Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,812,356	T→C	G310G (GGT→GGC)	emrA →	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,812,356	0	T	C	100.0%	126.6 / NA	35	G310G (GGT→GGC)	emrA	multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/12);  total (23/12)

GATTGCCAATATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGTAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTCAGGTACTGGCAAATAA  >  NC_000913/2812212‑2812499                                                                                                                                                 |                                                                                                                                                gATTGCCAATATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTgg                                                                                                                                           >  2:497134/1‑151 (MQ=255) gATTGCCAATATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTgg                                                                                                                                           <  2:216133/151‑1 (MQ=255)       cAATATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAACGCGACCGGCAACTGGATCaa                                                                                                                                      >  1:364864/1‑150 (MQ=255)        aaTATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCaaa                                                                                                                                     <  2:60458/150‑1 (MQ=255)                         gCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCt                                                                                                                   >  2:220832/1‑151 (MQ=255)                           cGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCtgt                                                                                                                 >  1:635166/1‑151 (MQ=255)                           cGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCtgt                                                                                                                 <  2:364248/151‑1 (MQ=255)                           cGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCtgt                                                                                                                 <  1:62896/151‑1 (MQ=255)                                       ccacGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACt                                                                                                     >  2:576274/1‑151 (MQ=255)                                        cacGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTg                                                                                                    <  1:647040/151‑1 (MQ=255)                                         acGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTgg                                                                                                   <  2:21422/151‑1 (MQ=255)                                          cGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGa                                                                                                  <  2:608851/151‑1 (MQ=255)                                            gATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGAcc                                                                                                >  2:644006/1‑151 (MQ=255)                                                 ttACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGaa                                                                                            <  1:350626/150‑1 (MQ=255)                                                 ttACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGaa                                                                                            >  1:573485/1‑150 (MQ=255)                                                         atgatgTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTgg                                                                                    >  2:559125/1‑150 (MQ=255)                                                           gatgTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAg                                                                                  <  1:229960/150‑1 (MQ=255)                                                            atgTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGc                                                                                 >  1:544048/1‑150 (MQ=255)                                                                    tACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGc                                                                        >  1:548100/1‑151 (MQ=255)                                                                     acacCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGc                                                                        <  2:679628/150‑1 (MQ=255)                                                                       acCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGc                                                                     >  2:362582/1‑151 (MQ=255)                                                                                gTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGtt                                                            >  1:587827/1‑151 (MQ=255)                                                                                          tGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGc                                                   >  2:3234/1‑150 (MQ=255)                                                                                             atatGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGt                                               <  2:658728/151‑1 (MQ=255)                                                                                              tatGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTg                                              >  1:5628/1‑151 (MQ=255)                                                                                                         gTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATAc                                   >  1:76186/1‑151 (MQ=255)                                                                                                            gcgcGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCa                                 >  2:81067/1‑150 (MQ=255)                                                                                                                   ctcACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGt                         >  1:579767/1‑151 (MQ=255)                                                                                                                       cTGCTTCCAGCGCAAAATGCTACCGGCAACTGGATCACAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGAc                      >  1:273713/1‑150 (MQ=255)                                                                                                                        tGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACgg                    >  1:65552/1‑151 (MQ=255)                                                                                                                           ttCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTc                  >  1:597515/1‑150 (MQ=255)                                                                                                                             ccAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTCAgg               >  1:146195/1‑151 (MQ=255)                                                                                                                                   cAAAATGCGACCGGCAACTGTATCAAAGAGGATCAGCGGGTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGGAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGGGCGTCAATACCACTAACCGTGACGGTCAGGCACt           >  2:19370/1‑149 (MQ=255)                                                                                                                                          cGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTCAGGTACTGGCAAATaa  <  1:22597/151‑1 (MQ=255)                                                                                                                                          cGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTCAGGTACTGGCAAATa   >  1:345495/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                GATTGCCAATATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGTAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTCAGGTACTGGCAAATAA  >  NC_000913/2812212‑2812499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑