Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,968,723	A→G	R85R (CGT→CGC)	rppH ←	RNA pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,968,723	0	A	G	100.0%	174.0 / NA	47	R85R (CGT→CGC)	rppH	RNA pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/25);  total (22/25)

TCTGACCGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGATTCCG  >  NC_000913/2968576‑2968864                                                                                                                                                    |                                                                                                                                              tCTGACCGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAgcgc                                                                                                                                            >  1:580705/1‑151 (MQ=255)    gACCGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCAcc                                                                                                                                         <  1:533445/151‑1 (MQ=255)       cGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCaa                                                                                                                                       >  2:47563/1‑150 (MQ=255)              cAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCg                                                                                                                               <  2:292380/151‑1 (MQ=255)                gTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGt                                                                                                                             <  2:62036/151‑1 (MQ=255)                  aaCTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTaa                                                                                                                           <  1:394740/151‑1 (MQ=255)                   aCTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAAt                                                                                                                          <  2:19125/151‑1 (MQ=255)                   aCTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAAt                                                                                                                          <  1:58980/151‑1 (MQ=255)                       aCCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGt                                                                                                                      >  2:441550/1‑151 (MQ=255)                          cATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAgc                                                                                                                   >  1:255477/1‑151 (MQ=255)                           aTCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAgcg                                                                                                                  >  1:10851/1‑151 (MQ=255)                             cGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAgcgc                                                                                                                 >  2:563317/1‑150 (MQ=255)                                 aGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAAcc                                                                                                             >  2:101189/1‑150 (MQ=255)                                 aGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCa                                                                                                            <  2:190467/151‑1 (MQ=255)                                   ccGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGt                                                                                                          >  1:588665/1‑151 (MQ=255)                                     gTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTa                                                                                                        <  1:360928/151‑1 (MQ=255)                                       cAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAATCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTAc                                                                                                       <  1:519406/150‑1 (MQ=255)                                        aaaCTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTAcgc                                                                                                     >  2:134040/1‑151 (MQ=255)                                         aaCTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTAcgcg                                                                                                    <  1:608616/151‑1 (MQ=255)                                             ctGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGtt                                                                                                  <  2:126205/149‑1 (MQ=255)                                                  gtACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCaa                                                                                           <  2:587555/151‑1 (MQ=255)                                                     ctgctgGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAgg                                                                                         <  2:44863/150‑1 (MQ=255)                                                            tttGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAAc                                                                                 >  2:321130/1‑151 (MQ=255)                                                                      gATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCgg                                                                       >  1:175218/1‑151 (MQ=255)                                                                        tttCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCt                                                                     >  1:299089/1‑151 (MQ=255)                                                                          tCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCtt                                                                    <  2:134951/150‑1 (MQ=255)                                                                                   ccgcTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTActt                                                          <  2:627379/151‑1 (MQ=255)                                                                                       tCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACttcttc                                                      >  1:162331/1‑151 (MQ=255)                                                                                        cACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACttcttc                                                      <  1:322528/150‑1 (MQ=255)                                                                                          ccAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCaaa                                                   <  1:405415/151‑1 (MQ=255)                                                                                           cAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAAc                                                  <  2:532916/151‑1 (MQ=255)                                                                                               tGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAAtt                                              >  1:497124/1‑151 (MQ=255)                                                                                                  aaGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCAc                                           >  2:280917/1‑151 (MQ=255)                                                                                                      gaAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTa                                       <  1:358239/151‑1 (MQ=255)                                                                                                         aCCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACAt                                    >  2:539777/1‑151 (MQ=255)                                                                                                                    ttttgGCCGCTACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGGCTTTGCGGCTTAATCCTAATTCTTCAAACAATTCACGGTACATCGCCTGctc                           >  2:514275/1‑149 (MQ=255)                                                                                                                    ttttgGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTg                         >  2:309080/1‑151 (MQ=255)                                                                                                                    ttttgGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTg                         >  1:280006/1‑151 (MQ=255)                                                                                                                      ttgGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGc                        <  2:190817/150‑1 (MQ=255)                                                                                                                        ggCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGa                     <  2:76636/151‑1 (MQ=255)                                                                                                                            gATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATtct                 >  1:673058/1‑151 (MQ=255)                                                                                                                                 aaaCCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCggg            >  2:422583/1‑151 (MQ=255)                                                                                                                                  aaCCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCgggg           <  1:56204/151‑1 (MQ=255)                                                                                                                                       gCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGAt      <  2:334746/151‑1 (MQ=255)                                                                                                                                       gCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGAt      >  2:559358/1‑151 (MQ=255)                                                                                                                                         ttCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGATTc    >  2:14616/1‑151 (MQ=255)                                                                                                                                          tCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGATTc    <  1:412098/150‑1 (MQ=255)                                                                                                                                           cGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGATTCCg  <  1:557708/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              TCTGACCGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGATTCCG  >  NC_000913/2968576‑2968864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑