Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,505,884	A→G	L46P (CTG→CCG)	yhfS ←	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,505,884	0	A	G	100.0%	83.7 / NA	27	L46P (CTG→CCG)	yhfS	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/17);  total (10/17)

CCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGC  >  NC_000913/3505738‑3506026                                                                                                                                                   |                                                                                                                                                 ccAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑c                                                                                                                                              <  1:449013/150‑1 (MQ=255)  cAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCt                                                                                                                                            <  2:576421/151‑1 (MQ=255)         cGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTa                                                                                                                                     <  1:129888/151‑1 (MQ=255)             gCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAg                                                                                                                                   <  1:141384/149‑1 (MQ=255)              ccccGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCt                                                                                                                                <  2:451249/151‑1 (MQ=255)                    tttGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑Tc                                                                                                                          >  2:138223/1‑150 (MQ=255)                           aaaGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGcc                                                                                                                    >  1:14075/1‑149 (MQ=255)                               ccgccAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCTTTAAGCCTAAA‑TCACCGCCAGTAAg                                                                                                              <  1:159427/151‑1 (MQ=255)                                cgccAGCCCTGCGCGAATCGCGCCAATCCACGCGCCCTGCACCAGCGCCTCAGCCTGTGCGTGAAGTGCATCAGCCAGCACCTGTTCCACACGCTGGGGAACGCGCGTTTGATTCGGT‑CCTGTCGTTAAGCCTAAA‑TCACCGCCGGTa                                                                                                                >  2:502878/1‑148 (MQ=255)                                      cccGGCGCGAATCGCGCCATCCCCCGCCCCCTGCCCCACCCCCGCAGCCTGTGCGTAAAATCCATCACCCAGCCCTTTTCCCACACGCTGGGTACCGCGCGGTTGATTCGTT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACt                                                                                                         <  2:425344/149‑1 (MQ=255)                                                                cgcCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCCCCTGTCCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCt                                                                             <  1:193932/151‑1 (MQ=255)                                                                  ccgtGCCCCGGCGCCGCATCCTGGGTCGGAAATGCATCGGCCAGCACCGCTCCCACCCGCGGGGAAAGGCGCGG‑TGGTTGGGTCCCTGGGGTTAGGCCTAAA‑TCGCCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTAt                                                                           <  2:482382/148‑1 (MQ=255)                                                                               ccgcGGCCGGTGCGTGAAATGCATCAGCCAGCACCTGTCCCACCCGCTGGGTACCGCGCGGTTGTTTCGGT‑CCGGGCGTAAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAgc                                                                   <  2:107033/146‑1 (MQ=255)                                                                                cgcgGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCAAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAAc                                                             <  2:365909/151‑1 (MQ=255)                                                                                        gtgCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtg                                                     >  1:537760/1‑151 (MQ=255)                                                                                            gtaaaaGGCATCAGCCAGCCCCTGTCCCACCCGCGGGGTACCGCGCGGTTGATTCGGT‑CCTGGCTTAAACCCAAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtgctg                                                  <  2:536741/147‑1 (MQ=255)                                                                                            gTGAAATGCATCAGCCAGCCCGTGTCCCCCACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtgctgc                                                 <  1:335953/151‑1 (MQ=255)                                                                                                  tGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTc                                            >  2:229759/1‑150 (MQ=255)                                                                                                      tCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCaata                                        >  1:326302/1‑150 (MQ=255)                                                                                                                     tCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGc                         >  2:432813/1‑150 (MQ=255)                                                                                                                            gCTGGGTAACGCGCGGTTGTTTCGGT‑CCTGGCGTTAAGCCTAAA‑CCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGa                   <  1:140888/149‑1 (MQ=255)                                                                                                                              tGGGTACCGCGCGTTTGTTTCGGT‑CTTGGGGTTAAGCTTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAAc                <  1:188161/150‑1 (MQ=255)                                                                                                                              tGGGAAACGCGCGTTTGATCCGGT‑CCTGGCTTTAA‑CCTAAACCCGCCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCagag                     <  1:170971/145‑1 (MQ=255)                                                                                                                               gggTAACGCGCGGTTGATTCGGT‑CCGGGCGTTAAGCCAAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAAc                <  1:536369/149‑1 (MQ=255)                                                                                                                                    aCGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTc          >  2:143592/1‑150 (MQ=255)                                                                                                                                       cgcgGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCAt        >  2:520966/1‑149 (MQ=255)                                                                                                                                           gTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGc  >  1:104689/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGC  >  NC_000913/3505738‑3506026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑