Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,068,614	C→T	S65S (TCG→TCA)	argO ←	arginine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,068,614	0	C	T	97.7%	150.2 / NA	43	S65S (TCG→TCA)	argO	arginine transporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (28/14);  total (29/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAATTTTCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGCGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAG  >  NC_000913/3068473‑3068760                                                                                                                                              |                                                                                                                                                   gATAATTTTCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATc                                                                                                                                           >  1:455571/1‑151 (MQ=255) gATAATTTTCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATc                                                                                                                                           <  2:447245/151‑1 (MQ=255)  aTAATTTTCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATc                                                                                                                                           >  1:453068/1‑150 (MQ=255)        ttCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAAcg                                                                                                                                    >  1:301720/1‑151 (MQ=255)        ttCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAAc                                                                                                                                     <  1:504983/150‑1 (MQ=255)         tCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGTCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAAcgc                                                                                                                                   >  1:9288/1‑151 (MQ=255)          ccATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAAcgcg                                                                                                                                  <  2:467105/151‑1 (MQ=255)                      cTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCaaa                                                                                                                       >  1:336526/1‑150 (MQ=255)                        tCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCaaaa                                                                                                                      <  1:530101/149‑1 (MQ=255)                         cATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATc                                                                                                                   >  1:278329/1‑151 (MQ=255)                          aTGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATc                                                                                                                   <  1:627834/150‑1 (MQ=255)                            gACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATccc                                                                                                                 <  2:595770/150‑1 (MQ=255)                             aCTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCgg                                                                                                               >  1:448224/1‑151 (MQ=255)                                                 aaTATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCg                                                                                           >  1:310486/1‑151 (MQ=255)                                                 aaTATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCg                                                                                           >  1:226482/1‑151 (MQ=255)                                                    atTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCt                                                                                         <  2:675295/150‑1 (MQ=255)                                                     ttACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCCAATCAGGACCAAATCGCTg                                                                                        >  2:629090/1‑150 (MQ=255)                                                     ttACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTg                                                                                        >  2:251493/1‑150 (MQ=255)                                                      tACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGAt                                                                                      >  2:428485/1‑151 (MQ=255)                                                      tACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGAt                                                                                      >  2:449682/1‑151 (MQ=255)                                                       aCTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGAt                                                                                      >  2:160750/1‑150 (MQ=255)                                                                tGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGt                                                                            >  2:287151/1‑151 (MQ=255)                                                                     ttttAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGc                                                                       >  2:84992/1‑151 (MQ=255)                                                                       ttAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCa                                                                      >  1:613168/1‑150 (MQ=255)                                                                       ttAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCa                                                                      >  2:644237/1‑150 (MQ=255)                                                                       ttAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCa                                                                      >  2:542705/1‑150 (MQ=255)                                                                           aaGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCAt                                                                 <  1:400268/151‑1 (MQ=255)                                                                              cgcCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAAt                                                              <  1:549997/151‑1 (MQ=255)                                                                                ccAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAAtgt                                                            >  1:627912/1‑151 (MQ=255)                                                                                ccAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAAtgt                                                            <  2:336516/151‑1 (MQ=255)                                                                                  aaaaCCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTgg                                                          >  1:378226/1‑151 (MQ=255)                                                                                     aCCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGCGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTa                                                        >  2:108280/1‑150 (MQ=255)                                                                                        aTACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTg                                                     >  2:550246/1‑150 (MQ=255)                                                                                          aCCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTgac                                                   <  2:162114/150‑1 (MQ=255)                                                                                                 cAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATg                                           <  1:401270/151‑1 (MQ=255)                                                                                                     aagGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCt                                       >  2:214450/1‑151 (MQ=255)                                                                                                          tACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATtc                                  >  2:499607/1‑151 (MQ=255)                                                                                                                  cccAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACaaa                          <  2:215649/151‑1 (MQ=255)                                                                                                                     aGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGc                       >  1:377101/1‑151 (MQ=255)                                                                                                                                  cAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCg          >  2:450256/1‑151 (MQ=255)                                                                                                                                   aGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCg          >  1:562888/1‑150 (MQ=255)                                                                                                                                   aGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCACGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCg          >  2:102397/1‑150 (MQ=255)                                                                                                                                          aCGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAg  <  2:99284/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                   GATAATTTTCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGCGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAG  >  NC_000913/3068473‑3068760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑