Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,700,370	A→G	intergenic (‑283/‑193)	ldrD ← / → yhjV	toxic polypeptide, small/putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,700,370	0	A	G	100.0%	163.1 / NA	45	intergenic (‑283/‑193)	ldrD/yhjV	toxic polypeptide, small/putative transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/18);  total (27/18)

CAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGAACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGG  >  NC_000913/3700223‑3700517                                                                                                                                                    |                                                                                                                                                    caGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGCACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTTTTTGCAATAGTTGCGGt                                                                                                                                                   >  2:11109/1‑150 (MQ=255)          aGCCCCCGCAACATTGCGCCTCACCGGAACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCgg                                                                                                                                         <  1:133109/151‑1 (MQ=255)           gCCCCCGCAACATTGCGCCTCACCGGAACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCggg                                                                                                                                        >  1:283722/1‑151 (MQ=255)              cccGCAACATTGCGCCTCACCGGAACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTg                                                                                                                                     >  2:425422/1‑151 (MQ=255)               ccGCAACATTGCGCCTCACCGGAACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTg                                                                                                                                     >  1:199459/1‑150 (MQ=255)                    aCATTGCGCCTCACCGGAACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTcc                                                                                                                                >  2:195469/1‑150 (MQ=255)                      aTTGCGCCTCACCGGAACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAg                                                                                                                              <  2:295790/150‑1 (MQ=255)                         gcgcCTCACCGGAACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTg                                                                                                                          >  1:337952/1‑151 (MQ=255)                          cgcCTCACCGGAACCTTTCCGGCTTGCCGATGATTCTACGGTTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTgg                                                                                                                         >  2:606458/1‑151 (MQ=255)                           gcCTCACCGGAACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGt                                                                                                                        <  2:664976/151‑1 (MQ=255)                             cTCACCGGAACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGt                                                                                                                        >  1:254460/1‑149 (MQ=255)                                   ggAACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAAt                                                                                                                >  2:343160/1‑151 (MQ=255)                                   ggAACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAAt                                                                                                                <  2:160221/151‑1 (MQ=255)                                             cGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAAtttt                                                                                                      <  2:56412/151‑1 (MQ=255)                                                   gCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGa                                                                                                 >  1:129987/1‑150 (MQ=255)                                                        tGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTgcg                                                                                           >  2:111987/1‑151 (MQ=255)                                                                  aTTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTcgcg                                                                                 <  1:198051/151‑1 (MQ=255)                                                                           tACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTcccc                                                                         <  1:314089/150‑1 (MQ=255)                                                                            aCTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAg                                                                       <  1:454900/151‑1 (MQ=255)                                                                                tCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGGGa                                                                    >  1:13463/1‑150 (MQ=255)                                                                                 cAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAAt                                                                  >  2:250269/1‑151 (MQ=255)                                                                                    cACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTg                                                               >  2:289034/1‑151 (MQ=255)                                                                                            aaTCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAGTCTGTTGCCTc                                                        >  2:177332/1‑150 (MQ=255)                                                                                             aTCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAt                                                      >  1:57874/1‑151 (MQ=255)                                                                                                 tCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAttt                                                    <  1:339675/149‑1 (MQ=255)                                                                                                 tCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAt                                                      <  2:368017/147‑1 (MQ=255)                                                                                                    tCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTAc                                               >  2:280335/1‑151 (MQ=255)                                                                                                            cgATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCg                                       >  2:601961/1‑151 (MQ=255)                                                                                                             gATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGt                                      <  1:104159/151‑1 (MQ=255)                                                                                                                    tCCAGCTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGAc                               >  2:510953/1‑151 (MQ=255)                                                                                                                         cTTAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTtctc                          >  2:505342/1‑151 (MQ=255)                                                                                                                          ttAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTcc                         >  2:267568/1‑151 (MQ=255)                                                                                                                           tAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTtctc                          <  1:624382/149‑1 (MQ=255)                                                                                                                           tAATTTCTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTcc                         >  2:297177/1‑150 (MQ=255)                                                                                                                                 cTTTATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGtt                  >  1:347930/1‑151 (MQ=255)                                                                                                                                   ttATTTGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTgc                >  1:217452/1‑151 (MQ=255)                                                                                                                                        tGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTg                                                            <  2:174729/102‑1 (MQ=255)                                                                                                                                        tGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTg                                                            >  1:174734/1‑102 (MQ=255)                                                                                                                                        tGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCaa            <  2:446035/150‑1 (MQ=255)                                                                                                                                        tGCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCaa            <  1:148650/150‑1 (MQ=255)                                                                                                                                         gCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCa             >  2:299908/1‑148 (MQ=255)                                                                                                                                         gCAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAaga          <  2:3529/151‑1 (MQ=255)                                                                                                                                          cAATAGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAagag         <  1:242489/151‑1 (MQ=255)                                                                                                                                              aGTTGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAAc     <  1:415673/151‑1 (MQ=255)                                                                                                                                                 tGCGGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTgg  >  1:480163/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGAACCTTTCCGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGG  >  NC_000913/3700223‑3700517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑