Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,641,366	T→C	L142P (CTC→CCC)	yjtD →	putative methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,641,366	0	T	C	100.0%	99.4 / NA	28	L142P (CTC→CCC)	yjtD	putative methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/14);  total (14/14)

CTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAG  >  NC_000913/4641221‑4641509                                                                                                                                                  |                                                                                                                                                cTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATc                                                                                                                                            >  1:356393/1‑151 (MQ=255)        cGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGc                                                                                                                                      >  1:591238/1‑150 (MQ=255)                   gAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATgg                                                                                                                          >  1:309959/1‑151 (MQ=255)                     aaaaTCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGt                                                                                                                         >  1:545435/1‑150 (MQ=255)                       aaTCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGAGACgg                                                                                                                          >  2:71376/1‑147 (MQ=255)                            tCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTg                                                                                                                   <  1:283178/149‑1 (MQ=255)                                 gATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCaa                                                                                                            <  2:75338/151‑1 (MQ=255)                                     aGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAAtt                                                                                                          >  1:677397/1‑149 (MQ=255)                                     aGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAg                                                                                                        <  2:107643/151‑1 (MQ=255)                                       ccATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCa                                                                                                      <  2:518273/151‑1 (MQ=255)                                        cATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCaa                                                                                                     <  1:434784/151‑1 (MQ=255)                                           gCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACa                                                                                                   <  2:26019/150‑1 (MQ=255)                                            ccGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACAtt                                                                                                 <  2:501473/151‑1 (MQ=255)                                                                        cGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCa                                                                     <  1:485473/151‑1 (MQ=255)                                                                                    cGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCaa                                                         <  2:194775/151‑1 (MQ=255)                                                                                                      tGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGAGCAAGATCAACTGCAAGCttt                                        >  1:40759/1‑150 (MQ=255)                                                                                                      tGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCttt                                        >  1:490843/1‑150 (MQ=255)                                                                                                       gACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTa                                       <  2:583351/150‑1 (MQ=255)                                                                                                       gACGTTCTTAATGGTGTTACGATTGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGTTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCACGCTTTa                                       <  2:539248/150‑1 (MQ=255)                                                                                                              ttACTGGTGTGCCGATGGCGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGCGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGaa                                 >  2:299059/1‑149 (MQ=255)                                                                                                                   ggTGTGCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGcc                           <  1:548898/150‑1 (MQ=255)                                                                                                                      gtgCCGATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATg                        <  2:48955/150‑1 (MQ=255)                                                                                                                           gATGGTGGCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACAtt                   >  2:648426/1‑150 (MQ=255)                                                                                                                              ggtggCGGATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTg               <  1:439935/151‑1 (MQ=255)                                                                                                                                    ggATTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTgacgac          >  1:597807/1‑150 (MQ=255)                                                                                                                                      aTTATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGActct       >  2:487103/1‑151 (MQ=255)                                                                                                                                       ttATCCTTCGCCCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCtg      >  1:604425/1‑151 (MQ=255)                                                                                                                                              tCGCCCAATCTGGGGCAGGCGGTGTTGGTCTATTGCTATCAATTATCAACATTAATACTACAACCGGCGAAAAGTGATGCAACGGCAAACCAACATCAACTGCAACCTTTACGCCAACCAGCCACGACATTTCTGGCGACTCtgggcg  >  2:119786/1‑145 (MQ=255)                                                                                                                                                  |                                                                                                                                                CTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAG  >  NC_000913/4641221‑4641509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑