Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,391,786	A→G	A81A (GCT→GCC)	tldD ←	putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,391,786	0	A	G	98.3%	202.5 / ‑3.8	58	A81A (GCT→GCC)	tldD	putative peptidase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (31/26);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CATGCTTTGCAGCGGATCTACCGAGGTATACAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCAGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGAAAATAGAGATCGCCATAATCAAGGCG  >  NC_000913/3391642‑3391935                                                                                                                                                 |                                                                                                                                                      cATGCTTTGCAGCGGATCTACCGAGGTATACAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTa                                                                                                                                                  <  2:612707/150‑1 (MQ=255)        tGCAGCGGATCTACCGAGGTATACAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAAt                                                                                                                                           <  1:325249/150‑1 (MQ=255)           aGCGGATCTACCGAGGTATACAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCgg                                                                                                                                       >  1:321478/1‑151 (MQ=255)             cGGATCTACCGAGGTATACAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGtt                                                                                                                                     >  1:651421/1‑151 (MQ=255)                   tACCGAGGTATACAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTc                                                                                                                                >  1:347337/1‑150 (MQ=255)                    aCCGAGGTATACAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGtttttt                                                                                                                                 <  2:28403/148‑1 (MQ=255)                    aCCGAGGTATACAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGtttt                                                                                                                                   <  2:621418/146‑1 (MQ=255)                     ccGAGGTATACAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCAcc                                                                                                                             >  2:560043/1‑151 (MQ=255)                      cGAGGTATACAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCg                                                                                                                            <  2:294185/151‑1 (MQ=255)                       gAGGTATACAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGc                                                                                                                           >  2:526440/1‑151 (MQ=255)                       gAGGTATACAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGc                                                                                                                           <  1:588112/151‑1 (MQ=255)                            ataCAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGAtt                                                                                                                      >  2:532439/1‑151 (MQ=255)                            ataCAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGAtt                                                                                                                      <  2:421243/151‑1 (MQ=255)                            ataCAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGAt                                                                                                                       <  1:524125/150‑1 (MQ=255)                                aaCGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTgcac                                                                                                                  >  1:467563/1‑151 (MQ=255)                                aaCGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTgcac                                                                                                                  <  2:639927/151‑1 (MQ=255)                                  cGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTgcacgc                                                                                                                >  1:413611/1‑151 (MQ=255)                                  cGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTgcacgc                                                                                                                >  2:473419/1‑151 (MQ=255)                                       tATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACAcc                                                                                                           >  2:132261/1‑151 (MQ=255)                                                ccGCGCCCCGCGTCTGTACTTTACCCACACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCTGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCTCCGCTGATTGCGCGCACACCAACGCCCt                                                                                                   <  2:458019/150‑1 (MQ=255)                                                ccGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTg                                                                                                  <  2:96166/151‑1 (MQ=255)                                                 cgcgCCCAGCGTCTGTACTTTACCATCACCACTACCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGa                                                                                                 <  2:44326/151‑1 (MQ=255)                                                  gcgcCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGAt                                                                                                >  1:378227/1‑151 (MQ=255)                                                       cAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGAtg                                                                                           >  1:582532/1‑151 (MQ=255)                                                       cAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGAtg                                                                                           >  2:36180/1‑151 (MQ=255)                                                        aGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGAtgt                                                                                          <  1:94871/151‑1 (MQ=255)                                                         gCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGAtgtt                                                                                         <  2:379651/151‑1 (MQ=255)                                                          cGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGAtgttg                                                                                        >  1:37896/1‑151 (MQ=255)                                                          cGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGAtgttg                                                                                        >  1:668322/1‑151 (MQ=255)                                                               gTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTa                                                                                      >  1:39867/1‑148 (MQ=255)                                                                 aCTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGaa                                                                                  <  2:17757/150‑1 (MQ=255)                                                                       ccATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCtt                                                                           <  1:14564/151‑1 (MQ=255)                                                                             ccacTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTtaata                                                                      <  1:351946/150‑1 (MQ=255)                                                                                  aTCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCgg                                                                >  2:94399/1‑151 (MQ=255)                                                                                  aTCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCgg                                                                >  1:355555/1‑151 (MQ=255)                                                                                   tCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGt                                                               >  2:503330/1‑151 (MQ=255)                                                                                      cGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGtctt                                                            <  2:530260/151‑1 (MQ=255)                                                                                      cGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGtctt                                                            <  1:567546/151‑1 (MQ=255)                                                                                         aCGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGtcttct                                                          >  2:307304/1‑150 (MQ=255)                                                                                           gATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTaa                                                        >  2:225303/1‑150 (MQ=255)                                                                                                    cgccgcTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGAt                                              <  1:289972/151‑1 (MQ=255)                                                                                                       cgcTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCg                                           <  1:402769/151‑1 (MQ=255)                                                                                                        gcTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGt                                          >  1:29719/1‑151 (MQ=255)                                                                                                            gcgcACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATa                                      <  1:361242/151‑1 (MQ=255)                                                                                                                 ctctGTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCg                                 >  1:59779/1‑151 (MQ=255)                                                                                                                     gTTCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTg                             <  1:413911/151‑1 (MQ=255)                                                                                                                       tCCAGCGCCAGCAGGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGa                            >  1:406544/1‑150 (MQ=255)                                                                                                                               cagcagGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGAAAATag                      >  1:15307/1‑148 (MQ=255)                                                                                                                                   agGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGAAAATAGAGATCGc               >  2:237831/1‑151 (MQ=255)                                                                                                                                   agGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGAAAATAGAGATCGc               <  1:556596/151‑1 (MQ=255)                                                                                                                                   agGCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGAAAATAGAGATCGc               >  1:38640/1‑151 (MQ=255)                                                                                                                                     gCTGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGAAAATAGAGATCGcc              >  2:291806/1‑150 (MQ=255)                                                                                                                                       tGATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGAAAATAGAGATCGCCAt            >  1:542125/1‑150 (MQ=255)                                                                                                                                        gATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGAAAATAGAGATCGCCATaa          <  1:628192/151‑1 (MQ=255)                                                                                                                                        gATTTGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGAAAATAGAGATCGCCATaa          <  1:388139/151‑1 (MQ=255)                                                                                                                                          tttGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATC‑TTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGAAAATAGAGATCGCCATAATc        >  1:503905/1‑150 (MQ=255)                                                                                                                                           ttGGTCGGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGAAAATAGAGATCGCCATAATc        >  2:576090/1‑150 (MQ=255)                                                                                                                                                cAGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGAAAATAGAGATCGCCATAATCAAGGCg  >  1:569328/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                      CATGCTTTGCAGCGGATCTACCGAGGTATACAACGGGCTATGCTCTACCGCGCCCAGCGTCTGTACTTTACCATCACCACTATCACGGACGATGGTGCGCGCCGCTTGCGCACTCTGTTCCAGCGCCAGCAGGCTGATTTGGTCAGCGTAAGCAAATCCGGTTTTTTCACCGCTGATTGCACGCACACCAACGCCCTGATCGATGTTGTAAGAACCATCTTTAATAATGCGGTCTTCTAAAACCCAGGATTCGTGATAGCTCGACTGAAAATAGAGATCGCCATAATCAAGGCG  >  NC_000913/3391642‑3391935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑