Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,381,504	T→C	E272G (GAA→GGA)	frdA ←	anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,381,504	0	T	C	100.0%	144.3 / NA	42	E272G (GAA→GGA)	frdA	anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/23);  total (19/23)

CCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGC  >  NC_000913/4381357‑4381647                                                                                                                                                    |                                                                                                                                                ccGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCg                                                                                                                                              <  1:97119/151‑1 (MQ=255)  cGAGGTGACGCAAGTCGAGATAGACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCgg                                                                                                                                             >  2:427278/1‑151 (MQ=255)   gAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCggg                                                                                                                                            >  2:465152/1‑151 (MQ=255)    aGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGc                                                                                                                                           >  2:342616/1‑151 (MQ=255)      gTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGccc                                                                                                                                         <  2:344241/151‑1 (MQ=255)           gCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGcc                                                                                                                                    <  1:390254/151‑1 (MQ=255)                tCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAAt                                                                                                                               >  2:587923/1‑151 (MQ=255)                 cGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAAt                                                                                                                               <  2:615096/150‑1 (MQ=255)                       aaaCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCtt                                                                                                                            >  1:61331/1‑147 (MQ=255)                        aaCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGc                                                                                                                          >  1:315738/1‑148 (MQ=255)                         aCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGc                                                                                                                          >  1:560884/1‑147 (MQ=255)                                      gcggcgTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCAtt                                                                                                         <  2:678976/151‑1 (MQ=255)                                      gcggcgTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCAtt                                                                                                         >  2:241698/1‑151 (MQ=255)                                        ggcgTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCAtttt                                                                                                       <  2:49935/151‑1 (MQ=255)                                             ggAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTttgttg                                                                                                  <  1:396433/151‑1 (MQ=255)                                                gaTGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGAcc                                                                                               <  1:115917/151‑1 (MQ=255)                                                  tGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGAcc                                                                                               <  2:244711/149‑1 (MQ=255)                                                      gtTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAAt                                                                                          <  1:244340/150‑1 (MQ=255)                                                         gCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCg                                                                                      >  1:426656/1‑151 (MQ=255)                                                             ttACGCCATTCGTGGCATAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGATTTCCCGGGCCCAGGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGc                                                                                     <  2:32672/148‑1 (MQ=255)                                                                cGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCAc                                                                               >  1:537742/1‑151 (MQ=255)                                                                           ccAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCtt                                                                                  >  1:607193/1‑137 (MQ=255)                                                                           ccAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCtt                                                                                  <  2:607175/137‑1 (MQ=255)                                                                           ccAGAAGGCCGGAGAGCCTTTTTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCCCGCCCAGCGGATTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCa                                                                        <  2:304351/147‑1 (MQ=255)                                                                            cAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCtt                                                                   <  1:18931/151‑1 (MQ=255)                                                                               aaGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTc                                                                  <  2:672352/149‑1 (MQ=255)                                                                                     tGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGtcatc                                                           >  1:368672/1‑150 (MQ=255)                                                                                                            cAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCg                                   <  2:27564/151‑1 (MQ=255)                                                                                                              gTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTTCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGt                                 >  2:433521/1‑151 (MQ=255)                                                                                                                    tatatTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGt                            <  1:240624/150‑1 (MQ=255)                                                                                                                     atatTTGTTTTGCGGCTCGCCCAGCGGTGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTg                           <  1:100605/150‑1 (MQ=255)                                                                                                                      tatTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTTCAGATCACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGAt                         >  2:174229/1‑151 (MQ=255)                                                                                                                      tatTTGTTTTTCGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGa                          <  1:503425/150‑1 (MQ=255)                                                                                                                            tttttCGGCTCGCCCAGGGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGa                    <  2:453103/150‑1 (MQ=255)                                                                                                                            tttttCGGCCCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGa                    <  2:382932/150‑1 (MQ=255)                                                                                                                             ttttGGGCTCGCCCAGCGCAGTTCCCAGGCCAATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACt                      <  2:29933/147‑1 (MQ=255)                                                                                                                                 cGGCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAAt              <  1:190273/151‑1 (MQ=255)                                                                                                                                   gCTCGCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTc            >  1:562453/1‑151 (MQ=255)                                                                                                                                       gCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATg        >  2:159068/1‑151 (MQ=255)                                                                                                                                         ccAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTc      >  1:129847/1‑151 (MQ=255)                                                                                                                                          cAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTc      >  2:621739/1‑150 (MQ=255)                                                                                                                                             cGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGc  >  1:325240/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                CCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGC  >  NC_000913/4381357‑4381647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑