Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,572,259	T→C	Y415C (TAC→TGC)	glgB ←	1,4‑alpha‑glucan branching enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,572,259	0	T	C	100.0%	165.0 / NA	46	Y415C (TAC→TGC)	glgB	1,4‑alpha‑glucan branching enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/24);  total (22/24)

GGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCC  >  NC_000913/3572112‑3572399                                                                                                                                                    |                                                                                                                                             gggAAATCGGTAGACTCCTCAGCCATTGTCCCCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAg                                                                                                                                            <  2:27573/150‑1 (MQ=255)      aTCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTcgcg                                                                                                                                       >  1:185456/1‑150 (MQ=255)        cGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGAt                                                                                                                                     >  2:95965/1‑150 (MQ=255)        cGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGAt                                                                                                                                     <  1:238806/150‑1 (MQ=255)        cGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGAt                                                                                                                                     <  1:468152/150‑1 (MQ=255)         ggTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATa                                                                                                                                    >  2:306548/1‑150 (MQ=255)                     aGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGcc                                                                                                                       <  2:172838/151‑1 (MQ=255)                        cATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCAcc                                                                                                                    <  2:422305/151‑1 (MQ=255)                          ttGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCg                                                                                                                   <  2:105132/150‑1 (MQ=255)                           tGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGc                                                                                                                  <  2:251164/150‑1 (MQ=255)                               accgcaccgGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCg                                                                                                              >  1:17191/1‑150 (MQ=255)                                     ccgGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACgcgca                                                                                                       <  2:247065/151‑1 (MQ=255)                                          aaCCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACgcgcagcgc                                                                                                   >  1:366387/1‑150 (MQ=255)                                               gCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCa                                                                                               <  2:505172/149‑1 (MQ=255)                                                     cAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCaaa                                                                                       <  1:527467/151‑1 (MQ=255)                                                     cAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCaaa                                                                                       <  2:528254/151‑1 (MQ=255)                                                        gAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACg                                                                                    <  2:61069/151‑1 (MQ=255)                                                            aCGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTc                                                                                 <  2:633096/150‑1 (MQ=255)                                                                 tGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATcc                                                                            >  2:263772/1‑150 (MQ=255)                                                                  ggTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCa                                                                           <  2:251668/150‑1 (MQ=255)                                                                          gCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAgcgc                                                                  >  1:550209/1‑151 (MQ=255)                                                                               aaTTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTAc                                                             <  2:214722/151‑1 (MQ=255)                                                                                aTTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTAcc                                                            <  1:131182/151‑1 (MQ=255)                                                                                   cAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACcga                                                          <  1:291609/150‑1 (MQ=255)                                                                                       cGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAgg                                                     >  1:600835/1‑151 (MQ=255)                                                                                       cGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAgg                                                     >  2:190183/1‑151 (MQ=255)                                                                                       cGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAg                                                      <  1:586308/150‑1 (MQ=255)                                                                                         cTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGa                                                    >  1:387591/1‑150 (MQ=255)                                                                                           tCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGt                                                 >  2:428079/1‑151 (MQ=255)                                                                                            cAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGt                                                 <  1:395103/150‑1 (MQ=255)                                                                                                aTTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTg                                            >  1:319189/1‑151 (MQ=255)                                                                                                     cgcgCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACtt                                        <  1:338608/150‑1 (MQ=255)                                                                                                         cccgccAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACgg                                   >  1:186648/1‑151 (MQ=255)                                                                                                         cccgccAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACgg                                   <  2:404340/151‑1 (MQ=255)                                                                                                            gccAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGa                                <  2:417574/151‑1 (MQ=255)                                                                                                                    cgttcgGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGGCTTCACGGCGACCATAGtt                        >  2:674397/1‑151 (MQ=255)                                                                                                                    cgttcgGGATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGtt                        >  1:28924/1‑151 (MQ=255)                                                                                                                         gggATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGt                      >  2:334285/1‑148 (MQ=255)                                                                                                                          ggATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGAt                  <  1:644591/151‑1 (MQ=255)                                                                                                                          ggATCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGAt                  <  1:539023/151‑1 (MQ=255)                                                                                                                             tCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCCCCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATc                 >  2:299427/1‑149 (MQ=255)                                                                                                                             tCCACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATc                 >  1:402075/1‑149 (MQ=255)                                                                                                                               cACTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCg             >  1:442903/1‑151 (MQ=255)                                                                                                                                aCTCCCCCTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCgt            >  1:395515/1‑151 (MQ=255)                                                                                                                                     cccTCTTTACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTcc       >  2:432217/1‑151 (MQ=255)                                                                                                                                          tttACGGCTGCAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTcc  >  2:85494/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                             GGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATTACGCAAGAATTCAATCGCTTCAAGATTCTCGCGCCCGCCAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCC  >  NC_000913/3572112‑3572399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑