Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,979,674	T→C	G358G (GGT→GGC)	wzyE →	putative ECA polysaccharide chain elongation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,979,674	0	T	C	100.0%	159.2 / NA	44	G358G (GGT→GGC)	wzyE	putative ECA polysaccharide chain elongation protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/19);  total (25/19)

GCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGTCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTC  >  NC_000913/3979526‑3979820                                                                                                                                                     |                                                                                                                                                   gCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCt                                                                                                                                                  >  1:89429/1‑151 (MQ=255)   ccGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGGGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTg                                                                                                                                                 >  2:476116/1‑150 (MQ=255)    cGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGat                                                                                                                                               >  1:66283/1‑151 (MQ=255)    cGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGat                                                                                                                                               <  1:61171/151‑1 (MQ=255)       gTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGatca                                                                                                                                             <  2:672152/150‑1 (MQ=255)            gTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAAt                                                                                                                                       <  1:538575/151‑1 (MQ=255)                  aaCTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCg                                                                                                                                 >  2:676593/1‑151 (MQ=255)                      cAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTg                                                                                                                             >  1:102535/1‑151 (MQ=255)                      cAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTg                                                                                                                             <  1:616568/151‑1 (MQ=255)                              tactTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATg                                                                                                                     >  1:338790/1‑151 (MQ=255)                                ctTTCCCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTAGGa                                                                                                                    <  1:170141/150‑1 (MQ=255)                                ctTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATg                                                                                                                     >  1:651732/1‑149 (MQ=255)                                    aCCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTgg                                                                                                               >  2:413864/1‑151 (MQ=255)                                     ccTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTggg                                                                                                              <  1:453208/151‑1 (MQ=255)                                       tGGGCAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGc                                                                                                             >  1:604367/1‑150 (MQ=255)                                           aaGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACcg                                                                                                        >  2:235424/1‑151 (MQ=255)                                             gTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACcgcg                                                                                                      >  1:508204/1‑151 (MQ=255)                                               gCTGAATAACCGCTCCGGGCTGGCGATCTCGCCTACGATTATAGGCTTGCTGGTGGTGATGGGCGGAGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCCTCAAATGGTGCGACTGGCTGTATGAGCTGGGCAGCCGCGa                                                                                                     <  2:604678/150‑1 (MQ=255)                                               gCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGa                                                                                                     <  2:548464/150‑1 (MQ=255)                                                         cACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCt                                                                                          >  2:438507/1‑151 (MQ=255)                                                           cTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCtat                                                                                        >  1:137136/1‑151 (MQ=255)                                                            tCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCtat                                                                                        >  1:481009/1‑150 (MQ=255)                                                                 aCTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAg                                                                                    >  2:559151/1‑149 (MQ=255)                                                                     gCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCg                                                                               >  2:350126/1‑150 (MQ=255)                                                                     gCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGa                                                                              <  1:595646/151‑1 (MQ=255)                                                                           tcGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGc                                                                        >  2:257927/1‑151 (MQ=255)                                                                            cGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGat                                                                             <  2:420798/145‑1 (MQ=255)                                                                            cGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATAtt                                                                          <  1:343942/148‑1 (MQ=255)                                                                            cGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGca                                                                       <  2:487261/151‑1 (MQ=255)                                                                             gCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGca                                                                       <  2:346554/150‑1 (MQ=255)                                                                                       ataGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGc                                                             <  1:577998/150‑1 (MQ=255)                                                                                         aGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCttt                                                          >  2:242257/1‑151 (MQ=255)                                                                                             tCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTgggg                                                      >  2:586327/1‑151 (MQ=255)                                                                                              cACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTggg                                                       <  2:371786/149‑1 (MQ=255)                                                                                                  ggtggtGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATc                                                 <  1:668004/151‑1 (MQ=255)                                                                                                   gtggtgATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCt                                                >  1:88280/1‑151 (MQ=255)                                                                                                      gtgATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCa                                             <  1:54879/151‑1 (MQ=255)                                                                                                       tgATGGGCGTCGCCTTGTTCATCCCGCTCGTGGCGATCGTGGTTGGCCTGATCATCAAATTGTTCTACTGGCTGTATGAGCTGGGCAACCGCGAGCTTAATCGCTGTAAATCTGCGATATTGCGCAGTTTCTGCTTTGGGGCGATcct                                               <  2:593448/148‑3 (MQ=255)                                                                                                             gcggcgCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGAt                                      <  2:400332/151‑1 (MQ=255)                                                                                                                  gcgTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGc                                 >  2:35073/1‑151 (MQ=255)                                                                                                                     ttgttCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTgg                              >  2:670894/1‑151 (MQ=255)                                                                                                                        ttCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGgcgc                           >  2:240349/1‑151 (MQ=255)                                                                                                                         tCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGcgcg                          >  1:675349/1‑151 (MQ=255)                                                                                                                                         gATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCg          >  1:367372/1‑151 (MQ=255)                                                                                                                                              tggttggcCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTTGt     >  1:89725/1‑151 (MQ=255)                                                                                                                                                  tggcCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTTGtctc  >  2:534129/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGTCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTC  >  NC_000913/3979526‑3979820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑