Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,563,193	A→G	D394G (GAC→GGC)	glpD →	sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,563,193	0	A	G	100.0%	186.5 / NA	55	D394G (GAC→GGC)	glpD	sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

CCGCTGCTGTCGGTATTCGGCGGTAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCG  >  NC_000913/3563048‑3563339                                                                                                                                                  |                                                                                                                                                   ccGCTGCTGTCGGTATTCGGCGGTAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCcgcg                                                                                                                                               <  1:670990/151‑1 (MQ=255)      gctgTCGGTATTCGGCGGTAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGcgacg                                                                                                                                            <  1:53061/149‑1 (MQ=255)      gctgTCGGTATTCGGCGGTAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGcgacg                                                                                                                                            <  1:434446/149‑1 (MQ=255)            ggTATTCGGCGGTAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGcc                                                                                                                                    <  2:147669/151‑1 (MQ=255)                 tCGGCGGTAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTc                                                                                                                                <  2:121802/150‑1 (MQ=255)                     cggTAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTg                                                                                                                           <  2:521594/151‑1 (MQ=255)                        tAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTgcgc                                                                                                                        >  1:83189/1‑151 (MQ=255)                           gCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGcgccgc                                                                                                                     <  2:257200/151‑1 (MQ=255)                                    cTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCg                                                                                                            >  1:384864/1‑151 (MQ=255)                                      aCCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGtt                                                                                                          <  2:506249/151‑1 (MQ=255)                                       ccGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGtt                                                                                                          <  2:51375/150‑1 (MQ=255)                                         gAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCct                                                                                                       >  1:449123/1‑151 (MQ=255)                                             aCTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCctgact                                                                                                   >  2:224385/1‑151 (MQ=255)                                                  cGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATc                                                                                              >  2:275939/1‑151 (MQ=255)                                                   ggAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATc                                                                                              <  2:60033/150‑1 (MQ=255)                                                      aCATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTg                                                                                          >  1:95761/1‑151 (MQ=255)                                                       cATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTgg                                                                                         >  2:257993/1‑151 (MQ=255)                                                       cATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTgg                                                                                         >  2:207623/1‑151 (MQ=255)                                                        aTGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGgc                                                                                        <  1:96333/151‑1 (MQ=255)                                                            gcTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGt                                                                                    >  2:299669/1‑151 (MQ=255)                                                               ggAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCAt                                                                                 >  1:217694/1‑151 (MQ=255)                                                               gaaaaaaCTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCa                                                                                  <  2:189056/148‑1 (MQ=255)                                                                 aaaaaCTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCAtt                                                                                >  2:589724/1‑150 (MQ=255)                                                                     aCTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCt                                                                           >  1:230221/1‑151 (MQ=255)                                                                      cTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTc                                                                          >  2:566386/1‑151 (MQ=255)                                                                       tAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCg                                                                         >  1:374988/1‑151 (MQ=255)                                                                        aaCGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTc                                                                          <  2:268156/149‑1 (MQ=255)                                                                        aaCGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCg                                                                         >  1:104498/1‑150 (MQ=255)                                                                        aaCGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCg                                                                         <  2:139407/150‑1 (MQ=255)                                                                         aCGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGc                                                                        >  1:657845/1‑150 (MQ=255)                                                                         aCGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGc                                                                        >  1:360387/1‑150 (MQ=255)                                                                            ccGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACtt                                                                    <  1:260865/151‑1 (MQ=255)                                                                                attatCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACgg                                                                >  1:258735/1‑151 (MQ=255)                                                                                        ggTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACa                                                        <  2:304035/151‑1 (MQ=255)                                                                                          tATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGc                                                      >  1:84430/1‑151 (MQ=255)                                                                                            ttGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCg                                                     >  1:672145/1‑150 (MQ=255)                                                                                              ggCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAgc                                                  <  1:110442/151‑1 (MQ=255)                                                                                                cccGGCATGGACGAGAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGACGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAgct                                                 >  2:209229/1‑150 (MQ=255)                                                                                                cccGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAgct                                                 <  2:42377/150‑1 (MQ=255)                                                                                                       tGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTc                                          <  1:474947/150‑1 (MQ=255)                                                                                                            gAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAAt                                    <  1:453636/151‑1 (MQ=255)                                                                                                             aaaGAGAGTGTGCTCCCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATg                                   <  2:467888/151‑1 (MQ=255)                                                                                                              aaGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGc                                  <  2:160061/151‑1 (MQ=255)                                                                                                               agagatTGTGTTACCGGGTGGCGCCATTGAAGGGGGCGGGAACGATTCTGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCg                                         <  2:531717/143‑1 (MQ=255)                                                                                                                gagagTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCgg                                <  2:512114/151‑1 (MQ=255)                                                                                                                gagagTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCgg                                >  1:392389/1‑151 (MQ=255)                                                                                                                 agagTGTGCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCggg                               >  2:82795/1‑151 (MQ=255)                                                                                                                      gtgCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACgg                          <  2:524763/151‑1 (MQ=255)                                                                                                                      gagCTACCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCAGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGTCAATGCGGGAACgg                          >  2:531361/3‑151 (MQ=255)                                                                                                                           aCCGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAg                      <  2:576533/150‑1 (MQ=255)                                                                                                                             cGGGTGGCGCCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCg                    >  2:563227/1‑150 (MQ=255)                                                                                                                                    cgcCATTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCggg            >  2:234769/1‑151 (MQ=255)                                                                                                                                       cATGGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGaa         <  1:2049/151‑1 (MQ=255)                                                                                                                                        aTTGAAGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGa          >  2:62708/1‑149 (MQ=255)                                                                                                                                             aGGCGGCCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGAt      <  2:653840/148‑1 (MQ=255)                                                                                                                                              ggcggcCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCg  >  2:577565/1‑151 (MQ=255)                                                                                                                                              ggcggcCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCg  <  2:58848/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CCGCTGCTGTCGGTATTCGGCGGTAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCG  >  NC_000913/3563048‑3563339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑