Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,391,867	A→G	A88A (GCA→GCG)	orn →	oligoribonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,391,867	0	A	G	100.0%	118.6 / NA	35	A88A (GCA→GCG)	orn	oligoribonuclease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/15);  total (20/15)

TCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTATCTCGATGTCAGCACCC  >  NC_000913/4391720‑4392012                                                                                                                                                    |                                                                                                                                                  tCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACg                                                                                                                                                >  2:264109/1‑151 (MQ=255)                       gCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTggg                                                                                                                          >  2:345628/1‑151 (MQ=255)                          gTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGc                                                                                                                       >  1:391571/1‑151 (MQ=255)                                      gatgaACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAAt                                                                                                           <  1:327384/151‑1 (MQ=255)                                      gatgaACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAAt                                                                                                           >  1:8022/1‑151 (MQ=255)                                       atgaACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATc                                                                                                          >  2:498602/1‑151 (MQ=255)                                       atgaACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATc                                                                                                          <  1:632233/151‑1 (MQ=255)                                        tgaACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAATATCg                                                                                                         >  2:658451/1‑151 (MQ=255)                                               cTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGAttt                                                                                                  >  1:405831/1‑151 (MQ=255)                                                       gatggatgACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTa                                                                                            <  2:112732/149‑1 (MQ=255)                                                          ggatgACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGc                                                                                       <  1:97071/151‑1 (MQ=255)                                                              gACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATc                                                                                    <  2:523411/150‑1 (MQ=255)                                                              gACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCg                                                                                   >  2:8961/1‑151 (MQ=255)                                                                     aCGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAgg                                                                             >  1:15565/1‑150 (MQ=255)                                                                     aCGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAgg                                                                             >  2:354797/1‑150 (MQ=255)                                                                     aCGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAgg                                                                             >  1:111368/1‑150 (MQ=255)                                                                      cGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGAc                                                                           >  2:150175/1‑151 (MQ=255)                                                                         gcgcACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACcgt                                                                        <  1:240837/151‑1 (MQ=255)                                                                                    ccGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGtt                                                             <  1:310935/151‑1 (MQ=255)                                                                                           cGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATAc                                                      >  1:226080/1‑151 (MQ=255)                                                                                               cTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGc                                                  >  1:188440/1‑151 (MQ=255)                                                                                                    agagCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGa                                              <  1:575250/150‑1 (MQ=255)                                                                                                             gAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGcc                                    >  1:301698/1‑151 (MQ=255)                                                                                                               aaGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTa                                  <  2:79189/151‑1 (MQ=255)                                                                                                                       aCGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACt                          <  1:80277/151‑1 (MQ=255)                                                                                                                        cGATGGGCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACt                          >  1:58893/1‑150 (MQ=255)                                                                                                                            gggCGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGt                     <  1:671166/151‑1 (MQ=255)                                                                                                                               cGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTAt                  <  2:188435/151‑1 (MQ=255)                                                                                                                               cGATCGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTAt                  >  1:593824/1‑151 (MQ=255)                                                                                                                                   cGGGAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTATCTCg              <  2:110291/151‑1 (MQ=255)                                                                                                                                    gggAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTATCTCg              >  1:652620/1‑150 (MQ=255)                                                                                                                                    gggAAGCTGAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTATCTCGa             <  1:264114/151‑1 (MQ=255)                                                                                                                                      gAAGTTGAACTGGCGACGCTTGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTAtc                 <  1:220765/145‑1 (MQ=255)                                                                                                                                            gAACTGGCGACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTATCTCGATGTCAGc      >  1:5462/1‑150 (MQ=255)                                                                                                                                               cTGGCGACGCTCGAATTTTTAAAACAGCGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTATCTCGATGTCAGCAccc  >  2:613562/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTATCTCGATGTCAGCACCC  >  NC_000913/4391720‑4392012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑