Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,928,167	T→C	R479R (AGA→AGG)	viaA ←	stimulator of RavA ATPase activity; von Willebrand factor domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,928,167	0	T	C	100.0%	144.9 / NA	41	R479R (AGA→AGG)	viaA	stimulator of RavA ATPase activity; von Willebrand factor domain protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/15);  total (26/15)

CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTAC  >  NC_000913/3928024‑3928306                                                                                                                                                |                                                                                                                                            cATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGc                                                                                                                                       >  2:597641/1‑150 (MQ=255)    cggcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAggc                                                                                                                                   >  1:27824/1‑151 (MQ=255)    cggcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAgg                                                                                                                                    >  2:667651/1‑150 (MQ=255)     ggcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAggcg                                                                                                                                  >  2:371055/1‑151 (MQ=255)       cggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAggcggc                                                                                                                                <  1:561530/151‑1 (MQ=255)        ggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAggcggc                                                                                                                                >  2:550392/1‑150 (MQ=255)            gTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGc                                                                                                                           >  2:640426/1‑151 (MQ=255)              aTCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCAt                                                                                                                         >  2:90025/1‑151 (MQ=255)              aTCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCAt                                                                                                                         >  2:677943/1‑151 (MQ=255)                    cAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGt                                                                                                                   <  1:368689/151‑1 (MQ=255)                      gTCTCGTTTGGCTATGCGTCTGCTGCAACTGCAGCATATCGGGCAGGATCAGTGTGGAGTATGGCCAGGTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATATATCGTCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTa                                                                                                                  <  2:507873/150‑1 (MQ=255)                        ctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATc                                                                                                                >  1:14575/1‑150 (MQ=255)                           gTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCaa                                                                                                              >  1:382238/1‑149 (MQ=255)                              tGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcg                                                                                                          <  1:405882/150‑1 (MQ=255)                              tGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAACAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcg                                                                                                          <  1:36761/150‑1 (MQ=255)                                          tgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCg                                                                                             >  2:174247/1‑151 (MQ=255)                                             tgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGaa                                                                                           >  1:519529/1‑150 (MQ=255)                                              gcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGaaa                                                                                          <  1:506109/150‑1 (MQ=255)                                              gcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGaa                                                                                           <  2:400563/149‑1 (MQ=255)                                                 aCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATgc                                                                                      >  1:7805/1‑151 (MQ=255)                                                      cGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCatg                                                                                 <  1:227174/151‑1 (MQ=255)                                                              ggCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCggg                                                                         >  2:404264/1‑151 (MQ=255)                                                                 cAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGttt                                                                      >  1:102704/1‑151 (MQ=255)                                                                    gTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGcc                                                                   >  2:163571/1‑151 (MQ=255)                                                                       cAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGTCgt                                                                 <  2:340403/150‑1 (MQ=255)                                                                        aGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCgtgt                                                               >  1:162534/1‑151 (MQ=255)                                                                                         gctgctGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGc                                              >  1:74005/1‑151 (MQ=255)                                                                                                  cgcgAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCg                                     <  1:172883/151‑1 (MQ=255)                                                                                                   gcgAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGa                                    <  1:2694/151‑1 (MQ=255)                                                                                                     gagaGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATg                                  >  2:678869/1‑151 (MQ=255)                                                                                                          cGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGAt                             >  2:90615/1‑151 (MQ=255)                                                                                                           gTCCCTTCTCTGCTGTAATAATTTATCTCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATg                            <  2:207853/151‑1 (MQ=255)                                                                                                                 tctcTGCTGTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTAccc                       >  1:578432/1‑150 (MQ=255)                                                                                                                      gctgTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCt                    <  1:625865/148‑1 (MQ=255)                                                                                                                      gctgTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCa                 <  2:266739/151‑1 (MQ=255)                                                                                                                       ctgTAATAATTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGc                  >  1:403496/1‑149 (MQ=255)                                                                                                                              aatTTATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTc         <  2:633809/151‑1 (MQ=255)                                                                                                                                  tATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACtt     >  1:83853/1‑151 (MQ=255)                                                                                                                                  tATCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACtt     >  1:100406/1‑151 (MQ=255)                                                                                                                                    tCGCCGCCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACttt    >  2:636643/1‑150 (MQ=255)                                                                                                                                     cgccgcCAGCGCCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTAc  >  1:491654/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                            CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGCCAGATATGATCGAAAATGCGCATGATGCCGGGTTTGCCGTGTGCCGACATCGCCACGGCATGAAAGCGATGCTGATGTACCCGCTGCAGCTCTTTCACTTTAC  >  NC_000913/3928024‑3928306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑