Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,088,189	T→C	intergenic (+329/‑95)	metK → / → galP	S‑adenosylmethionine synthetase/D‑galactose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,088,189	0	T	C	100.0%	113.8 / NA	31	intergenic (+329/‑95)	metK/galP	S‑adenosylmethionine synthetase/D‑galactose transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/9);  total (22/9)

ATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTTCG  >  NC_000913/3088047‑3088335                                                                                                                                               |                                                                                                                                                   atTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGAt                                                                                                                                             <  1:652860/150‑1 (MQ=255)   tttCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTa                                                                                                                                           <  2:588631/150‑1 (MQ=255)    ttCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTGTTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTac                                                                                                                                          >  1:619905/1‑150 (MQ=255)      cATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACt                                                                                                                                       >  2:13456/1‑151 (MQ=255)                          acaGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATc                                                                                                                   >  2:514391/1‑151 (MQ=255)                                gttACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCttttt                                                                                                              >  2:480139/1‑150 (MQ=255)                                                gTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTAt                                                                                             >  2:156175/1‑151 (MQ=255)                                                  aaaGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCt                                                                                           >  1:423195/1‑151 (MQ=255)                                                   aaGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCtt                                                                                          <  1:132536/151‑1 (MQ=255)                                                      tCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAt                                                                                       >  2:534307/1‑151 (MQ=255)                                                         tAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAt                                                                                       <  1:374899/148‑1 (MQ=255)                                                          aaTTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTcac                                                                                   >  2:61199/1‑151 (MQ=255)                                                           aTTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTcac                                                                                   >  1:40631/1‑150 (MQ=255)                                                             tGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACaa                                                                                 <  2:497096/150‑1 (MQ=255)                                                             tGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAAt                                                                                >  2:146708/1‑151 (MQ=255)                                                               cAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTTAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATa                                                                               >  1:357228/1‑150 (MQ=255)                                                               cAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATaa                                                                              <  2:591947/151‑1 (MQ=255)                                                                            ttAATCTATGATGATATAACTCAATTATTTTCATGCTCTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAACTCACAATAAAAAATAACCata                                                                  <  2:142457/150‑1 (MQ=255)                                                                              aaTCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTg                                                               >  1:467780/1‑151 (MQ=255)                                                                                   atgatgATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAgg                                                           <  2:661332/150‑1 (MQ=255)                                                                                           ataACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGc                                                  >  2:138957/1‑151 (MQ=255)                                                                                                       ttttCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTaaa                                       >  2:181506/1‑150 (MQ=255)                                                                                                        tttCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTaaaa                                      >  1:262934/1‑150 (MQ=255)                                                                                                          tCATGCACTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAAc                                   >  1:6630/1‑151 (MQ=255)                                                                                                                aCTTAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGc                             >  1:620420/1‑151 (MQ=255)                                                                                                                   tAAATCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCgg                           <  1:272186/150‑1 (MQ=255)                                                                                                                      aTCATAACTAAGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCa                        >  1:571866/1‑150 (MQ=255)                                                                                                                               aaGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCa               >  2:451658/1‑150 (MQ=255)                                                                                                                                aGATAAATGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAAt             >  2:159370/1‑151 (MQ=255)                                                                                                                                     aaTGTTAGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGt        >  1:437035/1‑151 (MQ=255)                                                                                                                                           aGTGCAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGCCAAACAAGGCAATGACGTTTTTCg  >  1:351899/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                   ATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTTCG  >  NC_000913/3088047‑3088335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑