Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,362,012	A→G	intergenic (+72/‑100)	gltF → / → yhcA	periplasmic protein/putative periplasmic chaperone protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,362,012	0	A	G	100.0%	179.3 / NA	48	intergenic (+72/‑100)	gltF/yhcA	periplasmic protein/putative periplasmic chaperone protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/22);  total (26/22)

GACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGACATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTATTTATAACAACATCAATGA  >  NC_000913/3361866‑3362157                                                                                                                                                   |                                                                                                                                                  gACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCAt                                                                                                                                                <  2:70700/150‑1 (MQ=255) gACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATg                                                                                                                                               <  1:449605/151‑1 (MQ=255) gACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATg                                                                                                                                               <  1:614021/151‑1 (MQ=255)    aaTACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGgtc                                                                                                                                            >  2:204986/1‑151 (MQ=255)    aaTACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGgtc                                                                                                                                            <  1:581793/151‑1 (MQ=255)      tACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGgtctg                                                                                                                                          >  2:104570/1‑151 (MQ=255)        cTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGgtctgtc                                                                                                                                        >  2:44347/1‑151 (MQ=255)         tATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGgtctgtct                                                                                                                                       >  1:21491/1‑151 (MQ=255)           ttCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCtt                                                                                                                                      >  2:629421/1‑150 (MQ=255)            tCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTa                                                                                                                                     <  1:204991/150‑1 (MQ=255)                    cTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAAtctc                                                                                                                            >  2:3120/1‑151 (MQ=255)                        tgaAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCg                                                                                                                        >  2:320482/1‑151 (MQ=255)                          aaaCGATCAAACTGGATGGTAACGTCACTATCCGCGTTCAGTATTTATAAGAAATTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAACTAAAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATc                                                                                                                         <  2:420144/148‑1 (MQ=255)                           aaCGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCa                                                                                                                      >  1:588508/1‑150 (MQ=255)                                        gATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACa                                                                                                        <  1:223232/151‑1 (MQ=255)                                              aaCGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACatatat                                                                                                   <  1:573978/150‑1 (MQ=255)                                               aCGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTa                                                                                                 <  1:478460/151‑1 (MQ=255)                                                  tCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAAc                                                                                               >  1:603881/1‑150 (MQ=255)                                                   cACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACaa                                                                                             <  2:219913/151‑1 (MQ=255)                                                   cACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACaa                                                                                             <  1:397256/151‑1 (MQ=255)                                                   cACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACa                                                                                              >  2:301846/1‑150 (MQ=255)                                                          aGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGttt                                                                                      <  2:426237/151‑1 (MQ=255)                                                            cGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGATCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCt                                                                                    <  2:126112/151‑1 (MQ=255)                                                                 aGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGtt                                                                               <  1:197556/151‑1 (MQ=255)                                                                    aTTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGttatt                                                                            >  1:614864/1‑151 (MQ=255)                                                                       tataAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGttattta                                                                          <  2:163339/150‑1 (MQ=255)                                                                          aaGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCat                                                                      >  2:212820/1‑151 (MQ=255)                                                                             aaCTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCattat                                                                   >  2:234821/1‑151 (MQ=255)                                                                                 tGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTAc                                                                >  2:317621/1‑150 (MQ=255)                                                                                 tGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTAc                                                                >  1:531686/1‑150 (MQ=255)                                                                                  gTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAg                                                              <  1:657501/151‑1 (MQ=255)                                                                                     aTACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAg                                                           <  2:616172/151‑1 (MQ=255)                                                                                     aTACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAg                                                           <  1:606279/151‑1 (MQ=255)                                                                                      tACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGt                                                          >  1:636051/1‑151 (MQ=255)                                                                                         aGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGtttt                                                       >  1:261969/1‑151 (MQ=255)                                                                                                atttttaCGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTtttaattta                                                 >  2:283463/1‑150 (MQ=255)                                                                                                        gCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTc                                          >  2:72849/1‑149 (MQ=255)                                                                                                        gCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAg                                        <  1:92678/151‑1 (MQ=255)                                                                                                            tAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGacac                                    >  2:109111/1‑151 (MQ=255)                                                                                                               ccAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACacatt                                 >  2:81966/1‑151 (MQ=255)                                                                                                                     aGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTc                           <  1:88239/151‑1 (MQ=255)                                                                                                                     aGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTc                           >  1:461851/1‑151 (MQ=255)                                                                                                                       cTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCAc                         >  2:365152/1‑151 (MQ=255)                                                                                                                              tCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTAttt                  >  1:379810/1‑151 (MQ=255)                                                                                                                                      aTTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTACTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTATTTATaacaac          <  1:611788/151‑1 (MQ=255)                                                                                                                                        tATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTATTTATAACAACAt        >  2:440788/1‑151 (MQ=255)                                                                                                                                             ccTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTATTTATAACAACATCAATg   >  2:419868/1‑151 (MQ=255)                                                                                                                                                aTGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTATTTATAACAACATCAATGa  <  2:196794/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGACATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTATTTATAACAACATCAATGA  >  NC_000913/3361866‑3362157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑