Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,368,566	G→A	G247E (GGG→GAG)	yhcG →	DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,368,566	0	G	A	100.0%	173.1 / NA	45	G247E (GGG→GAG)	yhcG	DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/20);  total (25/20)

CCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGGGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATG  >  NC_000913/3368424‑3368709                                                                                                                                               |                                                                                                                                                ccTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGgatga                                                                                                                                           <  2:306844/149‑1 (MQ=255) ccTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGAtt                                                                                                                                         >  2:303209/1‑151 (MQ=255) ccTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGAtt                                                                                                                                         >  2:497592/1‑151 (MQ=255)    gCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTg                                                                                                                                      <  1:110942/151‑1 (MQ=255)       gagaCGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCt                                                                                                                                   >  2:568867/1‑151 (MQ=255)              atatTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTgg                                                                                                                            <  1:509983/151‑1 (MQ=255)                      ccGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGc                                                                                                                    <  1:493528/151‑1 (MQ=255)                                 gATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGc                                                                                                         <  2:591677/151‑1 (MQ=255)                                     cgcgATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATtg                                                                                                     >  1:327095/1‑151 (MQ=255)                                      gcgATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATtga                                                                                                    <  1:220308/151‑1 (MQ=255)                                        gATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGTATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATtgatg                                                                                                  <  1:412994/151‑1 (MQ=255)                                        gATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATtgatg                                                                                                  >  2:465934/1‑151 (MQ=255)                                          tCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGAc                                                                                                >  1:152077/1‑151 (MQ=255)                                           cccTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGAc                                                                                                >  2:427026/1‑150 (MQ=255)                                            ccTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACa                                                                                               >  2:195042/1‑150 (MQ=255)                                               ttGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACt                                                                                            <  2:277193/150‑1 (MQ=255)                                                tgtgCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTg                                                                                           <  2:33474/150‑1 (MQ=255)                                                     tCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCg                                                                                     >  1:165155/1‑151 (MQ=255)                                                      cGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCg                                                                                     >  2:311748/1‑150 (MQ=255)                                                        aaTTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCgtgt                                                                                  >  1:602233/1‑151 (MQ=255)                                                               ggAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGAt                                                                              <  1:481754/148‑1 (MQ=255)                                                               ggAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATctg                                                                           >  1:154026/1‑151 (MQ=255)                                                                 aaTTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATctgct                                                                         >  1:105142/1‑151 (MQ=255)                                                                       aaGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGttttt                                                                   >  1:270780/1‑151 (MQ=255)                                                                             aaTATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCAccg                                                             <  2:294476/151‑1 (MQ=255)                                                                                 ttCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGt                                                         >  2:512714/1‑151 (MQ=255)                                                                                   cAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGttt                                                       >  2:460253/1‑151 (MQ=255)                                                                                    aGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTa                                                      <  1:499361/151‑1 (MQ=255)                                                                                         ccGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGctg                                                 >  1:275247/1‑151 (MQ=255)                                                                                          cGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGctgc                                                >  1:588855/1‑151 (MQ=255)                                                                                               ttGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGctgcctgc                                            <  2:93739/150‑1 (MQ=255)                                                                                                 gaggagGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTa                                          <  1:662067/150‑1 (MQ=255)                                                                                                           tGATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCt                               >  1:662817/1‑151 (MQ=255)                                                                                                            gATCAACCACCTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATGGATGACAACTGGTTTCTCGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCt                               <  2:130518/150‑1 (MQ=255)                                                                                                                   caccTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTgg                       <  2:66602/151‑1 (MQ=255)                                                                                                                    accTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTggg                      >  1:395978/1‑151 (MQ=255)                                                                                                                     ccTGATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTggg                      <  2:638039/150‑1 (MQ=255)                                                                                                                        gATGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCaa                   >  1:383437/1‑150 (MQ=255)                                                                                                                         aTGGATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAAt                 >  1:678452/1‑151 (MQ=255)                                                                                                                           ggATTTCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGTCAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTc               >  2:161924/1‑151 (MQ=255)                                                                                                                               ttCATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCt           >  1:136041/1‑151 (MQ=255)                                                                                                                                 cATGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCtat         <  1:497419/151‑1 (MQ=255)                                                                                                                                  aTGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCtata        <  2:153027/151‑1 (MQ=255)                                                                                                                                  aTGCTGGAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCtata        <  2:327088/151‑1 (MQ=255)                                                                                                                                        gAACTTGAGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATg  >  1:551907/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                CCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAGAATCCGATTTTGAGGAGGCGCTGATCAACCACCTGATGGATTTCATGCTGGAACTTGGGGATGATTTTGCCTTTGTTGGTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATG  >  NC_000913/3368424‑3368709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑