Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	474,975	A→G	Y63H (TAC→CAC)	tesB ←	acyl‑CoA thioesterase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	474,975	0	A	G	100.0%	205.9 / NA	55	Y63H (TAC→CAC)	tesB	acyl‑CoA thioesterase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/27);  total (28/27)

CTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTCAATTTTTT  >  NC_000913/474828‑475118                                                                                                                                                    |                                                                                                                                                cTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCt                                                                                                                                              >  2:429342/1‑151 (MQ=255)    gTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAACTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCt                                                                                                                                              >  2:560764/1‑148 (MQ=255)       ccTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGaaa                                                                                                                                        <  1:251403/151‑1 (MQ=255)       ccTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGaaa                                                                                                                                        <  2:23033/151‑1 (MQ=255)          ggAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAAc                                                                                                                                       <  2:303180/149‑1 (MQ=255)          ggAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAAc                                                                                                                                       <  1:432884/149‑1 (MQ=255)          ggAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAAc                                                                                                                                       <  2:424348/149‑1 (MQ=255)          ggAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACg                                                                                                                                      >  2:86063/1‑150 (MQ=255)            aaaGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAAt                                                                                                                                   >  2:102876/1‑151 (MQ=255)               gagGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGt                                                                                                                                 >  1:470328/1‑150 (MQ=255)                  gCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTAcc                                                                                                                              >  1:301749/1‑150 (MQ=255)                      tCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGcc                                                                                                                          >  2:24388/1‑150 (MQ=255)                              aaaaTCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTc                                                                                                                   >  2:189478/1‑149 (MQ=255)                                   cGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACg                                                                                                            <  1:539156/151‑1 (MQ=255)                                             ttttGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTg                                                                                                  >  1:651133/1‑151 (MQ=255)                                               ttGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTgc                                                                                                 <  2:358894/150‑1 (MQ=255)                                                  aaTAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTgcagc                                                                                              >  2:384057/1‑150 (MQ=255)                                                    tAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCAt                                                                                            >  1:606037/1‑150 (MQ=255)                                                     agcagcAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATa                                                                                           <  2:335876/150‑1 (MQ=255)                                                       cagcaACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACaa                                                                                        <  2:613062/151‑1 (MQ=255)                                                       cagcaACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACaa                                                                                        <  2:368042/151‑1 (MQ=255)                                                         gcaACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGAACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAgg                                                                                      >  2:438794/1‑151 (MQ=255)                                                            aCCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCt                                                                                   >  1:84536/1‑151 (MQ=255)                                                              ccGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGa                                                                                 <  2:452846/151‑1 (MQ=255)                                                                ggcgggcgCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGTCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGAcc                                                                               <  2:155328/151‑1 (MQ=255)                                                                          gAAGCTGTTACCGGCACGCAGCGTTTCGACATCATAAATAAGCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTg                                                                      <  2:482563/150‑1 (MQ=255)                                                                                 ttACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGgccgcc                                                                <  1:541976/149‑1 (MQ=255)                                                                                       tCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCt                                                        >  2:473600/1‑151 (MQ=255)                                                                                        cACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACAccgg                                                       <  1:574453/151‑3 (MQ=255)                                                                                         aCGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTgg                                                      <  2:394336/151‑1 (MQ=255)                                                                                          cGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGc                                                     <  1:416472/151‑1 (MQ=255)                                                                                           gCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCg                                                    <  2:50805/151‑1 (MQ=255)                                                                                                 tttCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAAcc                                              >  1:146969/1‑151 (MQ=255)                                                                                                      aCATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAAt                                         >  1:79946/1‑151 (MQ=255)                                                                                                       catcatAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATc                                        >  1:667888/1‑151 (MQ=255)                                                                                                        atcatAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCt                                       <  1:623441/151‑1 (MQ=255)                                                                                                          catAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTc                                     >  1:570115/1‑151 (MQ=255)                                                                                                             aaataaTCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCAct                                  <  2:306064/151‑1 (MQ=255)                                                                                                                    cGGCTTCTTACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCcg                           >  1:268171/1‑151 (MQ=255)                                                                                                                        ttcttACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGGCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCcgcg                         >  1:322745/1‑149 (MQ=255)                                                                                                                           ttACTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGaa                       >  2:315644/1‑148 (MQ=255)                                                                                                                             aCTATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGt                  >  2:69508/1‑151 (MQ=255)                                                                                                                               tATCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGtcc                >  1:466429/1‑151 (MQ=255)                                                                                                                                aTCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGtcct               <  1:474596/151‑1 (MQ=255)                                                                                                                                 tCGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCATGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGtcctt              >  2:24122/1‑151 (MQ=255)                                                                                                                                  cGCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGtccttc             >  1:675165/1‑151 (MQ=255)                                                                                                                                   gCCAGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGtccttcc            >  1:656784/1‑151 (MQ=255)                                                                                                                                      aGGGCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTc          >  1:481053/1‑150 (MQ=255)                                                                                                                                       gggCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTCa         <  2:141523/150‑1 (MQ=255)                                                                                                                                       gggCGAAGAAAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTCa         <  2:621494/150‑1 (MQ=255)                                                                                                                                            aagaaAGTGGCTGTGAAACGAATGTCCCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTCAAttttt   <  2:506178/151‑1 (MQ=255)                                                                                                                                            aagaaAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTCAAttttt   <  2:26266/151‑1 (MQ=255)                                                                                                                                            aagaaAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTCAAttttt   <  1:139527/151‑1 (MQ=255)                                                                                                                                            aagaaAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTCAAttt     <  1:659206/149‑1 (MQ=255)                                                                                                                                             agaaAGTGGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTCAAtttttt  >  2:14805/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                CTGGTGCCTGGAAAGAGGCAGTCATATAAAAAATCGGTTTGCCGTTTTGAATAGCAGCAACCCGGCGGGCGCTGAAGCTGTTACCGTCACGCAGCGTTTCGACATCATAAATAATCGGCTTCTTACTATCGCCAGGGCGAAGAAAGTAGCTGTGAAACGAATGTACCAGCCGCTCTTCAGGGACGGTCTCTTTTGCAGCATACAAGGCCTGACCCACGACCTGGCCGCCAAACACCTGGCGTAAACCTAAATCTTCACTCTGGCCGCGAAAGAGTCCTTCCTCAATTTTTT  >  NC_000913/474828‑475118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑