Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,765,124	G→A	A10A (GCC→GCT)	yfjL ←	CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,765,124	0	G	A	100.0%	147.6 / NA	38	A10A (GCC→GCT)	yfjL	CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/24);  total (14/24)

TTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCGGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACTTCACAGCCATTCTCGGTCGCAGGTATC  >  NC_000913/2764976‑2765271                                                                                                                                                     |                                                                                                                                                    ttACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTggcagc                                                                                                                                                   >  1:489867/1‑151 (MQ=255)    ccTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTAc                                                                                                                                                <  2:650263/151‑1 (MQ=255)         aCGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGc                                                                                                                                           >  1:612933/1‑151 (MQ=255)          cGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGcc                                                                                                                                          >  2:657259/1‑151 (MQ=255)                                 aCAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAg                                                                                                                    <  1:27223/150‑1 (MQ=255)                                               gTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAAtt                                                                                                     >  2:342240/1‑151 (MQ=255)                                                tCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAAtt                                                                                                     <  2:102911/150‑1 (MQ=255)                                                     gACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGa                                                                                               <  2:641462/151‑1 (MQ=255)                                                     gACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGa                                                                                               <  1:513690/151‑1 (MQ=255)                                                          cGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAAt                                                                                           <  2:354422/150‑1 (MQ=255)                                                           gCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAAtt                                                                                          >  2:143165/1‑150 (MQ=255)                                                                gcACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGgag                                                                                    <  2:26068/151‑1 (MQ=255)                                                                 cacaCTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGgaga                                                                                   <  1:13120/151‑1 (MQ=255)                                                                   cacTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATg                                                                                 <  1:397317/151‑1 (MQ=255)                                                                         ggATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGa                                                                           <  2:519657/151‑1 (MQ=255)                                                                                 ttGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTcc                                                                   >  1:442612/1‑151 (MQ=255)                                                                                 ttGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTcc                                                                   >  2:275338/1‑151 (MQ=255)                                                                                 ttGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTcc                                                                   >  2:263135/1‑151 (MQ=255)                                                                                  tGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTcc                                                                   <  2:168265/150‑1 (MQ=255)                                                                                     cACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATc                                                               >  1:100548/1‑151 (MQ=255)                                                                                       cGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCgg                                                             <  2:380855/151‑1 (MQ=255)                                                                                         aaGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGAt                                                           <  2:643327/151‑1 (MQ=255)                                                                                         aaGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGAt                                                           >  2:14883/1‑151 (MQ=255)                                                                                          aGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATc                                                          <  2:308389/151‑1 (MQ=255)                                                                                              ttCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTg                                                      >  1:227958/1‑151 (MQ=255)                                                                                               tCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTg                                                      <  2:627441/150‑1 (MQ=255)                                                                                                 gggTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGcat                                                   >  2:574856/1‑151 (MQ=255)                                                                                                 gggTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGcat                                                   <  1:295232/151‑1 (MQ=255)                                                                                                  ggTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGcatc                                                  <  1:293813/151‑1 (MQ=255)                                                                                                           gTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGt                                         >  1:364497/1‑151 (MQ=255)                                                                                                             aaGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTg                                        <  2:71338/150‑1 (MQ=255)                                                                                                                     aCGCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAAt                                >  1:551640/1‑150 (MQ=255)                                                                                                                       gCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACt                             >  2:542021/1‑151 (MQ=255)                                                                                                                       gCAGTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACt                             <  1:367995/151‑1 (MQ=255)                                                                                                                          gTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGGCATCCGTGCCAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATGGGATCACTGCATCATCACCAGTGATCTAAATACTTc                           <  2:156292/150‑1 (MQ=255)                                                                                                                          gTCCTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACTTca                          <  1:605783/151‑1 (MQ=255)                                                                                                                            ccTGAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACTTcaca                        <  2:595797/151‑1 (MQ=255)                                                                                                                               gAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACTTCACAGcc                     <  2:190491/151‑1 (MQ=255)                                                                                                                               gAAATAAGAAACCGTGCTTGGCAGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACTTCACAGc                      <  1:330789/150‑1 (MQ=255)                                                                                                                                                  ggcagcTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACTTCACAGCCATTCTCGGTCGCAGGTATc  <  2:132497/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TTACCTGAACGAAGTCGATGTATCCATCAGATACAATGTCGATATCGTCTGTGACCTCGCAGCGCACACTGCGGATAGTCTTGTCACGAAGCATTCGGGTGACATGGTAAGCGGCTACGCAGTCCTGAAATAAGAAACCGTGCTTGGCGGCTACTCCGCCTGAATCGTTCGACTCCATTTAGACATCCGTGACAATTTTGCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACTTCACAGCCATTCTCGGTCGCAGGTATC  >  NC_000913/2764976‑2765271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑