Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,801,859	A→G	Q171R (CAG→CGG)	nrdE →	ribonucleoside‑diphosphate reductase 2, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,801,859	0	A	G	100.0%	161.2 / NA	45	Q171R (CAG→CGG)	nrdE	ribonucleoside‑diphosphate reductase 2, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/24);  total (21/24)

CTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACGCGGCGGCGGCGTAGCATTTTTGCTGTCGAATCTGCGAGAAGCGGGCGCG  >  NC_000913/2801714‑2802004                                                                                                                                                  |                                                                                                                                                  cTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGcgcg                                                                                                                                              >  2:332113/1‑151 (MQ=255)   ggAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGTcgcg                                                                                                                                              <  2:633016/149‑1 (MQ=255)   ggAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGcgcg                                                                                                                                              <  2:112241/149‑1 (MQ=255)   ggAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGcgcg                                                                                                                                              <  1:589053/149‑1 (MQ=255)           ttGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTgg                                                                                                                                     <  1:14241/150‑1 (MQ=255)            tGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGtt                                                                                                                                   >  2:653330/1‑151 (MQ=255)             gCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGttt                                                                                                                                  <  2:478395/151‑1 (MQ=255)               cGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTcc                                                                                                                                >  1:179953/1‑151 (MQ=255)               cGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTcc                                                                                                                                <  1:514529/151‑1 (MQ=255)                     aGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGtttt                                                                                                                          >  2:623599/1‑151 (MQ=255)                      gTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGttttt                                                                                                                         <  2:592213/151‑1 (MQ=255)                          cGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTtgct                                                                                                                     >  1:216802/1‑151 (MQ=255)                                    gcTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGaa                                                                                                           <  1:117519/151‑1 (MQ=255)                                       gACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGa                                                                                                         <  1:309495/150‑1 (MQ=255)                                          gCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAat                                                                                                     >  1:647599/1‑151 (MQ=255)                                             ggCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAatat                                                                                                   <  2:319338/150‑1 (MQ=255)                                               cacaAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGa                                                                                                <  1:455440/151‑1 (MQ=255)                                                acaAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCt                                                                                                                               <  2:405967/119‑1 (MQ=255)                                                acaAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCt                                                                                                                               >  1:405978/1‑119 (MQ=255)                                                acaAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATgg                                                                                                 >  1:600640/1‑149 (MQ=255)                                                     gCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGAt                                                                                          <  1:22744/151‑1 (MQ=255)                                                        aTGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTgg                                                                                       <  2:384966/151‑1 (MQ=255)                                                                       tGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAAtt                                                                         <  1:48086/150‑1 (MQ=255)                                                                       tGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTc                                                                        >  2:324850/1‑151 (MQ=255)                                                                             tGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACt                                                                  >  2:449054/1‑151 (MQ=255)                                                                              gACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCAc                                                                   <  2:130847/149‑1 (MQ=255)                                                                              gACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTg                                                                 >  2:649482/1‑151 (MQ=255)                                                                              gACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTg                                                                 >  2:50826/1‑151 (MQ=255)                                                                              gACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTg                                                                 >  1:575900/1‑151 (MQ=255)                                                                                    tGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTg                                                           >  2:66274/1‑151 (MQ=255)                                                                                         tGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGa                                                       >  1:236917/1‑150 (MQ=255)                                                                                         tGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGa                                                       >  2:515571/1‑150 (MQ=255)                                                                                         tGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGa                                                       >  2:103528/1‑150 (MQ=255)                                                                                                        ttCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACgcggcggcggcg                                        <  2:11833/150‑1 (MQ=255)                                                                                                        ttCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACgcggcggcggc                                         <  1:50445/149‑1 (MQ=255)                                                                                                         tCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACGCGGCGGCGGCGt                                       >  1:543013/1‑150 (MQ=255)                                                                                                           agccagccACGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACGCGGCGGCGGCGTAGc                                    >  2:78390/1‑151 (MQ=255)                                                                                                               agccaCGCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACGCGGCGGCGGCGTAGCAttt                                >  2:259322/1‑151 (MQ=255)                                                                                                                     gCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACGCGGCGGCGGCGTAGCATTTTTGCTg                          <  2:56302/151‑1 (MQ=255)                                                                                                                     gCCAACATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACGCGGCGGCGGCGTAGCATTTTTGCTg                          >  1:367979/1‑151 (MQ=255)                                                                                                                        aaCATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACGCGGCGGCGGCGTAGCATTTTTGCTGTCg                       <  2:652350/151‑1 (MQ=255)                                                                                                                         aCATTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACGCGGCGGCGGCGTAGCATTTTTGCTGTCGa                      <  1:532500/151‑1 (MQ=255)                                                                                                                           aTTCCTCAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACGCGGCGGCGGCGTAGCATTTTTGCTGTCGAAt                    <  1:609750/151‑1 (MQ=255)                                                                                                                                 cAACTGCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACGCGGCGGCGGCGTAGCATTTTTGCTGTCGAATCTGCga              <  2:399614/151‑1 (MQ=255)                                                                                                                                      gCGGTAAGCAGCGGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACGCGGCGGCGGCGTAGCATTTTTGCTGTCGAATCTGCGAGAAg          <  1:405915/150‑1 (MQ=255)                                                                                                                                             gcagcggcGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACGCGGCGGCGGCGTAGCATTTTTGCTGTCGAATCTGCGAGAAGCGGgcgcg  <  1:329208/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CTGGAAGATTTTGCCGATCGAGTAACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCTGTCGAAACGCGGCGGCGGCGTAGCATTTTTGCTGTCGAATCTGCGAGAAGCGGGCGCG  >  NC_000913/2801714‑2802004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑