Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,689,711	G→A	P184L (CCG→CTG)	bcsZ ←	endo‑1,4‑D‑glucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,689,711	0	G	A	100.0%	169.0 / NA	45	P184L (CCG→CTG)	bcsZ	endo‑1,4‑D‑glucanase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/28);  total (17/28)

AGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAA  >  NC_000913/3689567‑3689854                                                                                                                                                 |                                                                                                                                                aGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAgg                                                                                                                                            <  2:501428/150‑1 (MQ=255)  gCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTa                                                                                                                                          <  2:374904/151‑1 (MQ=255)   cTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGt                                                                                                                                           >  2:415713/1‑149 (MQ=255)     gCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGc                                                                                                                                        >  1:126193/1‑150 (MQ=255)       cAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTgg                                                                                                                                     <  2:493571/151‑1 (MQ=255)            tttGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGtt                                                                                                                                 >  1:327037/1‑150 (MQ=255)                 cTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGCTTCCAGAAATAAACGTTGATTGGTTTCACACAGCGTGGACCACGGCGCGCCAAAGCGGGCGTAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAAc                                                                                                                            >  1:585970/1‑150 (MQ=255)                    tctcATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGATTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGcc                                                                                                                         >  1:552824/1‑150 (MQ=255)                     ctcATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGcc                                                                                                                         >  2:468762/1‑149 (MQ=255)                        aTAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTg                                                                                                                    <  1:51518/151‑1 (MQ=255)                           gcgcACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTa                                                                                                                 <  1:516773/151‑1 (MQ=255)                               aCCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATcc                                                                                                              >  2:472998/1‑150 (MQ=255)                               aCCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGGTTCGCGCAGCGTGGTGCACGGCCCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACCCCAGCTGTTATcc                                                                                                              >  1:358798/1‑150 (MQ=255)                                cccAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTc                                                                                                            <  1:482574/151‑1 (MQ=255)                                 ccAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCa                                                                                                           <  2:660931/151‑1 (MQ=255)                                       tGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCaaa                                                                                                      >  2:97516/1‑150 (MQ=255)                                                          gCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTaaca                                                                                  <  1:73080/151‑1 (MQ=255)                                                               ttCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATg                                                                             >  2:157284/1‑151 (MQ=255)                                                                tCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATg                                                                             <  1:573476/150‑1 (MQ=255)                                                                tCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATg                                                                             <  1:324238/150‑1 (MQ=255)                                                                      aaTAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAAccc                                                                       >  1:316308/1‑150 (MQ=255)                                                                         aaaCGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAAcccagcc                                                                   <  2:474602/151‑1 (MQ=255)                                                                         aaaCGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAAcccagc                                                                    <  2:197812/150‑1 (MQ=255)                                                                               tGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGCGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGc                                                              >  1:623155/1‑150 (MQ=255)                                                                                 aTTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCAcc                                                           <  2:485609/151‑1 (MQ=255)                                                                                           cgcAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACtt                                                 <  2:377009/151‑1 (MQ=255)                                                                                            gcAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACtt                                                 <  2:140472/150‑1 (MQ=255)                                                                                              aGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTtcct                                              >  1:25227/1‑151 (MQ=255)                                                                                              aGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTtcc                                               <  1:395755/150‑1 (MQ=255)                                                                                                 gTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTccc                                           <  2:497553/151‑1 (MQ=255)                                                                                                   ggTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCcgc                                         <  1:406106/151‑1 (MQ=255)                                                                                                      ccACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCcgcgc                                       <  1:464009/150‑1 (MQ=255)                                                                                                        aCGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGAt                                    <  2:99335/151‑1 (MQ=255)                                                                                                         cGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGAt                                    >  1:553338/1‑150 (MQ=255)                                                                                                         cGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATa                                   <  2:224115/151‑1 (MQ=255)                                                                                                            cgcgcCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGt                                <  1:159683/151‑1 (MQ=255)                                                                                                                 cAAAGCGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGtttt                             >  1:29117/1‑149 (MQ=255)                                                                                                                      cGGGTGAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAAcg                      >  1:395625/1‑151 (MQ=255)                                                                                                                           gAAATACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCt                  <  1:638816/150‑1 (MQ=255)                                                                                                                              aTACTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCg              <  2:673533/151‑1 (MQ=255)                                                                                                                                 cTGCGCCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCg              >  2:501182/1‑148 (MQ=255)                                                                                                                                   gcgcCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCATCAAAACCCTCTTTGCCCGGTATCAACATGGAACCCAGCCCAGGCCCCGTCACCTCTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTc         <  2:527723/151‑1 (MQ=255)                                                                                                                                     gcCAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCgg       <  1:161927/151‑1 (MQ=255)                                                                                                                                      ccAGCGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGt      <  2:633215/151‑1 (MQ=255)                                                                                                                                          cGTCGGCAGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATaa  <  1:66656/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                AGCTGCCAGCCTTTGTCTTTCTCATAGCGCACCCAGTCTGGCGAAAAACCTTTCGGGGCGGTTTCCAGCAATAAACGTTGATTGGTTTCGCGCAGCGTGGTCCACGGCGCGCCAAAGCGGGTGAAATACTGCGCCAGCGTCGGCGGCAGGTAGCTGGGGTTAAAACGCCAGCTGTTATCCTCAGCAAAACCCACTTTGCCCGGTAACAACATGGAACCCAGCCCAGGCACCGTCACCACTTCCTCCCGCGCGATACGTTTTAGCAACGCGCTGCCGATGTCGGTATAA  >  NC_000913/3689567‑3689854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑