Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	758,260	A→G	D191G (GAC→GGC)	sdhB →	succinate dehydrogenase, FeS subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	758,260	0	A	G	100.0%	152.9 / NA	42	D191G (GAC→GGC)	sdhB	succinate dehydrogenase, FeS subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/19);  total (23/19)

CGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCG  >  NC_000913/758117‑758402                                                                                                                                                |                                                                                                                                               cGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAgccgc                                                                                                                                         <  2:293040/151‑1 (MQ=255)    cGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTc                                                                                                                                      >  1:661214/1‑151 (MQ=255)           tATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGt                                                                                                                                <  2:591542/150‑1 (MQ=255)                  gTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTATCGGCATATCGTTTCCTGATTTATAGCCGTGATACCGAGCCTGGCAGCCGCCTCGACGGTTTGAgcg                                                                                                                         >  1:516696/1‑148 (MQ=255)                    aTTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGAt                                                                                                                       >  1:219844/1‑150 (MQ=255)                               aTGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGGTTGAGTGATGCATTCAGCGt                                                                                                            >  1:49098/1‑150 (MQ=255)                                    gttCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCCCGACGGTTTGAGTGATGCATTCAGCGGATTcc                                                                                                       >  1:445608/1‑150 (MQ=255)                                      tCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGc                                                                                                     >  2:539563/1‑150 (MQ=255)                                          ccTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTc                                                                                                 <  1:484503/150‑1 (MQ=255)                                           ctctTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTcac                                                                                               >  1:68287/1‑151 (MQ=255)                                              ttGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAg                                                                                             >  2:533200/1‑150 (MQ=255)                                               tGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTTGCGGCATATCGTTTCCTGATTGATAACCGTGGTACCGAGACTGGCAGCCGCCTCGACGGGTTGGGTGATGCCTTCAACGTATTCCGCTGGca                                                                                                >  2:643346/1‑146 (MQ=255)                                               tGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGc                                                                                            <  1:388081/150‑1 (MQ=255)                                                 tCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGcat                                                                                          <  1:52727/150‑1 (MQ=255)                                                    gTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGGTTGAGGGATGCATTCAGCGTATTCCGCTGTCACAGcatcat                                                                                       >  2:175894/1‑150 (MQ=255)                                                         tCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACt                                                                                  <  2:412247/150‑1 (MQ=255)                                                         tCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACt                                                                                  <  2:385376/150‑1 (MQ=255)                                                           tggtggAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAACCGTGATACCGAGACTGGCAGCCGCCTCGACGGGTTGAGTGATGCATTCCGCGTATTCCGCTGTCACAGCATCATGaa                                                                                    >  1:285642/1‑146 (MQ=255)                                                            ggtggAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCg                                                                               <  2:144033/150‑1 (MQ=255)                                                                gAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGGTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTc                                                                             >  2:441269/1‑148 (MQ=255)                                                                        aTAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTcc                                                                  <  1:10800/151‑1 (MQ=255)                                                                           aGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCCGATTGCTAGCCGTGATACCGAAACTGGCAGCCGCCTCGACGGGTTGAGTGATGCATTCAGCGTATTTCGCTGTCACAGCATCATTAACTGCGTCAGTGTATGTCCg                                                                 >  2:313959/1‑149 (MQ=255)                                                                              ttATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAgg                                                             <  2:649439/150‑1 (MQ=255)                                                                               tATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAggg                                                            <  1:648140/150‑1 (MQ=255)                                                                                aTCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAgggg                                                           <  1:486315/150‑1 (MQ=255)                                                                                       cGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAAccc                                                   >  1:216647/1‑151 (MQ=255)                                                                                        ggCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCg                                                  >  2:604385/1‑151 (MQ=255)                                                                                          cAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGAc                                                >  1:158832/1‑151 (MQ=255)                                                                                                tGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGAcgcgc                                            <  1:248504/149‑1 (MQ=255)                                                                                                  ttAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGcc                                         >  1:537156/1‑150 (MQ=255)                                                                                                       ggCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGc                                   >  1:454999/1‑151 (MQ=255)                                                                                                         cATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGcc                                  >  2:143420/1‑150 (MQ=255)                                                                                                          atatCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCat                                <  1:473241/151‑1 (MQ=255)                                                                                                            atCGTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCatat                              >  1:52142/1‑151 (MQ=255)                                                                                                               gTTTCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCaa                           <  1:276953/151‑1 (MQ=255)                                                                                                                  tCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGt                         <  2:560631/150‑1 (MQ=255)                                                                                                                  tCCTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTc                        >  1:649316/1‑151 (MQ=255)                                                                                                                   ccTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCg                       >  1:70401/1‑151 (MQ=255)                                                                                                                   ccTGATTGATAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCg                       >  1:425647/1‑151 (MQ=255)                                                                                                                         tgatAGCCGTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGAtgtt                  <  2:636152/150‑1 (MQ=255)                                                                                                                                 gTGATACCGAGACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACg         <  2:558801/151‑1 (MQ=255)                                                                                                                                        cGATACTGGCAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCATCGTAATGCg  <  1:643508/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                               CGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGCATCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCG  >  NC_000913/758117‑758402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑