccgcc                                                                           >  2:528263/1‑149 (MQ=255)                                                                           gcAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAgg                                                                      >  2:625146/1‑150 (MQ=255)                                                                                 gTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCt                                                               >  2:405944/1‑151 (MQ=255)                                                                                       gCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTAt                                                         <  2:148840/151‑1 (MQ=255)                                                                                       gCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTAt                                                         <  2:433244/151‑1 (MQ=255)                                                                                          gCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATccg                                                      <  2:131526/151‑1 (MQ=255)                                                                                          gCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATcc                                                       <  1:467017/150‑1 (MQ=255)                                                                                                 ccGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTa                                                 >  1:570809/1‑149 (MQ=255)                                                                                                          tGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGttt                                      >  2:210587/1‑151 (MQ=255)                                                                                                           ggTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTg                                     >  1:230540/1‑151 (MQ=255)                                                                                                              cATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTg                                     >  1:39682/1‑148 (MQ=255)                                                                                                                 tAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGc                               >  2:196155/1‑151 (MQ=255)                                                                                                                  aGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCt                              >  1:315053/1‑151 (MQ=255)                                                                                                                      gctTTAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGAc                          >  2:164151/1‑151 (MQ=255)                                                                                                                          tAAGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACaaa                       <  1:391734/150‑1 (MQ=255)                                                                                                                           aaGAGTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAAt                     >  2:248454/1‑151 (MQ=255)                                                                                                                              agTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATgcg                  <  1:448162/151‑1 (MQ=255)                                                                                                                               gTTGCTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATgcgg                 <  2:475545/151‑1 (MQ=255)                                                                                                                                   cTGTTGTTCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTc             >  2:176478/1‑151 (MQ=255)                                                                                                                                          tCTGGTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCCAt        >  2:577362/1‑149 (MQ=255)                                                                                                                                            tGGTTGTGGCGCGCCAGTCGCGGGGTACCAGCCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAggg    >  2:91073/1‑151 (MQ=255)                                                                                                                                             ggTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGt   <  1:634358/151‑1 (MQ=255)                                                                                                                                              gTTGTGGCGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTc  <  2:73599/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                GCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTACCAGTCAGTACGGGGAGTAAAATGCGCCAACGAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTC  >  NC_000913/2595187‑2595478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑