Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,747,020 G→A A259V (GCG→GTG)  ffh ← Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,747,0200GA93.5% 152.7 / NA 46A259V (GCG→GTG) ffhSignal Recognition Particle (SRP) component with 4.5S RNA (ffs)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (26/17);  minor base C (0/3);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 7.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCG  >  NC_000913/2746878‑2747164
                                                                                                                                              |                                                                                                                                                
atCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGATTGCCAGTGATGTGACGAATAGAGAGcaccgcaccg                                                                                                                                          >  1:470121/1‑151 (MQ=255)
      gATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCgg                                                                                                                                    >  1:451659/1‑151 (MQ=255)
       aTCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCggg                                                                                                                                   <  2:447891/151‑1 (MQ=255)
        tCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGc                                                                                                                                  >  2:602140/1‑151 (MQ=255)
             gACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGc                                                                                                                             >  1:342097/1‑151 (MQ=255)
                     gTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACt                                                                                                                     >  2:72198/1‑151 (MQ=255)
                       cGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACttt                                                                                                                   <  1:381439/151‑1 (MQ=255)
                        gCCCATGCCGGGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTg                                                                                                                  >  1:35199/1‑151 (MQ=255)
                          ccATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGt                                                                                                                >  2:613151/1‑151 (MQ=255)
                              gCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAac                                                                                                            >  2:538135/1‑151 (MQ=255)
                               ccGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAac                                                                                                            >  2:42858/1‑150 (MQ=255)
                                 gagaATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACt                                                                                                         >  2:306405/1‑151 (MQ=255)
                                                 aggCGGACCGGATGGTACGGCTCCCGCGCCTCAGCTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTCATGTGACGAATAGAGAGCACAGCACCGCCGCGGGCATCTTCGGCCACTTTGGTCAACACTACGCCGGTGCGCGGt                                                                                          >  2:310327/3‑150 (MQ=255)
                                                  tGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACCCCGAGGAACTTGATGGGTTTGCCATTGATGTGACGAATAGAGAGCCCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTa                                                                                         <  2:493703/150‑1 (MQ=255)
                                                      gTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGAGGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGc                                                                                     <  1:538416/150‑1 (MQ=255)
                                                        ccGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCtt                                                                                   <  2:582123/150‑1 (MQ=255)
                                                        ccGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCtt                                                                                   <  1:291585/150‑1 (MQ=255)
                                                        ccGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGAGATGTGACGAATAGAGCGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAAAACTACGCCGGTAAGCGGTAACGCTTc                                                                                  >  1:408356/1‑151 (MQ=255)
                                                         cGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCa                                                                                 <  2:212013/151‑1 (MQ=255)
                                                         cGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCa                                                                                 <  1:271869/151‑1 (MQ=255)
                                                                 aCGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGc                                                                         >  2:593945/1‑151 (MQ=255)
                                                                   ggCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGAACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCtt                                                                       <  1:406669/151‑1 (MQ=255)
                                                                       ccAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGc                                                                   <  1:604529/151‑1 (MQ=255)
                                                                         aGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCg                                                                 >  1:459906/1‑151 (MQ=255)
                                                                           cgcCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGt                                                                >  2:276178/1‑150 (MQ=255)
                                                                              cTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTg                                                            >  2:27109/1‑151 (MQ=255)
                                                                               tCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTgg                                                           >  1:360912/1‑151 (MQ=255)
                                                                                 aGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGcc                                                         <  2:212042/151‑1 (MQ=255)
                                                                                         cGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTg                                                 <  2:662320/151‑1 (MQ=255)
                                                                                          gCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATc                                                    <  2:464296/147‑1 (MQ=255)
                                                                                            cAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGAc                                               >  2:122488/1‑150 (MQ=255)
                                                                                              acacCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCgg                                            >  2:143887/1‑151 (MQ=255)
                                                                                                 ccGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTc                                          >  1:558484/1‑150 (MQ=255)
                                                                                                  cGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCAt                                        >  1:390934/1‑151 (MQ=255)
                                                                                                   gAGGAACTTGATCGGTTTGCCAGTGATGTGGCGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAAAACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATg                                       >  2:520356/1‑151 (MQ=255)
                                                                                                    aGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATgg                                      <  2:573573/151‑1 (MQ=255)
                                                                                                              tCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACcac                            >  1:438124/1‑151 (MQ=255)
                                                                                                                      ccAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAggg                    <  2:37559/151‑1 (MQ=255)
                                                                                                                       cAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGt                   <  1:10043/151‑1 (MQ=255)
                                                                                                                       cAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGt                   <  1:399227/151‑1 (MQ=255)
                                                                                                                        aGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGtt                  >  2:78010/1‑151 (MQ=255)
                                                                                                                                gACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCgg          >  1:389013/1‑151 (MQ=255)
                                                                                                                                    aaTAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGtt       >  1:349545/1‑150 (MQ=255)
                                                                                                                                     aTAGAGAGCCCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTaa     <  2:96721/151‑1 (MQ=255)
                                                                                                                                       agagagCCCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATc   <  2:132023/151‑1 (MQ=255)
                                                                                                                                         agagCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCg  <  2:212517/150‑1 (MQ=255)
                                                                                                                                         agagCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCg  >  2:211600/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                
ATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCG  >  NC_000913/2746878‑2747164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑