Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,407,982	T→C	R443R (CGT→CGC)	rnr →	exoribonuclease R, RNase R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,407,982	0	T	C	100.0%	165.0 / NA	44	R443R (CGT→CGC)	rnr	exoribonuclease R, RNase R
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/23);  total (21/23)

GCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCG  >  NC_000913/4407841‑4408127                                                                                                                                              |                                                                                                                                                  gcgcGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGaa                                                                                                                                             <  1:156701/148‑1 (MQ=255) gcgcGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGt                                                                                                                                          >  2:398093/1‑151 (MQ=255)  cgcgAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGt                                                                                                                                          >  2:679299/1‑150 (MQ=255)   gcgAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTAt                                                                                                                                        <  1:634923/151‑1 (MQ=255)   gcgAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTAt                                                                                                                                        >  2:568211/1‑151 (MQ=255)   gcgAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGAGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTAt                                                                                                                                        >  2:596434/1‑151 (MQ=255)         aGTACGCTCCGCTGGTTAAGCATCTCGGAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAAc                                                                                                                                   >  2:665518/1‑150 (MQ=255)                   gCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCg                                                                                                                         >  2:79487/1‑150 (MQ=255)                         tAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAacgac                                                                                                                  >  1:40240/1‑151 (MQ=255)                          aaGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGGCGCATTGAACGTATCGAACAGACCCAGCGTAacgac                                                                                                                  <  2:374990/150‑1 (MQ=255)                          aaGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAacgac                                                                                                                  <  1:99016/150‑1 (MQ=255)                          aaGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAacgac                                                                                                                  <  2:679649/150‑1 (MQ=255)                            gCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGAcgcg                                                                                                               <  2:59631/151‑1 (MQ=255)                             cATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACgcgc                                                                                                              <  2:439447/151‑1 (MQ=255)                                  cGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGca                                                                                                             <  2:572397/147‑1 (MQ=255)                                  cGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACaa                                                                                                          >  2:645558/1‑150 (MQ=255)                                      gagTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAaattaa                                                                                                     <  1:113611/151‑1 (MQ=255)                                      gagTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAaatta                                                                                                      >  1:672912/1‑150 (MQ=255)                                            cATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAag                                                                                               <  1:187284/151‑1 (MQ=255)                                                  ctctATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGc                                                                                          >  1:274609/1‑150 (MQ=255)                                                      ataAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCAt                                                                                        <  1:390943/148‑1 (MQ=255)                                                                ggATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGa                                                                             <  1:120232/149‑1 (MQ=255)                                                                       gCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTcgg                                                                    <  2:213821/151‑1 (MQ=255)                                                                       gCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTcgg                                                                    <  1:671291/151‑1 (MQ=255)                                                                        cccGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTcggc                                                                   <  1:95125/151‑1 (MQ=255)                                                                         ccGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTcggcg                                                                  <  1:367598/151‑1 (MQ=255)                                                                           gTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCggcggc                                                                >  1:223825/1‑151 (MQ=255)                                                                                       gTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTg                                                     >  1:381994/1‑150 (MQ=255)                                                                                             tctcATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGc                                              >  2:134392/1‑151 (MQ=255)                                                                                               tcATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCg                                             >  2:27577/1‑150 (MQ=255)                                                                                                      agagCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAAcc                                     <  1:289628/151‑1 (MQ=255)                                                                                                         gCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGc                                  >  2:266850/1‑151 (MQ=255)                                                                                                           gaagaaGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCAc                                <  1:61872/151‑1 (MQ=255)                                                                                                                    aaGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCg                         >  1:339759/1‑149 (MQ=255)                                                                                                                        tCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTc                    >  2:311188/1‑150 (MQ=255)                                                                                                                         cATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCa                   <  2:518447/150‑1 (MQ=255)                                                                                                                             ttCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGAc               <  1:481246/150‑1 (MQ=255)                                                                                                                              tCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACa              >  1:376579/1‑150 (MQ=255)                                                                                                                                 aCGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGcc          <  2:241498/151‑1 (MQ=255)                                                                                                                                    cTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCg         >  2:166340/1‑149 (MQ=255)                                                                                                                                      gAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAg       <  2:487205/149‑1 (MQ=255)                                                                                                                                      gAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAg       <  2:469242/149‑1 (MQ=255)                                                                                                                                         cgccgcATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCg  <  2:11180/151‑1 (MQ=255)                                                                                                                                          gCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCg  >  1:376293/1‑150 (MQ=255)                                                                                                                                          gCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCg  >  2:575704/1‑150 (MQ=255)                                                                                                                                              |                                                                                                                                                  GCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCG  >  NC_000913/4407841‑4408127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑