Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,688,468	C→T	A112A (GCG→GCA)	glrR ←	response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,688,468	0	C	T	100.0%	103.0 / NA	26	A112A (GCG→GCA)	glrR	response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/13);  total (13/13)

TCAAAACGCTGACGTCTGATTGCGCCACCAGCCGCGCCTGTTCCAGCAAACGCAGCATCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCG  >  NC_000913/2688320‑2688615                                                                                                                                                     |                                                                                                                                                    tCAAAACGCTGACGTCTGATTGCGCCACCAGCCGCGCCTGTTCCAGCAAACGCAGCATCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAgtgc                                                                                                                                                   >  2:78581/1‑151 (MQ=255)           gACGTCTGATTGCGCCACCAGCCGCGCCTGTTCCAGCAAACGCAGCATCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCg                                                                                                                                         >  1:460237/1‑151 (MQ=255)                 tGATTGCGCCACCAGCCGCGCCTGTTCCAGCAAACGCAGCATCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGc                                                                                                                                   >  2:496592/1‑151 (MQ=255)                                   cgcCTGTTCCAGCAAACGCAGCATCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTaaaa                                                                                                                 <  2:548697/151‑1 (MQ=255)                                        gTTCCAGCAAACGCAGCATCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGcc                                                                                                            <  2:341386/151‑1 (MQ=255)                                           ccAGCAAACGCAGCATCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCct                                                                                                          <  2:332493/150‑1 (MQ=255)                                                     cagcaTCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTgca                                                                                               <  2:610713/151‑1 (MQ=255)                                                      agcaTCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTgcag                                                                                              <  1:463988/151‑1 (MQ=255)                                                          tCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCACCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGTTATAGTGCGTCTTTGCCGACAGGATTGCTGAGGAAACTAAAAACGCACTCCTGTGTTGCAGCaa                                                                                           >  2:398944/1‑150 (MQ=255)                                                           cAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACg                                                                                         <  2:356376/151‑1 (MQ=255)                                                           cAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACg                                                                                         >  2:442855/1‑151 (MQ=255)                                                                ggCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATc                                                                                    <  1:201736/151‑1 (MQ=255)                                                                          gACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGa                                                                           >  2:513153/1‑150 (MQ=255)                                                                             aaTTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAAcc                                                                        >  2:667842/1‑150 (MQ=255)                                                                                        gcCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTa                                                              >  2:122875/1‑149 (MQ=255)                                                                                           aGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGaat                                                         >  2:508235/1‑151 (MQ=255)                                                                                            gCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGaat                                                         <  1:597045/150‑1 (MQ=255)                                                                                                        ggctggcGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCAtt                                            <  1:442866/151‑1 (MQ=255)                                                                                                                  gATTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCa                                  <  2:231071/151‑1 (MQ=255)                                                                                                                   aTTGCTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCAc                                 >  2:624015/1‑151 (MQ=255)                                                                                                                       cTCCAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTc                             >  2:1738/1‑151 (MQ=255)                                                                                                                         ccAGCGCATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTg                           <  2:499815/151‑1 (MQ=255)                                                                                                                               cATCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTc                     <  1:321903/151‑1 (MQ=255)                                                                                                                                aTCGTCAATTGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCa                    <  2:504803/151‑1 (MQ=255)                                                                                                                                        ttGCCTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAAc              >  2:591051/1‑149 (MQ=255)                                                                                                                                            cTGATATAGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGc        >  2:36204/1‑151 (MQ=255)                                                                                                                                                  taGTGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCg  >  2:640096/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TCAAAACGCTGACGTCTGATTGCGCCACCAGCCGCGCCTGTTCCAGCAAACGCAGCATCAGCGGGCTGCGGGTGACAATTGCCTCGCGCCAGCGTTCATCGGTGGCTGGCGCGGATTGCTCCAGCGCATCGTCAATTGCCTGATATAGCGCGTCTTTGTCGACAGGCTTGGTGAGGAAACTAAAAACGCCCTGCTGTGTTGCAGCAACGGCATCGGGAATAGAACCATGCGCGGTAAGAATAATTACCGGCATTCCCGGCTGCACTTTCTGGATTTCAGCAAACAGCTGCATACCG  >  NC_000913/2688320‑2688615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑