Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	736,382	T→C	intergenic (+163/‑63)	ybfC → / → ybfQ	putative secreted protein/pseudogene, H repeat‑associated protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	736,382	0	T	C	100.0%	166.4 / NA	44	intergenic (+163/‑63)	ybfC/ybfQ	putative secreted protein/pseudogene, H repeat‑associated protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/23);  total (21/23)

CAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGTCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTCAGAT  >  NC_000913/736238‑736528                                                                                                                                                 |                                                                                                                                                   cAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTaa                                                                                                                                                >  1:72962/1‑149 (MQ=255)     ttAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGt                                                                                                                                          >  1:472111/1‑151 (MQ=255)       aaTCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGttt                                                                                                                                        <  1:618016/151‑1 (MQ=255)         tCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATATTACTTATTGCCTAAAGCTGttttt                                                                                                                                      >  2:407133/1‑151 (MQ=255)           aTTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTc                                                                                                                                     >  2:440849/1‑150 (MQ=255)               tgAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTACCTAAAGCTGttttt                                                                                                                                      >  2:635878/1‑145 (MQ=255)                  aaaaTTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTta                                                                                                                             <  1:186192/151‑1 (MQ=255)                    aaTTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTtata                                                                                                                           <  2:544857/151‑1 (MQ=255)                        atataACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATg                                                                                                                       >  1:292629/1‑151 (MQ=255)                             aCTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATc                                                                                                                    >  2:256390/1‑149 (MQ=255)                                cATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAAt                                                                                                                <  1:36555/150‑1 (MQ=255)                                cATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATa                                                                                                               <  1:531011/151‑1 (MQ=255)                                      aTAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCtt                                                                                                         <  2:72959/151‑1 (MQ=255)                                        aGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTAc                                                                                                       >  1:416743/1‑151 (MQ=255)                                         ggTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACa                                                                                                      <  2:356304/151‑1 (MQ=255)                                         ggTGTTTATTTTACAGTCCAATTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCt                                                                                                          <  2:472099/147‑1 (MQ=14)                                            gtTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACata                                                                                                    <  1:260684/150‑1 (MQ=255)                                                  tttGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAgg                                                                                             >  1:234102/1‑151 (MQ=255)                                                     gCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTg                                                                                           <  2:266447/150‑1 (MQ=255)                                                            aTTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATAATCAAATACTCCTTACATAAATAAGGGGAACAAAt                                                                                    >  2:245836/1‑150 (MQ=255)                                                              ttGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATgg                                                                                  <  1:111665/150‑1 (MQ=255)                                                                gCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGaa                                                                                <  2:381375/150‑1 (MQ=255)                                                                 cAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAgagatcggaatag                                                                                  >  2:196610/1‑135 (MQ=255)                                                                        aGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTaaaaaa                                                                       <  2:240428/151‑1 (MQ=255)                                                                            cACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTg                                                                    <  1:308973/150‑1 (MQ=255)                                                                                tAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATgg                                                                >  1:95092/1‑150 (MQ=255)                                                                                 aaCGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGa                                                               >  2:558888/1‑150 (MQ=255)                                                                                  aCGCTACTTTTTTGTACTAAATAATTCGAATCTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACGCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGaa                                                              <  2:84517/150‑1 (MQ=255)                                                                                      tACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACatat                                                         >  1:126139/1‑151 (MQ=255)                                                                                              tGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTAtt                                                  <  1:488605/150‑1 (MQ=255)                                                                                                    aaaTAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCg                                           >  2:310107/1‑151 (MQ=255)                                                                                                        aatTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTa                                       <  2:555854/151‑1 (MQ=255)                                                                                                          ttCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTAc                                      >  1:186662/1‑150 (MQ=255)                                                                                                                 tttAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGc                              >  2:366636/1‑151 (MQ=255)                                                                                                                 tttAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTAGTACACCCAATgacagaca                                 >  2:386337/1‑148 (MQ=255)                                                                                                                            caaTTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTAGACCCGATTACAGACAAGCCTGGAAAgt                     >  2:48847/1‑149 (MQ=255)                                                                                                                            caaTTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAgtgg                   >  2:451266/1‑151 (MQ=255)                                                                                                                                 gagaTACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCa               <  1:584566/150‑1 (MQ=255)                                                                                                                                 gagaTACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCAt              <  1:366646/151‑1 (MQ=255)                                                                                                                                   gaTACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATaa            <  1:229754/151‑1 (MQ=255)                                                                                                                                         actTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTg        <  1:30091/149‑1 (MQ=255)                                                                                                                                         actTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGAGCATAAATTGt       >  1:543657/1‑150 (MQ=255)                                                                                                                                            tATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTCAg    <  2:242931/150‑1 (MQ=255)                                                                                                                                             aTTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTCAGat  <  1:607757/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGTCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTCAGAT  >  NC_000913/736238‑736528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑