Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,497,615	T→C	F205L (TTC→CTC)	nirC →	nitrite transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,497,615	0	T	C	100.0%	113.5 / NA	32	F205L (TTC→CTC)	nirC	nitrite transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

CAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGTTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGCCGAAAGC  >  NC_000913/3497467‑3497757                                                                                                                                                     |                                                                                                                                               cAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTc                                                                                                                                              >  2:491888/1‑151 (MQ=255)           ttGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCt                                                                                                                                    >  2:42552/1‑151 (MQ=255)               gtAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGtt                                                                                                                                <  1:649390/151‑1 (MQ=255)                  aCTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCgg                                                                                                                             >  2:84842/1‑151 (MQ=255)                              gCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCg                                                                                                                  <  1:200074/150‑1 (MQ=255)                                     aaTCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTac                                                                                                          >  2:572396/1‑151 (MQ=255)                                      aTCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTac                                                                                                          >  2:569184/1‑150 (MQ=255)                                          ggATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACg                                                                                                       <  2:397262/149‑1 (MQ=255)                                              ggCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGc                                                                                                  <  2:506102/150‑1 (MQ=255)                                                 gcTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGt                                                                                              <  2:551763/151‑1 (MQ=255)                                                    gcgcACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTAtt                                                                                           >  1:519840/1‑151 (MQ=255)                                                               gggCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACct                                                                                <  1:402839/151‑1 (MQ=255)                                                                   ggcgAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACctgct                                                                             >  1:592163/1‑150 (MQ=255)                                                                           ttATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGa                                                                     <  1:121117/150‑1 (MQ=255)                                                                                   aTCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGt                                                             <  1:309851/150‑1 (MQ=255)                                                                                       ggtggtGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTaa                                                           <  1:53886/148‑1 (MQ=255)                                                                                       ggtggtGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTa                                                            <  1:616670/147‑1 (MQ=255)                                                                                                 gctgGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGt                                              <  2:158136/151‑1 (MQ=255)                                                                                                      cATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGt                                         <  1:6358/151‑1 (MQ=255)                                                                                                      cATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGt                                         <  1:333968/151‑1 (MQ=255)                                                                                                          tATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGGGACGCTGGGGAATACTTTATCAGGTGCCGtttt                                      >  1:227499/1‑150 (MQ=255)                                                                                                            tCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGGGCCGTATTCa                                    >  1:324758/1‑150 (MQ=255)                                                                                                             cgcgTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTAtt                                      >  1:118199/1‑147 (MQ=255)                                                                                                                      cTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGGCATAACCTGCTGTGGGGGGCGCTGGGGAATACTTTATCAGGGGCCGTATTCATGGGGTTggg                          >  2:12149/1‑150 (MQ=255)                                                                                                                        aCGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGt                         <  1:84845/149‑1 (MQ=255)                                                                                                                         cGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGGGGCGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGGTTGGGGTAt                      >  1:563291/1‑151 (MQ=255)                                                                                                                            gCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTGTGGGTGACGCTGGGGAATACTTTATCAGGTGCCGTATTCATGGGGTTGGGTTATTgg                   >  1:521363/1‑151 (MQ=255)                                                                                                                                   aTCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGGCGTATTCATGGGATTGGGGTATTGGGATGCt             >  1:404202/1‑150 (MQ=255)                                                                                                                                         aaCATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGcc        <  2:379121/149‑1 (MQ=255)                                                                                                                                         aaCATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGcc        <  1:155053/149‑1 (MQ=255)                                                                                                                                         aaCATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGGGCCGTATTCATTGGGTTGGGTTTTTGGTATGCTACGCCg       >  1:487515/1‑150 (MQ=255)                                                                                                                                             tGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGCCTTCGTCCTAACCCGCTGTGGGCGACGCCCGGTAATACTTTTTCCGGTGCCCGATTCATGGGGTTGGGGTATTGGTATGCTACGCCGCAAGc  >  2:616377/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                               CAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGTTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGCCGAAAGC  >  NC_000913/3497467‑3497757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑