Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,809,534	T→C	intergenic (+41/‑83)	ygaY → / → ygaZ	pseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein/putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,809,534	0	T	C	100.0%	133.5 / NA	38	intergenic (+41/‑83)	ygaY/ygaZ	pseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein/putative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/17);  total (21/17)

AACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAA  >  NC_000913/2809390‑2809675                                                                                                                                                 |                                                                                                                                              aaCATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGGGGCGAGGGTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGtt                                                                                                                                            >  1:160680/1‑148 (MQ=255)     tGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGc                                                                                                                                     >  2:456771/1‑151 (MQ=255)                  ggCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTCAGAAGCCCATCAGTCTGAt                                                                                                                        >  1:156091/1‑151 (MQ=255)                         gTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGtt                                                                                                                  >  2:561613/1‑150 (MQ=255)                             ggctggcGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGa                                                                                                              <  1:141211/150‑1 (MQ=255)                              gctggcGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAg                                                                                                               <  2:459875/148‑1 (MQ=255)                                         gaTTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTc                                                                                                 <  1:160780/151‑1 (MQ=255)                                               ccTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTta                                                                                           <  2:678084/151‑1 (MQ=255)                                                              ggtctggtGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATc                                                                             <  1:167486/150‑1 (MQ=255)                                                              ggtctggtGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCg                                                                            >  2:214238/1‑151 (MQ=255)                                                                  tggtggCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTa                                                                         >  1:293474/1‑150 (MQ=255)                                                                   ggtggCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTaa                                                                        <  1:279836/150‑1 (MQ=255)                                                                      ggCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGt                                                                    <  2:293396/151‑1 (MQ=255)                                                                        cGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTac                                                                  <  2:413242/151‑1 (MQ=255)                                                                         gAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTaca                                                                 <  2:390814/151‑1 (MQ=255)                                                                         gAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTac                                                                  >  1:208816/1‑150 (MQ=255)                                                                                 cATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATgg                                                         >  2:442348/1‑151 (MQ=255)                                                                                    cGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGaa                                                       <  2:91850/150‑1 (MQ=255)                                                                                    cGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGaa                                                       >  2:190332/1‑150 (MQ=255)                                                                                     gTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAg                                                     <  1:317981/151‑1 (MQ=255)                                                                                      tCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGc                                                    >  2:386198/1‑151 (MQ=255)                                                                                      tCAGGAAGCCGCAAAGTAAACAATCAGAAACCGTTTTCGTGGCTTTACACTTATAAAGGCGTTAAGAAGACCATTAGTCTGATAAGGTTAAGATATTCATTCAGCCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGaa                                                       <  2:522068/148‑1 (MQ=255)                                                                                        aGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGccc                                                  >  2:521302/1‑151 (MQ=255)                                                                                          gAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCt                                                 >  2:60715/1‑150 (MQ=255)                                                                                                  aaaTTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCcac                                          >  1:293238/1‑149 (MQ=255)                                                                                                   aaTTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGc                                       <  2:526178/151‑1 (MQ=255)                                                                                                          cAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTcc                                >  2:669206/1‑151 (MQ=255)                                                                                                           aaTCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCt                               >  2:381054/1‑151 (MQ=255)                                                                                                             tCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTgg                             >  2:198190/1‑151 (MQ=255)                                                                                                                gCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTc                          <  2:463782/151‑1 (MQ=255)                                                                                                                  aaCCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCgg                        >  2:33958/1‑151 (MQ=255)                                                                                                                           cGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTc                >  2:147212/1‑150 (MQ=255)                                                                                                                           cGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCa               <  1:297091/151‑1 (MQ=255)                                                                                                                                  ttACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAgg        >  2:403307/1‑151 (MQ=255)                                                                                                                                     cacTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATg     <  2:429580/151‑1 (MQ=255)                                                                                                                                     cacTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATg     <  2:269041/151‑1 (MQ=255)                                                                                                                                      acTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGc    >  1:78348/1‑151 (MQ=255)                                                                                                                                           taAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCaa  >  1:210363/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                              AACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAA  >  NC_000913/2809390‑2809675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑