Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,575,146	T→C	S26P (TCC→CCC)	yhgN →	UPF0056 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,575,146	0	T	C	100.0%	137.1 / NA	37	S26P (TCC→CCC)	yhgN	UPF0056 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/17);  total (20/17)

TCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCAT  >  NC_000913/3575001‑3575291                                                                                                                                                  |                                                                                                                                                  tCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGt                                                                                                                                               >  1:88505/1‑150 (MQ=255)  cAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTAc                                                                                                                                             >  2:286049/1‑151 (MQ=255)    gTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTg                                                                                                                                           <  2:201882/151‑1 (MQ=255)    gTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTg                                                                                                                                           <  1:574797/151‑1 (MQ=255)      aaTGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGTTTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGa                                                                                                                                          >  2:209329/1‑150 (MQ=255)       aTGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGaa                                                                                                                                         >  2:568602/1‑150 (MQ=255)           gACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACAt                                                                                                                                     >  1:427559/1‑150 (MQ=255)                aTCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGa                                                                                                                                <  2:318203/150‑1 (MQ=255)                     gCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGaa                                                                                                                          <  1:96587/151‑1 (MQ=255)                        ttGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGaaa                                                                                                                         >  2:285739/1‑149 (MQ=255)                          gTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGAc                                                                                                                     <  1:622116/151‑1 (MQ=255)                                    aGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAAt                                                                                                           >  1:63960/1‑151 (MQ=255)                                            cgcgGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGc                                                                                                   <  2:276238/151‑1 (MQ=255)                                                gCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCg                                                                                                  >  1:409271/1‑148 (MQ=255)                                                              aTTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGctc                                                                                 >  1:61867/1‑151 (MQ=255)                                                                  gAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCt                                                                             <  1:650363/151‑1 (MQ=255)                                                                  gAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCt                                                                             <  2:635011/151‑1 (MQ=255)                                                                  gAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGACCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCt                                                                             <  1:495698/151‑1 (MQ=255)                                                                   aGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTg                                                                            <  1:120230/151‑1 (MQ=255)                                                                      tATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGTGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTg                                                                         >  1:516869/1‑151 (MQ=255)                                                                             gaaATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTgg                                                                  >  2:670050/1‑151 (MQ=255)                                                                                     ttCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCt                                                           <  1:253831/150‑1 (MQ=255)                                                                                     ttCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTg                                                          >  2:550064/1‑151 (MQ=255)                                                                                      tCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGt                                                         >  2:143239/1‑151 (MQ=255)                                                                                           agcagTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCg                                                    >  1:149819/1‑151 (MQ=255)                                                                                             cagTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCggg                                                  <  1:47931/151‑1 (MQ=255)                                                                                                ttttATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTg                                                >  1:582168/1‑150 (MQ=255)                                                                                                    aTTGATCCTGATTATGGATCCGCTAGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGaaa                                           <  1:6329/151‑1 (MQ=255)                                                                                                        aTCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTc                                       >  2:104538/1‑151 (MQ=255)                                                                                                          ccTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTg                                     >  2:92279/1‑151 (MQ=255)                                                                                                                tATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCAttt                               >  2:16473/1‑151 (MQ=255)                                                                                                                             ggAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAg                  <  2:531028/151‑1 (MQ=255)                                                                                                                               aaaCCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGc                 >  1:215338/1‑150 (MQ=255)                                                                                                                                         aTTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGtc       <  2:225047/150‑1 (MQ=255)                                                                                                                                         aTTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGt        <  2:304622/149‑1 (MQ=255)                                                                                                                                            ttCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTcc    >  2:539829/1‑150 (MQ=255)                                                                                                                                              cATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCAt  <  1:188289/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  TCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCAT  >  NC_000913/3575001‑3575291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑