Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	483,535	C→T	G290E (GGG→GAG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	483,535	0	C	T	100.0%	156.8 / NA	41	G290E (GGG→GAG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/23);  total (18/23)

TGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTA  >  NC_000913/483391‑483678                                                                                                                                                 |                                                                                                                                                tGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGAc                                                                                                                                           >  1:299472/1‑151 (MQ=255)     tAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCgg                                                                                                                                        >  1:119637/1‑150 (MQ=255)             ttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGtt                                                                                                                               >  1:212003/1‑151 (MQ=255)              tCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGtt                                                                                                                               <  2:91274/150‑1 (MQ=255)              tCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGtt                                                                                                                               <  2:472703/150‑1 (MQ=255)               cACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTg                                                                                                                              <  2:174145/150‑1 (MQ=255)                   aaCGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAGGCCGGTTGGCCGt                                                                                                                         <  1:2916/151‑1 (MQ=255)                     cGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTa                                                                                                                       <  2:571549/151‑1 (MQ=255)                      ggCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTa                                                                                                                       >  2:23628/1‑150 (MQ=255)                       gCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTaa                                                                                                                      >  1:437122/1‑150 (MQ=255)                        cGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAAc                                                                                                                    <  2:260143/151‑1 (MQ=255)                              gtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCg                                                                                                              <  1:168239/151‑1 (MQ=255)                                cGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCgat                                                                                                            <  1:108007/151‑1 (MQ=255)                                cGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCga                                                                                                             <  1:343546/150‑1 (MQ=255)                                  tATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCgatg                                                                                                           >  2:163653/1‑150 (MQ=255)                                      ggTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTc                                                                                                      <  1:149634/151‑1 (MQ=255)                                         aaaCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGt                                                                                                    <  1:396445/150‑1 (MQ=255)                                         aaaCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTa                                                                                                   <  1:456060/151‑1 (MQ=255)                                             aaTTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTtc                                                                                               <  1:185474/151‑1 (MQ=255)                                             aaTTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTtc                                                                                               >  1:5403/1‑151 (MQ=255)                                                      aCCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCaccacca                                                                                      <  2:645863/151‑2 (MQ=255)                                                       cccGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCAccc                                                                                      >  1:123251/1‑150 (MQ=255)                                                         cGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGc                                                                                   >  1:573070/1‑151 (MQ=255)                                                             gggAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCaa                                                                               <  1:436326/151‑1 (MQ=255)                                                             gggAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCa                                                                                >  2:236574/1‑150 (MQ=255)                                                                aagaaCGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCt                                                                            <  1:10794/151‑1 (MQ=255)                                                                              cTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgc                                                              >  1:114397/1‑151 (MQ=255)                                                                                  gCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgcagca                                                          <  1:195427/151‑1 (MQ=255)                                                                                             aCGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGa                                                >  2:662002/1‑150 (MQ=255)                                                                                              cGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAAc                                              >  1:245517/1‑151 (MQ=255)                                                                                                 aTTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCa                                            <  1:398123/150‑1 (MQ=255)                                                                                                      cGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTg                                       >  2:498098/1‑150 (MQ=255)                                                                                                                   aGCGCGTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTcagca                         <  1:476520/151‑1 (MQ=255)                                                                                                                       cgTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGAt                     <  2:142979/151‑1 (MQ=255)                                                                                                                        gTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGAtt                    >  1:69523/1‑151 (MQ=255)                                                                                                                        gTTTGCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGAtt                    >  2:304424/1‑151 (MQ=255)                                                                                                                            gCACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTg                 <  1:130578/150‑1 (MQ=255)                                                                                                                             cACCGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGcc               >  2:151566/1‑151 (MQ=255)                                                                                                                               ccGGTCGCCAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCg              >  1:54766/1‑150 (MQ=255)                                                                                                                                      ccAGCTTGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTc      <  1:15696/151‑1 (MQ=255)                                                                                                                                            tGATCTCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTa  <  1:98950/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                TGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTA  >  NC_000913/483391‑483678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑