Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,913,196	T→C	K152K (AAA→AAG)	relA ←	(p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,913,196	0	T	C	100.0%	167.5 / NA	46	K152K (AAA→AAG)	relA	(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/22);  total (24/22)

GTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACG  >  NC_000913/2913049‑2913343                                                                                                                                                    |                                                                                                                                                    gTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTtg                                                                                                                                                  <  1:295955/151‑1 (MQ=255)    aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTtgatg                                                                                                                                               >  1:202836/1‑151 (MQ=255)          cAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACg                                                                                                                                         >  2:79666/1‑151 (MQ=255)                       aGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAatcat                                                                                                                            >  2:310006/1‑151 (MQ=255)                       aGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAatcat                                                                                                                            <  1:547761/151‑1 (MQ=255)                        gTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAatcatc                                                                                                                           <  1:560297/151‑1 (MQ=255)                          tGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCg                                                                                                                          <  1:330535/150‑1 (MQ=255)                          tGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCg                                                                                                                          <  1:38586/150‑1 (MQ=255)                               gATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATc                                                                                                                    >  1:371453/1‑151 (MQ=255)                                   ccGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGcc                                                                                                                 >  2:459085/1‑150 (MQ=255)                                           gTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATc                                                                                                        <  1:116159/151‑1 (MQ=255)                                           gTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATc                                                                                                        <  2:184748/151‑1 (MQ=255)                                            ttAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATcc                                                                                                       >  2:635809/1‑151 (MQ=255)                                             tAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATcc                                                                                                       <  1:110169/150‑1 (MQ=255)                                             tAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATcc                                                                                                       <  2:467744/150‑1 (MQ=255)                                                  aGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGa                                                                                                  >  1:501055/1‑150 (MQ=255)                                                    cGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACg                                                                                               >  2:198055/1‑151 (MQ=255)                                                        gCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTa                                                                                            <  1:523337/150‑1 (MQ=255)                                                        gCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTAt                                                                                           <  1:600098/151‑1 (MQ=255)                                                             gATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGAcc                                                                                      >  2:122980/1‑151 (MQ=255)                                                                 ttGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGtt                                                                                  >  2:655230/1‑151 (MQ=255)                                                                 ttGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGtt                                                                                  >  1:7615/1‑151 (MQ=255)                                                                   ggTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTc                                                                                 <  2:376958/150‑1 (MQ=255)                                                                    gTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCgg                                                                               <  2:524849/151‑1 (MQ=255)                                                                      acacTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCgga                                                                              <  1:421749/150‑1 (MQ=255)                                                                                           acGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTc                                                        <  1:529388/151‑1 (MQ=255)                                                                                                      ccGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTg                                             >  2:395952/1‑151 (MQ=255)                                                                                                      ccGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTg                                             >  2:447639/1‑151 (MQ=255)                                                                                                        ggCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGc                                           <  2:341623/151‑1 (MQ=255)                                                                                                          cgcATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCgg                                         >  1:154248/1‑151 (MQ=255)                                                                                                            cATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGAt                                       >  1:126937/1‑151 (MQ=255)                                                                                                              tCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcg                                     >  2:167501/1‑151 (MQ=255)                                                                                                                ttttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcgcc                                   >  2:677839/1‑151 (MQ=255)                                                                                                                ttttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcgcc                                   >  1:589058/1‑151 (MQ=255)                                                                                                                 tttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcgccg                                  >  1:457604/1‑151 (MQ=255)                                                                                                                 tttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcg                                     <  2:114231/148‑1 (MQ=255)                                                                                                                 tttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATc                                      <  2:165660/147‑1 (MQ=255)                                                                                                                 tttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATc                                      <  2:233182/147‑1 (MQ=255)                                                                                                                           gcAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCGGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATcacg                        <  1:169284/151‑1 (MQ=255)                                                                                                                               aTGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATcacgcacg                    >  1:39834/1‑151 (MQ=255)                                                                                                                                         cGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATa           >  1:94370/1‑150 (MQ=255)                                                                                                                                          gCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATa           >  1:120321/1‑149 (MQ=255)                                                                                                                                          gCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATa           >  2:124076/1‑149 (MQ=255)                                                                                                                                            tCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCAAGCCGTGAATAAGGt       >  1:354285/1‑151 (MQ=255)                                                                                                                                                ccAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAAc   <  2:169867/151‑1 (MQ=255)                                                                                                                                                 cAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATACCACCCGGCGAACGTTATCGCCCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAAcg  <  2:328124/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACG  >  NC_000913/2913049‑2913343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑