Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	667,534	T→C	E228E (GAA→GAG)	mrdA ←	penicillin‑binding protein 2, transpeptidase involved in peptidoglycan synthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	667,534	0	T	C	100.0%	158.3 / NA	42	E228E (GAA→GAG)	mrdA	penicillin‑binding protein 2, transpeptidase involved in peptidoglycan synthesis
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/21);  total (21/21)

TCGGTGACTACCACAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTT  >  NC_000913/667388‑667677                                                                                                                                                   |                                                                                                                                                tCGGTGACTACCACAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTc                                                                                                                                               <  1:377848/149‑1 (MQ=255) tCGGTGACTACCACAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCa                                                                                                                                              >  1:484599/1‑150 (MQ=255)           ccACAGCTGCGCGGCTCCCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTc                                                                                                                                      <  2:237989/148‑1 (MQ=255)                   gcgcGGCTCCCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTc                                                                                                                            <  2:586752/150‑1 (MQ=255)                   gcgcGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCt                                                                                                                           >  2:332155/1‑151 (MQ=255)                       ggCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGAc                                                                                                                        <  1:445923/150‑1 (MQ=255)                              cGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTgcagc                                                                                                                >  1:438373/1‑151 (MQ=255)                                        gTTTCAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCAt                                                                                                      >  1:577435/1‑151 (MQ=255)                                            cAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGt                                                                                                   >  1:27316/1‑150 (MQ=255)                                            cAATATATTGCTGGAGTTTGAGAGCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTCTTAACTGTCGAATAACACGCCCACGGTTGTTAACCTCAACCTTTTCATAACCGGTCTGACCGTGCAGCACCTCTTCATCGt                                                                                                   <  2:234091/150‑1 (MQ=255)                                                   ttGCTGGAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTc                                                                                            >  1:436629/1‑150 (MQ=255)                                                    tGCTGGAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCa                                                                                           <  1:517846/150‑1 (MQ=255)                                                         gAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGccc                                                                                     >  2:446766/1‑151 (MQ=255)                                                          aGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCa                                                                                    <  2:232751/151‑1 (MQ=255)                                                                    ccAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGa                                                                           <  1:1892/150‑1 (MQ=255)                                                                          tCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATg                                                                    >  2:422325/1‑151 (MQ=255)                                                                              gTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACGCGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGtt                                                                <  2:278991/151‑1 (MQ=255)                                                                                aaaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGc                                                              >  1:201986/1‑151 (MQ=255)                                                                                aaaTATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGc                                                              >  1:576239/1‑151 (MQ=255)                                                                                      cGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCAt                                                         <  2:273713/150‑1 (MQ=255)                                                                                       gtgtCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAg                                                       <  2:264837/151‑1 (MQ=255)                                                                                       gtgtCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAg                                                       <  2:27316/151‑1 (MQ=255)                                                                                            cGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGc                                                  <  1:605284/151‑1 (MQ=255)                                                                                                ttGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGt                                              >  2:4301/1‑151 (MQ=255)                                                                                                  gCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGttt                                            <  2:196604/151‑1 (MQ=255)                                                                                                      tggtACTTCTTTTAACTGGCGAATAACACGCGCACGGTTGTTAACCTCAACCTCTTCATATCCGGTCTGTCCGTGCAGCTCATCCTCATAGTATCGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCTCATAGTTGGCCAgg                                              <  2:597034/145‑2 (MQ=255)                                                                                                       ggtACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGGCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGt                                       <  2:438646/151‑1 (MQ=255)                                                                                                       ggtACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGt                                       >  1:303066/1‑151 (MQ=255)                                                                                                       ggtACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGt                                       >  2:468314/1‑151 (MQ=255)                                                                                                           cttcttTTAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCatt                                   >  1:561524/1‑151 (MQ=255)                                                                                                               ttttAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTTGCCAGTTTGCCGTCATTATTc                               >  1:34240/1‑151 (MQ=255)                                                                                                                tttAACTGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCattatt                                <  2:452523/149‑1 (MQ=255)                                                                                                                      tGGCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGtt                        >  1:446254/1‑151 (MQ=255)                                                                                                                       ggCGAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTc                       >  1:532186/1‑151 (MQ=255)                                                                                                                          gAATAACACGCCCGCGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGGGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTTGCCAGTTTGCCGTCATTATTCAGGCGTTCGAc                    <  2:471893/151‑1 (MQ=255)                                                                                                                          gAATAACACGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGAc                    >  1:434642/1‑151 (MQ=255)                                                                                                                                 acGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCttt              >  1:363005/1‑150 (MQ=255)                                                                                                                                 acGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTa             <  2:29156/151‑1 (MQ=255)                                                                                                                                  cGCCCACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTAt            >  2:82914/1‑151 (MQ=255)                                                                                                                                   gCCAACGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCCGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTa             >  2:515204/1‑149 (MQ=255)                                                                                                                                        cGGTTGTTAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTg       <  2:350431/150‑1 (MQ=255)                                                                                                                                             gttAACCTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGAtttt  <  1:528854/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                TCGGTGACTACCACAGCTGCGCGGCTACCCGCCAGCAGCGTTTCAATATATTGCTGGAGTTTGAGATCCAGCGTCAGGTAAATATCGTGTCCGGCTTGCGGTGGTACTTCTTTTAACTGGCGAATAACACGCCCACGGTTGTTAACTTCAACCTCTTCATAACCGGTCTGACCGTGCAGCACATCTTCATAGTAACGCTCAATGCCCAGCTTACCGATATCATGCGTTGCCGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTT  >  NC_000913/667388‑667677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑