Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,491,790	A→G	L246L (TTA→CTA)	idnT ←	L‑idonate and D‑gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,491,790	0	A	G	100.0%	190.8 / NA	54	L246L (TTA→CTA)	idnT	L‑idonate and D‑gluconate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/29);  total (25/29)

AATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATAAGCCTTCCGGTGGTGCTTTCTCAAAGCGAGTTA  >  NC_000913/4491643‑4491936                                                                                                                                                    |                                                                                                                                                   aaTCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGt                                                                                                                                                 <  1:125108/151‑1 (MQ=255)       cAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATACCAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTc                                                                                                                                           <  1:296800/151‑1 (MQ=255)            atatCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTcaca                                                                                                                                        <  2:548318/149‑1 (MQ=255)                ccATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAAcggc                                                                                                                                  <  2:365387/151‑1 (MQ=255)                ccATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAAc                                                                                                                                     <  2:517674/148‑1 (MQ=255)                       ttGCTCGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGAt                                                                                                                            <  1:278801/150‑1 (MQ=255)                              aTGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATc                                                                                                                     >  2:520804/1‑150 (MQ=255)                                  tGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGAt                                                                                                                >  2:219723/1‑151 (MQ=255)                                  tGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGAt                                                                                                                >  1:171507/1‑151 (MQ=255)                                       ccATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGAcc                                                                                                            <  2:513694/150‑1 (MQ=255)                                        cATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCgg                                                                                                          <  1:258876/151‑1 (MQ=255)                                              gTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCAc                                                                                                    <  1:55790/151‑1 (MQ=255)                                              gTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCAc                                                                                                    <  2:437292/151‑1 (MQ=255)                                                  gCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCa                                                                                                <  2:127576/151‑1 (MQ=255)                                                  gCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCa                                                                                                <  1:174214/151‑1 (MQ=255)                                                          tgAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAAt                                                                                        >  1:42050/1‑151 (MQ=255)                                                          tgAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAAt                                                                                        >  1:536675/1‑151 (MQ=255)                                                           gAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAAt                                                                                        >  2:147837/1‑150 (MQ=255)                                                            aaaaTCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGCGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATAc                                                                                      <  2:274840/151‑1 (MQ=255)                                                                 cGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTc                                                                                 >  1:654058/1‑151 (MQ=255)                                                                   cAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTcc                                                                                >  2:420753/1‑150 (MQ=255)                                                                   cAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTcc                                                                                >  1:59648/1‑150 (MQ=255)                                                                   cAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCa                                                                               <  2:638867/151‑1 (MQ=255)                                                                    aaTAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTcc                                                                                >  2:403505/1‑149 (MQ=255)                                                                         cAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAagga                                                                         <  1:631967/151‑1 (MQ=255)                                                                         cAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAagg                                                                          >  1:444810/1‑150 (MQ=255)                                                                          aaTGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAagg                                                                          >  1:303145/1‑149 (MQ=255)                                                                                    aaCAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATctcct                                                              <  1:491019/151‑1 (MQ=255)                                                                                      cAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCctct                                                            >  1:359050/1‑151 (MQ=255)                                                                                      cAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCctct                                                            >  2:182192/1‑151 (MQ=255)                                                                                       aGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCtt                                                           >  2:241457/1‑151 (MQ=255)                                                                                        gcgcAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTc                                                          >  2:164907/1‑151 (MQ=255)                                                                                        gcgcAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCAGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTc                                                          <  1:357734/151‑1 (MQ=255)                                                                                          gcAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCTTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGc                                                        <  2:677035/151‑1 (MQ=255)                                                                                             aCGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTaa                                                     <  1:430225/151‑1 (MQ=255)                                                                                                      ttACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATg                                              >  1:373362/1‑149 (MQ=255)                                                                                                      ttACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATg                                              >  1:383755/1‑149 (MQ=255)                                                                                                        aCCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAgg                                           >  1:7663/1‑150 (MQ=255)                                                                                                            aCAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGTCATCTCCTCTTCGCTAAACAGATGAGGATTa                                       <  1:442638/150‑1 (MQ=255)                                                                                                              aaaTTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAAt                                    >  2:104255/1‑151 (MQ=255)                                                                                                                aTTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATaa                                  <  2:604510/151‑1 (MQ=255)                                                                                                                aTTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATaa                                  <  2:665949/151‑1 (MQ=255)                                                                                                                aTTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATa                                   >  2:560844/1‑150 (MQ=255)                                                                                                                 ttCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATa                                   >  1:493079/1‑149 (MQ=255)                                                                                                                      agaagaGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATAAGCCTTc                            <  1:560861/151‑1 (MQ=255)                                                                                                                       gaagaGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATAAGCCTTcc                           <  1:591552/151‑1 (MQ=255)                                                                                                                       gaagaGGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATAAGCCTTcc                           <  1:181139/151‑1 (MQ=255)                                                                                                                           agGCGCACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATAAGCCTTCCggtg                       <  2:618168/151‑1 (MQ=255)                                                                                                                              cgcACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATAAGCCTTCCGgtggtg                    >  2:200562/1‑151 (MQ=255)                                                                                                                                cACGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATAAGCCTTCCGGTGGTGCt                  <  1:131219/151‑1 (MQ=255)                                                                                                                                 aCGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATAAGCCTTCCGGTGGTGCtt                 <  1:417295/151‑1 (MQ=255)                                                                                                                                  cGGTGTTAGTTTTCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATAAGCCTTCCGGTGGTGCttt                >  1:571844/1‑151 (MQ=255)                                                                                                                                            tttCGGTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATAAGCCTTCCGGTGGTGCTTTCTCAAAGCg       >  1:671820/1‑150 (MQ=255)                                                                                                                                                ggTAGCGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATAAGCCTTCCGGTGGTGCTTTCTCAAAGCGAGTTa  <  1:520818/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   AATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAAATCGCAATAACAATGGCAATAAACAGCGCAACGGCAGGGTTACCGACAAATTCAAAGAAGAGGCGCACGGTGTTAGTTTTCGGTAACGTAATTTCACAAACGGCGGCGATAGCCATCAGGATGACCGGGATCACGGCAGCGAAAATACTGTTCCAGAAGGAGGGCATCTCCTCTTCGCTAAACAGATGAGGATTAAATAAGCCTTCCGGTGGTGCTTTCTCAAAGCGAGTTA  >  NC_000913/4491643‑4491936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑