Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,843,430	C→T	W120* (TGG→TGA)	adeQ ←	adenine permease, high affinity; adenine:H+ symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,843,430	0	C	T	100.0%	149.1 / NA	39	W120* (TGG→TGA)	adeQ	adenine permease, high affinity; adenine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/25);  total (14/25)

CGACAATAACGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAA  >  NC_000913/3843282‑3843560                                                                                                                                                     |                                                                                                                                   cGACAATAACGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCa                                                                                                                                  <  2:611023/151‑1 (MQ=255)  gACAATAACGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAg                                                                                                                                 <  1:383591/151‑1 (MQ=255)     aaTAACGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAat                                                                                                                              <  2:97907/151‑1 (MQ=255)        aaCGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAatat                                                                                                                            <  1:615350/150‑1 (MQ=255)          cGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTgc                                                                                                                         <  2:466099/151‑1 (MQ=255)          cGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCCGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTgc                                                                                                                         >  2:554077/1‑151 (MQ=255)               gTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGccc                                                                                                                     >  2:33350/1‑150 (MQ=255)                 aTTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATc                                                                                                                  >  2:548628/1‑151 (MQ=255)                     tttAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCg                                                                                                               <  1:582229/150‑1 (MQ=255)                      ttAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGt                                                                                                             >  1:95480/1‑151 (MQ=255)                       tAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTc                                                                                                            >  1:360468/1‑151 (MQ=255)                           cccATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGcc                                                                                                        >  2:428110/1‑151 (MQ=255)                                 aaGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGgaga                                                                                                  <  2:427723/151‑1 (MQ=255)                                     cGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGcc                                                                                              >  2:208567/1‑151 (MQ=255)                                      gATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGccc                                                                                             >  2:645182/1‑151 (MQ=255)                                          aataatCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCg                                                                                         >  1:482452/1‑151 (MQ=255)                                                  aaTTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCccacg                                                                                 <  2:100951/151‑1 (MQ=255)                                                        gCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCccacgaccacg                                                                           >  1:219543/1‑151 (MQ=255)                                                           ggTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCccacgaccacga                                                                          >  1:422304/1‑149 (MQ=255)                                                              gATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCa                                                                     <  1:406731/151‑1 (MQ=255)                                                               aTACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCaa                                                                    <  2:95478/151‑1 (MQ=255)                                                                    aaTACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAaagaag                                                               <  2:536190/151‑1 (MQ=255)                                                                    aaTACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAaagaag                                                               <  1:54363/151‑1 (MQ=255)                                                                     aTACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAaagaag                                                               >  1:292356/1‑150 (MQ=255)                                                                        cGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGt                                                           <  1:453466/151‑1 (MQ=255)                                                                            aaCTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAg                                                       <  2:528831/151‑1 (MQ=255)                                                                             aCTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGc                                                      <  2:669297/151‑1 (MQ=255)                                                                             aCTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGc                                                      <  2:6954/151‑1 (MQ=255)                                                                              cTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGcc                                                     >  2:372276/1‑151 (MQ=255)                                                                                     gAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGc                                              >  1:664712/1‑151 (MQ=255)                                                                                             gagaTCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCa                                      <  1:462374/151‑1 (MQ=255)                                                                                                        gTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCAACGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGt                           <  1:433487/151‑1 (MQ=255)                                                                                                          aCCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTaa                         <  2:173878/151‑1 (MQ=255)                                                                                                           ccGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAg                        <  1:330793/151‑1 (MQ=255)                                                                                                               aTACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAg                    <  2:68006/151‑1 (MQ=255)                                                                                                                 aCGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCa                  <  1:441113/151‑1 (MQ=255)                                                                                                                              aGCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCg     <  2:592435/151‑1 (MQ=255)                                                                                                                               gCAAAAATAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGc    <  2:300735/151‑1 (MQ=255)                                                                                                                                 aaaaaTAGTCCAACTGCGCCTCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCaa  <  1:8946/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                   CGACAATAACGCCAGTATTTTTTAATCCCATTAAGGCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAA  >  NC_000913/3843282‑3843560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑