Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,805,310	T→C	S166P (TCT→CCT)	proV →	glycine betaine/proline ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,805,310	0	T	C	100.0%	140.6 / NA	37	S166P (TCT→CCT)	proV	glycine betaine/proline ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/18);  total (19/18)

CTTAATGCCGCATATGACCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGCGAAAC  >  NC_000913/2805162‑2805454                                                                                                                                                     |                                                                                                                                                 cTTAATGCCGCATATGACCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAActccct                                                                                                                                                >  2:304946/1‑151 (MQ=255)  ttAATGCCGCATATGACCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTg                                                                                                                                               >  2:39894/1‑151 (MQ=255)           cATATGACCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGc                                                                                                                                      <  1:216117/151‑1 (MQ=255)                 aCCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAAc                                                                                                                                >  2:418651/1‑151 (MQ=255)                 aCCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAAc                                                                                                                                >  1:327299/1‑151 (MQ=255)                   cGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCTGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGTGATGCGTCAAc                                                                                                                                >  2:385702/1‑149 (MQ=255)                               tACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGcc                                                                                                                  >  2:581618/1‑151 (MQ=255)                                  tGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCcgc                                                                                                               >  1:127692/1‑151 (MQ=255)                                    cGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCcgcgc                                                                                                             <  1:478416/151‑1 (MQ=255)                                     gTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCcgcgcg                                                                                                            <  1:278769/151‑1 (MQ=255)                                        cGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGAGCGtt                                                                                                          >  2:255289/1‑150 (MQ=255)                                          gTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGc                                                                                                       <  1:490451/151‑1 (MQ=255)                                                   tGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATc                                                                                               <  1:426740/150‑1 (MQ=255)                                                     gCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCgg                                                                                            >  2:48048/1‑151 (MQ=255)                                                        ggAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGata                                                                                         <  1:359789/151‑1 (MQ=255)                                                        ggAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGat                                                                                          >  1:546198/1‑150 (MQ=255)                                                               aTGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATattatt                                                                                  >  1:289497/1‑151 (MQ=255)                                                                 gCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTaa                                                                                <  2:505660/151‑1 (MQ=255)                                                                   cGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATg                                                                              <  2:19997/151‑1 (MQ=255)                                                                     aagaaCGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATgg                                                                             >  1:459646/1‑150 (MQ=255)                                                                             cGGGAAAAAGCACTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGAAGCGTCAACCCGTGGGATTAGCCTGCGCGTTAGCGATTAATCCGGAAATATTATTAATGGACGa                                                                         >  2:334732/1‑146 (MQ=255)                                                                                    aaGCCCTTGATGCACTGCGTCAGGTGGGGCTGGAAAATTATGCCCACGGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGgc                                                             <  2:399080/151‑1 (MQ=255)                                                                                    aaGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGgc                                                             >  1:494284/1‑151 (MQ=255)                                                                                     aGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGgcg                                                            <  2:639331/151‑1 (MQ=255)                                                                                            gATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTAGa                                                       <  1:465005/149‑1 (MQ=255)                                                                                                       tCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAAAGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCg                                           >  2:221460/1‑150 (MQ=255)                                                                                                        cAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCa                                         <  1:311608/151‑1 (MQ=255)                                                                                                             cGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCga                                     <  1:474948/150‑1 (MQ=255)                                                                                                             cGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTCGGATTAGCCCGCGCGTTAGCGATTATTCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCg                                      >  2:601925/1‑149 (MQ=255)                                                                                                                    gAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAgg                             <  1:401213/151‑1 (MQ=255)                                                                                                                    gAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAgg                             <  2:533300/151‑1 (MQ=255)                                                                                                                    gAAAATTATGCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAgg                             <  1:304953/151‑1 (MQ=255)                                                                                                                             gCCCACAGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTgg                    >  2:609120/1‑151 (MQ=255)                                                                                                                                cacaGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTaa                 <  1:441415/151‑1 (MQ=255)                                                                                                                                 acaGCTACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTaa                 >  2:125707/1‑150 (MQ=255)                                                                                                                                      tACCCGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTAc           >  1:80273/1‑151 (MQ=255)                                                                                                                                          cGGATGAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGc       <  1:84376/151‑1 (MQ=255)                                                                                                                                               gAACTCCCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGCGAAAc  >  1:481881/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CTTAATGCCGCATATGACCGTGCTGGACAATACTGCGTTCGGTATGGAATTGGCCGGAATTAATGCCGAAGAACGCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGCGAAAC  >  NC_000913/2805162‑2805454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑