Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,157,132	A→G	K95E (AAA→GAA)	argH →	argininosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,157,132	0	A	G	100.0%	171.7 / NA	47	K95E (AAA→GAA)	argH	argininosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/21);  total (26/21)

AGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGG  >  NC_000913/4156990‑4157270                                                                                                                                               |                                                                                                                                           aGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTggg                                                                                                                                     <  1:448820/150‑1 (MQ=255)     gtgtTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGt                                                                                                                                >  1:40206/1‑151 (MQ=255)       gtTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGtt                                                                                                                               <  1:159041/150‑1 (MQ=255)          aaCCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAgg                                                                                                                            <  1:543173/150‑1 (MQ=255)           aCCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCa                                                                                                                          <  1:240748/151‑1 (MQ=255)             cGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCaa                                                                                                                         >  2:434601/1‑150 (MQ=255)                agaagaGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCaaaa                                                                                                                       >  1:454984/1‑149 (MQ=255)                agaagaGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCaaaa                                                                                                                       >  1:439823/1‑149 (MQ=255)                               aCTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGt                                                                                                      <  1:5494/151‑1 (MQ=255)                                   gAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGcc                                                                                                  >  2:280716/1‑151 (MQ=255)                                         gcgcTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATg                                                                                            >  1:621438/1‑151 (MQ=255)                                         gcgcTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATg                                                                                            >  1:251398/1‑151 (MQ=255)                                              gAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCa                                                                                        <  2:616805/150‑1 (MQ=255)                                               aaCGTGATGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATg                                                                                            >  2:94233/1‑145 (MQ=255)                                                aCGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAgg                                                                                      >  2:205432/1‑150 (MQ=255)                                                     ttGGTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGACAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCg                                                                                 <  1:210726/150‑1 (MQ=255)                                                      tGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGAc                                                                               >  1:466804/1‑151 (MQ=255)                                                      tGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGAc                                                                               >  2:57233/1‑151 (MQ=255)                                                          ggAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGAc                                                                           >  1:68993/1‑151 (MQ=255)                                                                   tCGCGCCAGTCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACt                                                                   <  2:45439/150‑1 (MQ=255)                                                                   tCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACt                                                                   <  1:347892/150‑1 (MQ=255)                                                                      cgcCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTgtgg                                                               <  1:334930/151‑1 (MQ=255)                                                                        ccAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTgtggtg                                                             <  1:213122/151‑1 (MQ=255)                                                                          aGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGc                                                            >  1:205655/1‑150 (MQ=255)                                                                             ccACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCTACTGACCTGAAACTGTGGTGCAAAg                                                        <  2:187590/151‑1 (MQ=255)                                                                                 aacaaATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATAc                                                    >  2:338540/1‑151 (MQ=255)                                                                                  acaaATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATAcc                                                   >  2:487771/1‑151 (MQ=255)                                                                                      aTCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGtt                                                >  2:513211/1‑150 (MQ=255)                                                                                      aTCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTa                                               <  2:395921/151‑1 (MQ=255)                                                                                        ccTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAg                                              <  2:89234/150‑1 (MQ=255)                                                                                        ccTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGc                                             >  1:206663/1‑151 (MQ=255)                                                                                        ccTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGc                                             >  1:286185/1‑151 (MQ=255)                                                                                                     gCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACgg                                >  1:484957/1‑151 (MQ=255)                                                                                                     gCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACgg                                <  1:518455/151‑1 (MQ=255)                                                                                                     gCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACgg                                >  1:593667/1‑151 (MQ=255)                                                                                                            atatCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCg                         >  1:167395/1‑151 (MQ=255)                                                                                                            atatCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCg                         <  1:419637/151‑1 (MQ=255)                                                                                                             tatCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCgg                        >  1:577391/1‑151 (MQ=255)                                                                                                              atCCATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGGTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCgg                        <  1:217905/150‑1 (MQ=255)                                                                                                                ccATAGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCa                      <  2:11899/150‑1 (MQ=255)                                                                                                                    aGCTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGc                 >  2:118318/1‑151 (MQ=255)                                                                                                                      cTGGGTGGAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCa                >  2:293448/1‑150 (MQ=255)                                                                                                                            ggAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGgcg         <  2:370944/151‑1 (MQ=255)                                                                                                                            ggAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGgc          <  1:528863/150‑1 (MQ=255)                                                                                                                            ggAAGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGgc          <  2:80555/150‑1 (MQ=255)                                                                                                                              aaGGCAAACTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCt       >  2:190487/1‑151 (MQ=255)                                                                                                                                    aaCTGATCGACGAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCtggtgg  >  1:211373/1‑150 (MQ=255)                                                                                                                                               |                                                                                                                                           AGGCGTGTTAACCGCAGAAGAGCAGGCGCAACTGGAAGAGGCGCTGAACGTGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGG  >  NC_000913/4156990‑4157270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑