Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,114,622	A→G	S320P (TCC→CCC)	glpX ←	fructose 1,6‑bisphosphatase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,114,622	0	A	G	100.0%	192.1 / NA	50	S320P (TCC→CCC)	glpX	fructose 1,6‑bisphosphatase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/30);  total (20/30)

TTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGC  >  NC_000913/4114474‑4114761                                                                                                                                                     |                                                                                                                                            tttttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCt                                                                                                                                           >  1:143393/1‑151 (MQ=255)     tctcCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAAt                                                                                                                                       >  2:459763/1‑151 (MQ=255)           gTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATgcggcg                                                                                                                                 <  1:552964/151‑1 (MQ=255)           gTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATgcggc                                                                                                                                  <  1:223208/150‑1 (MQ=255)           gTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATgcggc                                                                                                                                  <  1:489647/150‑1 (MQ=255)                      cTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTg                                                                                                                      >  1:337253/1‑151 (MQ=255)                      cTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTg                                                                                                                      >  1:636204/1‑151 (MQ=255)                        tGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTg                                                                                                                      <  2:481542/149‑1 (MQ=255)                          ccTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACtt                                                                                                                  <  2:126641/151‑1 (MQ=255)                           cTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTg                                                                                                                 >  2:560969/1‑151 (MQ=255)                              aTCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCcg                                                                                                              >  2:389626/1‑151 (MQ=255)                               tCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCcgc                                                                                                             >  2:370633/1‑151 (MQ=255)                               tCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCcgc                                                                                                             >  1:74349/1‑151 (MQ=255)                                      tctcCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATc                                                                                                        >  1:201169/1‑149 (MQ=255)                                       ctcCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagc                                                                                                     >  2:151112/1‑151 (MQ=255)                                               aaaaaaaTTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTc                                                                                             >  1:132055/1‑151 (MQ=255)                                                       tCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTc                                                                                      <  2:56790/150‑1 (MQ=255)                                                        ccAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTcgc                                                                                    <  1:541826/151‑1 (MQ=255)                                                        ccAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTcgc                                                                                    <  1:641326/151‑1 (MQ=255)                                                          aGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGa                                                                                  <  2:193990/151‑1 (MQ=255)                                                          aGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGa                                                                                  <  2:110564/151‑1 (MQ=255)                                                           gTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGat                                                                                 <  1:630935/151‑1 (MQ=255)                                                               cGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTg                                                                             <  1:47346/151‑1 (MQ=255)                                                                   ggACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCtt                                                                         >  2:623181/1‑151 (MQ=255)                                                                    gACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCttt                                                                        <  2:126972/151‑1 (MQ=255)                                                                    gACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCttt                                                                        <  2:197677/151‑1 (MQ=255)                                                                       tGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCg                                                                     >  2:263788/1‑151 (MQ=255)                                                                       tGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCg                                                                     >  2:282262/1‑151 (MQ=255)                                                                            ggCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTa                                                                 >  2:646585/1‑150 (MQ=255)                                                                                 aaTCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCt                                                           <  2:412665/151‑1 (MQ=255)                                                                                      atcaaatcaaTCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTcc                                                        >  1:59854/1‑149 (MQ=255)                                                                                      atcaaatcaaTCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcag                                                      <  1:343091/151‑1 (MQ=255)                                                                                        caaatcaaTCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcagc                                                     <  2:120991/150‑1 (MQ=255)                                                                                             caatcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATc                                                <  2:608666/150‑1 (MQ=255)                                                                                              aatcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGc                                              >  1:168965/1‑151 (MQ=255)                                                                                              aatcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGc                                              >  2:623011/1‑151 (MQ=255)                                                                                                  agagGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCttt                                          <  1:352854/151‑1 (MQ=255)                                                                                                      gATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTa                                      <  2:554583/151‑1 (MQ=255)                                                                                                           gCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATAcc                                 <  1:605451/151‑1 (MQ=255)                                                                                                                 gCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTg                             <  1:291571/149‑1 (MQ=255)                                                                                                                   aTTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCag                         <  1:562845/151‑1 (MQ=255)                                                                                                                     ttCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCaga                        <  2:671714/150‑1 (MQ=255)                                                                                                                      tCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCaga                        <  2:435329/149‑1 (MQ=255)                                                                                                                        cGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGagaag                    <  1:505432/151‑1 (MQ=255)                                                                                                                            tCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATg                 >  2:125846/1‑150 (MQ=255)                                                                                                                               ttGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGAc               <  1:11351/149‑1 (MQ=255)                                                                                                                                tGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGa                <  2:604193/147‑1 (MQ=255)                                                                                                                                 gCGGTCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGGTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGa                <  2:615255/146‑1 (MQ=255)                                                                                                                                  cGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTAt          >  1:612508/1‑151 (MQ=255)                                                                                                                                          aTAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTgcgc  <  1:618357/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                            TTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGC  >  NC_000913/4114474‑4114761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑