Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,235,399	G→A	L159L (CTC→CTT)	rlmG ←	23S rRNA m(2)G1835 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,235,399	0	G	A	100.0%	182.3 / NA	47	L159L (CTC→CTT)	rlmG	23S rRNA m(2)G1835 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/27);  total (20/27)

AGACATTCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGAT  >  NC_000913/3235254‑3235542                                                                                                                                                  |                                                                                                                                                agaCATTCGCATGGTTGTGGATAGTCCAGGCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGCTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCAc                                                                                                                                            >  2:542285/1‑151 (MQ=255)     aTTCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACtttt                                                                                                                                        <  1:92458/151‑1 (MQ=255)       tCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACttttt                                                                                                                                       >  1:605744/1‑150 (MQ=255)        cGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCTCTTTTTc                                                                                                                                      <  2:358779/150‑1 (MQ=255)         gCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGa                                                                                                                                    <  2:529079/151‑1 (MQ=255)                tgtgGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGtt                                                                                                                              <  1:311385/150‑1 (MQ=255)                tgtgGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTc                                                                                                                             >  1:306501/1‑151 (MQ=255)                   ggATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCa                                                                                                                           <  2:112209/150‑1 (MQ=255)                    gATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGc                                                                                                                         >  1:227810/1‑151 (MQ=255)                     aTAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCg                                                                                                                        >  2:430328/1‑151 (MQ=255)                        gTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGGGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTgg                                                                                                                     >  2:167712/1‑151 (MQ=255)                           cagtcagtACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAg                                                                                                                  <  1:108260/151‑1 (MQ=255)                                   aCCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAAt                                                                                                           <  1:228477/150‑1 (MQ=255)                                      ttccagcttccagcTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTc                                                                                                        >  2:110257/1‑150 (MQ=255)                                        ccagcttccagctAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACg                                                                                                     <  2:299230/151‑1 (MQ=255)                                              tgcagctGACGGTCTGCGGGGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCATGGTGGTGGTTGGGCCAAGCACTGTTTCGCGCAGCTCGAGCGTGGAAGTGTGAATGTCACggggc                                                                                                 <  2:60086/147‑3 (MQ=255)                                                cagctAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTgg                                                                                             >  1:210302/1‑151 (MQ=255)                                                     aaCGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCg                                                                                        <  2:429399/151‑1 (MQ=255)                                                       cGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGc                                                                                      <  1:655971/151‑1 (MQ=255)                                                        ggTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCa                                                                                     <  1:633585/151‑1 (MQ=255)                                                                  gcATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACgtg                                                                           >  2:71342/1‑151 (MQ=255)                                                                  gcATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACgtg                                                                           <  1:333804/151‑1 (MQ=255)                                                                    aTCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTa                                                                         <  1:504525/151‑1 (MQ=255)                                                                      cGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATc                                                                       >  1:172203/1‑151 (MQ=255)                                                                              tGCGGTTGATTGAAAGTGGAATTAATCAGGCGAGCTTATTTCCATGGCAGCGTGGTGGTGGTGGGGCCAAGCACTTTTTCGAACAGTTCCGGCGTGGAAGTGTAAATGTCACGGGCCTTGGCACCGGCAATAACACGTGTAACCGACGTg                                                                <  2:564501/150‑1 (MQ=255)                                                                                      aTTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTg                                                       >  2:217639/1‑151 (MQ=255)                                                                                        tGAAAGAGCAATTAATTAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAGGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTgcg                                                     >  2:679510/1‑151 (MQ=255)                                                                                         gAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTgcgc                                                    >  2:606256/1‑151 (MQ=255)                                                                                               gCAATTAATCAGGCGCGCCTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGTGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCCCTTTgcgcagcgc                                               <  2:229369/150‑1 (MQ=255)                                                                                                 aaTTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGcgcacg                                            >  1:337726/1‑151 (MQ=255)                                                                                                  aTTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGcgcacgc                                           >  2:351167/1‑151 (MQ=255)                                                                                                      aTCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGtt                                       <  2:233071/151‑1 (MQ=255)                                                                                                      aTCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGtt                                       >  1:131472/1‑151 (MQ=255)                                                                                                      aTCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGtg                                       <  2:76237/151‑2 (MQ=255)                                                                                                       tCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTg                                      >  1:623766/1‑151 (MQ=255)                                                                                                          ggCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTg                                   <  2:340350/151‑1 (MQ=255)                                                                                                           gcgcgcTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGt                                  <  2:369689/151‑1 (MQ=255)                                                                                                           gcgcgcTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGt                                  >  2:151986/1‑151 (MQ=255)                                                                                                            cgcgcTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGtt                                 <  2:249127/151‑1 (MQ=255)                                                                                                               gcTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCa                              <  2:556549/151‑1 (MQ=255)                                                                                                                 ttttttCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGc                            >  1:163578/1‑151 (MQ=255)                                                                                                                     ttCCATGCCAGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAAt                         <  2:390053/150‑1 (MQ=255)                                                                                                                              aGCGTGGTGGTGGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGttt               >  2:397820/1‑151 (MQ=255)                                                                                                                                 gtggtggtgGTTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCg            <  1:563272/151‑1 (MQ=255)                                                                                                                                       gtggtTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACtt      <  1:414314/151‑1 (MQ=255)                                                                                                                                         ggtTGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTg    <  2:216779/151‑1 (MQ=255)                                                                                                                                            tGGGCCAAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGAt  <  2:672533/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                AGACATTCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAATGCCAGTGTTTTCGGCACTTTGAT  >  NC_000913/3235254‑3235542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑