Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,339,313	A→G	R403R (CGT→CGC)	adiA ←	arginine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,339,313	0	A	G	100.0%	183.4 / NA	52	R403R (CGT→CGC)	adiA	arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/22);  total (30/22)

ATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCACGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAAC  >  NC_000913/4339170‑4339458                                                                                                                                                |                                                                                                                                                  aTCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCtt                                                                                                                                            <  2:303407/151‑1 (MQ=255)        ccTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTAc                                                                                                                                     >  2:294917/1‑151 (MQ=255)           gtgtCAGTGACAGGCCGCTGTTGCCGTCTATCCTCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGCAGGCCTGGTTGAAGCGGGAGAAGGTAATCGCCCCGCGACCTTCACGCACATg                                                                                                                                  >  2:241078/1‑151 (MQ=255)           gtgtCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACa                                                                                                                                    <  2:418207/149‑1 (MQ=255)            tgtCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATg                                                                                                                                  >  1:379354/1‑150 (MQ=255)                   gACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATaag                                                                                                                          <  2:668139/151‑1 (MQ=255)                           gCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCtgt                                                                                                                  <  1:128743/151‑1 (MQ=255)                                  ccGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAgcg                                                                                                           <  2:572785/151‑1 (MQ=255)                                  ccGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAgc                                                                                                            <  2:596972/150‑1 (MQ=255)                                           atcGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTcagc                                                                                                   >  1:13901/1‑150 (MQ=255)                                             cgccacCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGt                                                                                                <  1:7827/148‑1 (MQ=255)                                             cGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGt                                                                                                <  2:460920/151‑1 (MQ=255)                                                cacCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTtg                                                                                             <  1:307926/151‑1 (MQ=255)                                                 acCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTtgt                                                                                            <  2:429433/151‑1 (MQ=255)                                                     ccACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTgtgggt                                                                                        >  1:207624/1‑151 (MQ=255)                                                         gtcgtTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTgtgggtgg                                                                                      >  1:526337/1‑149 (MQ=255)                                                           cgtTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAgt                                                                                   <  2:359846/150‑1 (MQ=255)                                                             ttGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCG‑CCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAgtgggt                                                                               >  1:103834/1‑151 (MQ=255)                                                                  tGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCg                                                                           >  2:175903/1‑151 (MQ=255)                                                                       aGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAAc                                                                      >  1:369439/1‑151 (MQ=255)                                                                         aTGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACgg                                                                    >  1:185965/1‑151 (MQ=255)                                                                          tGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGt                                                                   >  2:558185/1‑151 (MQ=255)                                                                           ggCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTa                                                                  <  1:283023/151‑1 (MQ=255)                                                                              aTACAGCGGGGAGGTGGTGGCATTCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGCCCTTCACGTACCTGAATATAAGACGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGACAACGGt                                                                   >  2:501683/1‑147 (MQ=255)                                                                                 cAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCg                                                            >  1:450314/1‑151 (MQ=255)                                                                                       ggAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTtgtg                                                       >  1:613936/1‑150 (MQ=255)                                                                                        gAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGAt                                                     <  1:499323/151‑1 (MQ=255)                                                                                          ggtggtGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCg                                                   >  1:171961/1‑151 (MQ=255)                                                                                                ggCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGt                                             <  1:674473/151‑1 (MQ=255)                                                                                                 gcatgcagCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTGGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAgg                                              <  2:128198/149‑1 (MQ=255)                                                                                                  catgcatCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTc                                           <  2:318566/151‑1 (MQ=255)                                                                                                   atgcatCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGGTc                                           >  1:168948/1‑150 (MQ=255)                                                                                                    tgcatCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTcgc                                         >  2:98282/1‑151 (MQ=255)                                                                                                      catcatGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTcgccg                                       >  1:192817/1‑151 (MQ=255)                                                                                                       atcatGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTcgccgc                                      <  1:175908/151‑1 (MQ=255)                                                                                                         catGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCgcgc                                    >  1:617278/1‑151 (MQ=255)                                                                                                           tGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCAt                                  >  2:494196/1‑151 (MQ=255)                                                                                                                  cTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCAt                             >  1:429351/1‑149 (MQ=255)                                                                                                                    ggTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAg                           <  1:551395/149‑1 (MQ=255)                                                                                                                      ttGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGAt                       >  2:187455/1‑151 (MQ=255)                                                                                                                       tGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGAt                       <  2:662434/150‑1 (MQ=255)                                                                                                                        gAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCg                     >  1:609410/1‑151 (MQ=255)                                                                                                                         aaGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCgg                    >  1:481575/1‑151 (MQ=255)                                                                                                                         aaGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCgg                    <  1:478891/151‑1 (MQ=255)                                                                                                                         aaGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCgg                    >  1:23818/1‑151 (MQ=255)                                                                                                                           gCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCTGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGAGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCg                     >  2:521809/1‑148 (MQ=255)                                                                                                                              ggAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCAt                 >  1:284502/1‑149 (MQ=255)                                                                                                                              ggAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAg               <  2:77514/151‑1 (MQ=255)                                                                                                                                  aaGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCAt                 >  1:388115/1‑145 (MQ=255)                                                                                                                                  aaGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCg           <  2:608193/151‑1 (MQ=255)                                                                                                                                     ttAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGt        >  1:426674/1‑151 (MQ=255)                                                                                                                                           gCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAAc  >  2:411642/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                  ATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCACGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAAC  >  NC_000913/4339170‑4339458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑