Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,252,977	T→C	Y23H (TAC→CAC)	yhaI →	DUF805 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,252,977	0	T	C	100.0%	62.8 / NA	17	Y23H (TAC→CAC)	yhaI	DUF805 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/6);  total (11/6)

AAGATACGAATAGCATTTACACAATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGTACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGC  >  NC_000913/3252842‑3253110                                                                                                                                        |                                                                                                                                      aagaTACGAATAGCATTTACACAATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTActc                                                                                                                        >  2:383694/1‑151 (MQ=255)      aCGAATAGCATTTACACAATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGAtt                                                                                                                   >  2:628290/1‑151 (MQ=255)             gCATTTACACAATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGcc                                                                                                             >  2:76597/1‑150 (MQ=255)              cATTTACACAATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCAt                                                                                                           <  2:63318/151‑1 (MQ=255)                     acaATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGgc                                                                                                    >  2:649106/1‑151 (MQ=255)                        aTACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCt                                                                                                 >  1:491324/1‑151 (MQ=255)                             cGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCtattat                                                                                            <  1:62705/151‑1 (MQ=255)                                     aCTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATgac                                                                                     >  1:237235/1‑148 (MQ=255)                                             cACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTg                                                                            <  2:568478/151‑1 (MQ=255)                                                                         cAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGt                                                >  1:629203/1‑151 (MQ=255)                                                                            tGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCt                                              >  2:620580/1‑150 (MQ=255)                                                                                aTTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGc                                          <  1:296378/150‑1 (MQ=255)                                                                                    gTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTAt                                     >  2:670073/1‑151 (MQ=255)                                                                                          aTTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTg                               <  1:658998/151‑1 (MQ=255)                                                                                           ttAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTgg                              >  1:260568/1‑151 (MQ=255)                                                                                                           gTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGt              <  1:620599/151‑1 (MQ=255)                                                                                                                       gcgcACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGc  >  1:297084/1‑151 (MQ=255)                                                                                                                                        |                                                                                                                                      AAGATACGAATAGCATTTACACAATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGTACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGC  >  NC_000913/3252842‑3253110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑