Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,572,038	T→C	T489A (ACC→GCC)	glgB ←	1,4‑alpha‑glucan branching enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,572,038	0	T	C	100.0%	181.9 / NA	51	T489A (ACC→GCC)	glgB	1,4‑alpha‑glucan branching enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/25);  total (26/25)

CGGTCGAGAATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGTGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATT  >  NC_000913/3571890‑3572182                                                                                                                                                     |                                                                                                                                                 cGGTCGAGAATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCTAGATTCATGTAGTCCAGGgcgt                                                                                                                                                >  2:33743/1‑151 (MQ=255)   gTCGAGAATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGgcgt                                                                                                                                                >  1:404351/1‑149 (MQ=255)     cGAGAATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCAt                                                                                                                                             <  2:231935/150‑1 (MQ=255)     cGAGAATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATg                                                                                                                                            <  2:595286/151‑1 (MQ=255)       agaATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGc                                                                                                                                           >  2:142453/1‑150 (MQ=255)             gATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAg                                                                                                                                     <  2:391684/150‑1 (MQ=255)              aTTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAAAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGcc                                                                                                                                   >  1:555132/1‑151 (MQ=255)                  tttACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAgg                                                                                                                               >  2:516434/1‑151 (MQ=255)                        gTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCAc                                                                                                                         >  1:223824/1‑151 (MQ=255)                          ggACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACt                                                                                                                        <  1:45716/150‑1 (MQ=255)                              cACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTAc                                                                                                                   >  2:644572/1‑151 (MQ=255)                                  tcatcaTGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTAcc                                                                                                                  >  1:413022/1‑148 (MQ=255)                                   catcatGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGa                                                                                                               <  2:362466/150‑1 (MQ=255)                                      catGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGcc                                                                                                           >  2:291603/1‑151 (MQ=255)                                        tGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGccc                                                                                                          >  1:48060/1‑150 (MQ=255)                                           gACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGAc                                                                                                      <  2:481895/151‑1 (MQ=255)                                                   cAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCat                                                                                               >  1:295645/1‑150 (MQ=255)                                                     ggACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATc                                                                                            <  1:191962/151‑1 (MQ=255)                                                         gAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGc                                                                                        <  1:680249/151‑1 (MQ=255)                                                           aGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCgg                                                                                      <  2:174056/151‑1 (MQ=255)                                                             ttttCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGAc                                                                                    >  2:34185/1‑151 (MQ=255)                                                               ttCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACg                                                                                   <  2:596768/150‑1 (MQ=255)                                                                            tAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAgg                                                                       <  2:500775/149‑1 (MQ=255)                                                                                 aaTCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCg                                                                <  2:522021/151‑1 (MQ=255)                                                                                       gAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGAc                                                          >  2:439342/1‑151 (MQ=255)                                                                                        aaGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGAct                                                         <  1:566235/151‑1 (MQ=255)                                                                                        aaGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGAct                                                         >  2:562843/1‑151 (MQ=255)                                                                                              aGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGActcctc                                                     >  1:341322/1‑149 (MQ=255)                                                                                              aGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGActcctc                                                     >  2:44568/1‑149 (MQ=255)                                                                                               gTTTAGCGTGATGATACTGACCATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGc                                                  <  2:23695/151‑1 (MQ=255)                                                                                                   aTCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCAtt                                              >  2:1727/1‑151 (MQ=255)                                                                                                         atgatACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTcacc                                        <  2:133436/151‑1 (MQ=255)                                                                                                         atgatACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCAGAGGTTCTACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTcacc                                        >  2:633869/1‑151 (MQ=255)                                                                                                           gatACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTcaccg                                       <  1:90824/150‑1 (MQ=255)                                                                                                             tACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTcactgcac                                    >  2:146210/1‑151 (MQ=255)                                                                                                               cTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTcaccgcaccg                                  >  1:505185/1‑151 (MQ=255)                                                                                                                 gACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCgg                                 >  1:147895/1‑150 (MQ=255)                                                                                                                    gATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAAc                             >  2:305574/1‑151 (MQ=255)                                                                                                                       aaaCCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCt                           <  2:231643/150‑1 (MQ=255)                                                                                                                         aCCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCt                        >  1:171170/1‑151 (MQ=255)                                                                                                                            gggTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCAc                     <  1:432331/151‑1 (MQ=255)                                                                                                                            gggTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCAc                     <  2:287756/151‑1 (MQ=255)                                                                                                                                cGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCa                   >  2:731/1‑149 (MQ=255)                                                                                                                                  aGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGaa               <  2:479753/151‑1 (MQ=255)                                                                                                                                  aGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGaa               <  2:465293/151‑1 (MQ=255)                                                                                                                                      tCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATAc            <  1:594840/150‑1 (MQ=255)                                                                                                                                       cATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACgg          <  1:464394/151‑1 (MQ=255)                                                                                                                                       cATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACg           <  2:56847/150‑1 (MQ=255)                                                                                                                                         tGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGtt        >  1:103934/1‑151 (MQ=255)                                                                                                                                          gTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTg       <  1:150823/151‑1 (MQ=255)                                                                                                                                             gTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGt     >  1:11196/1‑150 (MQ=255)                                                                                                                                              tCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTAt   >  1:13267/1‑151 (MQ=255)                                                                                                                                               ccAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTAtt  >  1:633115/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CGGTCGAGAATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGTGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATT  >  NC_000913/3571890‑3572182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑