Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,687,347	G→A	A21V (GCC→GTC)	glnB ←	regulatory protein P‑II for glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,687,347	0	G	A	100.0%	187.7 / NA	50	A21V (GCC→GTC)	glnB	regulatory protein P‑II for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/33);  total (17/33)

CAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGGCCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCTTGAAATCGT  >  NC_000913/2687201‑2687485                                                                                                                                                   |                                                                                                                                           cAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGAcc                                                                                                                                          >  2:626714/1‑149 (MQ=255)          ccGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTcgc                                                                                                                                <  1:481577/150‑1 (MQ=255)               aCGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACa                                                                                                                           <  2:195587/150‑1 (MQ=255)               aCGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACAt                                                                                                                          <  1:169066/151‑1 (MQ=255)                cGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATc                                                                                                                         <  1:550153/151‑1 (MQ=255)                    aaTCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTcc                                                                                                                     <  1:172019/151‑1 (MQ=255)                             tttCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGaa                                                                                                             <  1:208614/150‑1 (MQ=255)                              ttCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAg                                                                                                            <  2:539370/150‑1 (MQ=255)                                  cTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGttt                                                                                                       >  1:418148/1‑151 (MQ=255)                                    ttCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGtttt                                                                                                      <  1:612526/150‑1 (MQ=255)                                     tCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTta                                                                                                     <  1:216594/150‑1 (MQ=255)                                                 ccaccaTATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAAt                                                                                        <  2:644253/151‑1 (MQ=255)                                                     caTATACTCGGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATTATCGCATCACTCtt                                                                                     <  2:633719/150‑1 (MQ=255)                                                      atatACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCtttt                                                                                   <  2:373345/151‑1 (MQ=255)                                                        ataCTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCttttt                                                                                  <  2:618487/150‑1 (MQ=255)                                                          aCTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCAt                                                                               >  2:415761/1‑151 (MQ=255)                                                             ccgcgccgcgGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCt                                                                            <  2:441500/151‑1 (MQ=255)                                                              cgcgccgcgGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCt                                                                            >  1:421771/1‑150 (MQ=255)                                                              cgcgccgcgGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTa                                                                           <  2:571823/151‑1 (MQ=255)                                                               gcgccgcgGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTAt                                                                          <  2:223781/151‑1 (MQ=255)                                                               gcgccgcgGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTAt                                                                          >  2:206425/1‑151 (MQ=255)                                                               gcgccgcgGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTAt                                                                          >  1:105413/1‑151 (MQ=255)                                                                      ggTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGa                                                                   <  1:419529/151‑1 (MQ=255)                                                                           aGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAgg                                                              >  1:118043/1‑151 (MQ=255)                                                                            gCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGt                                                             <  2:462770/151‑1 (MQ=255)                                                                                ggTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGcc                                                         <  2:476981/151‑1 (MQ=255)                                                                                ggTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGcc                                                         <  1:592479/151‑1 (MQ=255)                                                                                 gTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCt                                                        >  2:472268/1‑151 (MQ=255)                                                                                      gCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCtt                                                   <  1:168555/151‑1 (MQ=255)                                                                                         tttCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTtat                                                                                                    <  2:183339/99‑1 (MQ=255)                                                                                         tttCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTtat                                                                                                    >  1:183344/1‑99 (MQ=255)                                                                                          ttCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCtttt                                                 <  2:179377/149‑1 (MQ=255)                                                                                           tCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGAt                                              >  2:442009/1‑151 (MQ=255)                                                                                            cTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGATTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTg                                                >  2:377325/1‑148 (MQ=255)                                                                                              ggCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAATGTCGCCTGTCTTTTGAt                                              <  1:9968/148‑1 (MQ=255)                                                                                                cGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCt                                         <  1:70185/151‑1 (MQ=255)                                                                                                            ttCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAacac                              <  1:73521/150‑1 (MQ=255)                                                                                                             tCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACAt                            >  1:175486/1‑151 (MQ=255)                                                                                                                  tCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGc                       >  2:509046/1‑151 (MQ=255)                                                                                                                     gTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCaa                    <  2:366005/151‑1 (MQ=255)                                                                                                                       cACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAatt                  >  1:140729/1‑151 (MQ=255)                                                                                                                       cACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAatt                  <  2:101035/151‑1 (MQ=255)                                                                                                                       cACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAatt                  <  2:17181/151‑1 (MQ=255)                                                                                                                             cATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTcc            >  2:135819/1‑151 (MQ=255)                                                                                                                              aTGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCt           <  2:293877/151‑1 (MQ=255)                                                                                                                              aTGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCt           >  1:293884/1‑151 (MQ=255)                                                                                                                              aTGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCt           >  2:162035/1‑151 (MQ=255)                                                                                                                                gCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCtt          <  2:340107/150‑1 (MQ=255)                                                                                                                                gCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCTTg         <  1:22268/151‑1 (MQ=255)                                                                                                                                       aTACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCTTGAAATCGt  <  2:101069/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                           CAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGGCCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCTTGAAATCGT  >  NC_000913/2687201‑2687485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑