Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,546,644	T→C	V29A (GTC→GCC)	gntT →	gluconate transporter, high‑affinity GNT I system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,546,644	0	T	C	100.0%	184.6 / NA	53	V29A (GTC→GCC)	gntT	gluconate transporter, high‑affinity GNT I system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/26);  total (27/26)

AATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGA  >  NC_000913/3546498‑3546788                                                                                                                                                   |                                                                                                                                                 aaTTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTc                                                                                                                                              <  1:55061/151‑1 (MQ=255)  aTTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCg                                                                                                                                             <  1:176709/151‑1 (MQ=255)     agagTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTg                                                                                                                                           <  2:396378/150‑1 (MQ=255)        gTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGgcgc                                                                                                                                       >  2:562681/1‑151 (MQ=255)         tttGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCt                                                                                                                                      >  1:213448/1‑151 (MQ=255)         tttGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCt                                                                                                                                      >  2:295838/1‑151 (MQ=255)           tGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCtt                                                                                                                                     <  1:460936/150‑1 (MQ=255)           tGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTg                                                                                                                                    >  2:536398/1‑151 (MQ=255)              cgtcAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTg                                                                                                                                 >  2:325077/1‑151 (MQ=255)                tcAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTg                                                                                                                                 <  1:231732/149‑1 (MQ=255)                 cAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTg                                                                                                                              >  1:622411/1‑151 (MQ=255)                   gAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTgg                                                                                                                             >  1:209011/1‑150 (MQ=255)                   gAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGa                                                                                                                            <  2:367605/151‑1 (MQ=255)                     aaaTTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGAtt                                                                                                                          >  1:412791/1‑151 (MQ=255)                      aaTTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGAtt                                                                                                                          >  1:528014/1‑150 (MQ=255)                          gACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATg                                                                                                                      <  1:32546/150‑1 (MQ=255)                            cGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCaa                                                                                                                   <  2:161816/151‑1 (MQ=255)                               tACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAgg                                                                                                                 <  1:79126/150‑1 (MQ=255)                                     tAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGcc                                                                                                           >  2:670089/1‑150 (MQ=255)                                      aaCAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGc                                                                                                         >  2:450947/1‑151 (MQ=255)                                         aaaTGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTgg                                                                                                      >  2:458739/1‑151 (MQ=255)                                             gAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATa                                                                                                   <  2:127645/150‑1 (MQ=255)                                               aaGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATaa                                                                                                  >  1:212362/1‑149 (MQ=255)                                               aaGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATaa                                                                                                  >  2:301907/1‑149 (MQ=255)                                                  gCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGtt                                                                                              >  1:358857/1‑150 (MQ=255)                                                   ccAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTAt                                                                                            >  2:608136/1‑151 (MQ=255)                                                        tAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCt                                                                                       >  1:674578/1‑151 (MQ=255)                                                         aaaTCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTc                                                                                      >  2:655579/1‑151 (MQ=255)                                                             cATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCAt                                                                                   <  2:387125/150‑1 (MQ=255)                                                              aTGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCa                                                                                 <  1:655600/151‑1 (MQ=255)                                                               tGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCa                                                                                 >  2:601973/1‑150 (MQ=255)                                                                gCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCaaa                                                                               <  1:627916/151‑1 (MQ=255)                                                                     tAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCg                                                                           <  2:5434/150‑1 (MQ=255)                                                                               tgCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTcggcgg                                                                 <  1:126303/150‑1 (MQ=255)                                                                               tgCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCggg                                                                >  2:366772/1‑151 (MQ=255)                                                                                gCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCggg                                                                <  1:475897/150‑1 (MQ=255)                                                                                   aTCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGAc                                                              <  1:587274/149‑1 (MQ=255)                                                                                   aTCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGc                                                            <  1:252651/151‑1 (MQ=255)                                                                                         gtACTCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGAc                                                              >  2:138993/1‑143 (MQ=255)                                                                                                   tgttgCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGccc                                             >  2:5281/1‑150 (MQ=255)                                                                                                              atgatCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGtt                                 <  2:213443/151‑1 (MQ=255)                                                                                                               tgatCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGttt                                >  2:190693/1‑151 (MQ=255)                                                                                                                gatCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGtttt                               <  1:677708/151‑1 (MQ=255)                                                                                                                gatCCGCTTCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGttt                                <  2:388581/150‑1 (MQ=255)                                                                                                                        tCAAAATGAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                         <  2:115132/149‑1 (MQ=255)                                                                                                                               gAACGGCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                >  2:34987/1‑151 (MQ=255)                                                                                                                                   ggCTTCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa            <  2:358847/151‑1 (MQ=255)                                                                                                                                      ttCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc         >  2:411558/1‑151 (MQ=255)                                                                                                                                      ttCATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc         >  2:592031/1‑151 (MQ=255)                                                                                                                                        cATCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg       <  2:444286/151‑1 (MQ=255)                                                                                                                                         aTCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg      <  2:32624/151‑1 (MQ=255)                                                                                                                                          tCGCTCTCGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAATGCTgg      >  2:192792/1‑150 (MQ=255)                                                                                                                                               ctcGCCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGa  <  2:530135/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 AATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGA  >  NC_000913/3546498‑3546788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑