Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,462,301	A→G	T110A (ACG→GCG)	gspI →	general secretory pathway component, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,462,301	0	A	G	100.0%	146.9 / NA	42	T110A (ACG→GCG)	gspI	general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/23);  total (19/23)

GAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGC  >  NC_000913/3462157‑3462444                                                                                                                                                 |                                                                                                                                                gAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGtt                                                                                                                                           <  1:403590/151‑1 (MQ=255)      aaaCACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCa                                                                                                                                       >  1:674444/1‑150 (MQ=255)        acacGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAg                                                                                                                                    <  1:680016/151‑1 (MQ=255)        acacGTCCAGTTCTAGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAg                                                                                                                                    <  2:82619/151‑1 (MQ=255)               cAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGat                                                                                                                             <  1:213454/151‑1 (MQ=255)                aGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGata                                                                                                                            <  2:327802/151‑1 (MQ=255)                 gTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGataa                                                                                                                           <  1:83950/151‑1 (MQ=255)                       gCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATc                                                                                                                     >  1:62163/1‑151 (MQ=255)                                  ataaATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGcac                                                                                                          >  1:432087/1‑151 (MQ=255)                                    aaaTGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGcaca                                                                                                         >  2:506385/1‑150 (MQ=255)                                      aTGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGcaca                                                                                                         >  2:131318/1‑148 (MQ=255)                                          cGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAgatgat                                                                                                  <  1:329993/151‑1 (MQ=255)                                            aaGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTa                                                                                                <  2:339220/151‑1 (MQ=255)                                            aaGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTa                                                                                                <  2:148890/151‑1 (MQ=255)                                                   gAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCca                                                                                         <  1:108733/151‑1 (MQ=255)                                                             aGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTc                                                                               <  2:331602/151‑1 (MQ=255)                                                               cGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTcac                                                                             <  2:523811/151‑1 (MQ=255)                                                               cGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTcac                                                                             >  1:20774/1‑151 (MQ=255)                                                                gATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTcaca                                                                            <  2:569537/151‑1 (MQ=255)                                                                gATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTcac                                                                             >  1:77975/1‑150 (MQ=255)                                                                 atatCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACAt                                                                           >  1:371717/1‑151 (MQ=255)                                                                 atatCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACAt                                                                           >  2:283614/1‑151 (MQ=255)                                                                        cTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTgg                                                                     >  1:355123/1‑150 (MQ=255)                                                                          cAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGa                                                                    <  1:453088/149‑1 (MQ=255)                                                                              cAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCAt                                                              <  1:162843/151‑1 (MQ=255)                                                                               aaaGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATg                                                             >  1:411707/1‑151 (MQ=255)                                                                                 aGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATggc                                                           >  1:39802/1‑151 (MQ=255)                                                                                                ggAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGAtttt                                            >  1:431479/1‑151 (MQ=255)                                                                                                         cATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCt                                   >  2:577825/1‑151 (MQ=255)                                                                                                          aTAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCtt                                  >  1:423835/1‑151 (MQ=255)                                                                                                          aTAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCtt                                  >  1:612179/1‑151 (MQ=255)                                                                                                             aCGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGc                               <  2:116024/151‑1 (MQ=255)                                                                                                               ggTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGt                             <  2:399799/151‑1 (MQ=255)                                                                                                               ggTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGt                             >  2:677147/1‑151 (MQ=255)                                                                                                                  gACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCt                          <  1:460577/151‑1 (MQ=255)                                                                                                                   aCATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTg                         <  2:119671/151‑1 (MQ=255)                                                                                                                         cccAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGtt                    <  2:604460/150‑1 (MQ=255)                                                                                                                                  cAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAttttt          <  1:614294/151‑1 (MQ=255)                                                                                                                                    gACAGCCTCGCTTGCGCGTTATCAAAGCATCGATGAGAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCGGGGTTTCTCATGGCTGGAGGTCATGGCGGCGTTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTATc        <  2:658424/151‑1 (MQ=255)                                                                                                                                          cTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGc  >  1:628896/1‑151 (MQ=255)                                                                                                                                          cTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGc  >  2:569011/1‑151 (MQ=255)                                                                                                                                            cGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGc  <  2:125242/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                GAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGC  >  NC_000913/3462157‑3462444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑