Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	44,792	A→G	N205D (AAT→GAT)	fixC →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	44,792	0	A	G	100.0%	165.0 / NA	46	N205D (AAT→GAT)	fixC	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/23);  total (23/23)

TGCTGATGGGGTGAACTCCATCCTTGCCGAAAAATTGGGGATGGCAAAACGCGTCAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAA  >  NC_000913/44653‑44939                                                                                                                                            |                                                                                                                                                     tGCTGATGGGGTGAACTCCATCCTTGCCGAAAAATTGGGGATGGCAAAACGCGTCAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGggc                                                                                                                                            <  1:17076/150‑1 (MQ=255) tGCTGATGGGGTGAACTCCATCCTTGCCGAAAAATTGGGGATGGCAAAACGCGTCAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGggc                                                                                                                                            <  1:23292/150‑1 (MQ=255)             gAACTCCATCCTTGCCGAAAAATTGGGGATGGCAAAACGCGTCAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGttt                                                                                                                               <  2:156042/151‑1 (MQ=255)             gAACTCCATCCTTGCCGAAAAATTGGGGATGGCAAAACGCGTCAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGtt                                                                                                                                <  1:282064/150‑1 (MQ=255)              aaCTCCATCCTTGCCGAAAAATTGGGGATGGCAAAACGCGTCAAACCGACGGATGTGGCGGTTTGGGGGAAGGAAAAGAACGCGTTACCCAAATCGGTTTTTTAAAACCGTTTTTTGTTTCAGGGGGGTCAGGGGGGGGCtttgctcttt                                                                                                                               >  2:280752/1‑142 (MQ=255)                   cATCCTTGCCGAAAAATTGGGGATGGCAAAACGCGTCAAACCGACGGATGTGGCGGTTTGCGGGGAGGAAATGAACGAGGTAACCAAGAAGGGTTTTGAAAAACGGTTTTTGTTGCAGGGGGGTCAGGGGGGGGGGTGCCTGTTTTCgg                                                                                                                           >  2:577311/1‑149 (MQ=255)                              aaaaaTTGGGGATGGCAAAACGCGTCAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGa                                                                                                              <  2:214981/151‑1 (MQ=255)                                      gggATGGCAAAACGCGTCAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGa                                                                                                      <  2:43781/151‑1 (MQ=255)                                           ggCAAAACGCGTCAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATcacc                                                                                                                    <  2:345788/132‑1 (MQ=255)                                           ggCAAAACGCGTCAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGggc                                                                                                <  2:485898/151‑1 (MQ=255)                                           ggCAAAACGCGTCAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGggc                                                                                                <  1:27495/151‑1 (MQ=255)                                                   gcgTCAAACCGACGGATGTGGCGGTTTGCGGGGAGGGAATGGATGAGGTAACGAAATCGGTTATTTAAAAACGGTTTTAGTTGCAGGGGGGTCAGGGGGGGGGTTGCCCGTTTGGGGGGGTACCCCCCCATGGGCCGG‑TGgggggggg                                                                                           >  1:351033/1‑148 (MQ=255)                                                   gcgTCAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCtt                                                                                        <  2:275722/151‑1 (MQ=255)                                                     gTCAAACCGACGGATGTGGCGGTTTGCGTGGAGGAACTGATTGGGGTAACGAAGGAGGGGTTTTAAAAACGCTTTTAATTGCCAGGGGGTCAGGGGGGGGGTTTGCTTTTTTCGGGGTCACCCCCCCACGGGCTGACTGGGGGGGGGCtcc                                                                                       >  2:434323/1‑149 (MQ=255)                                                     gTCAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTcc                                                                                      <  2:169542/151‑1 (MQ=255)                                                      tCAAACCGACGGATGTGGCGGATTGGGGGGAGGAACCGATCGTGTTACCGAAGGAGGGGTTTGGAAACCGTTTTTTGTTGTAGGGGGAGTAGGGGGGGGGGTGCCTGGTTTCGGGGTCACCCCCCCATGGCCTGG‑TGGGGGGGGGCCTcc                                                                                      >  2:424110/1‑150 (MQ=255)                                                       cAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCtt                                                                                    <  2:312216/151‑1 (MQ=255)                                                        aaaCCGACGGATGTGGCGGTTGGCGGGGAGGAACTGATCGAGTTAACCAAGTCGGGTGTTGGAGACCGGTTTTAGGTGTAGGGGGGGCAGGGGGGGGGTTGCCTGTTTGCGGGATTACCCCCCCATGGGCTGA‑TGgggggcgggcttcttt                                                                                   >  2:142347/1‑143 (MQ=255)                                                                        cGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGggcggcgg                                                                                           <  1:492756/127‑1 (MQ=255)                                                                        cGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGggcggcgg                                                                                           >  2:492743/1‑127 (MQ=255)                                                                        cGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAacac                                                                   <  1:333835/151‑1 (MQ=255)                                                                              gCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTc                                                             >  2:143175/1‑151 (MQ=255)                                                                                gTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCg                                                            <  2:270446/150‑1 (MQ=255)                                                                                 tGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCt                                                          >  1:181618/1‑151 (MQ=255)                                                                                    aGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTggg                                                       <  1:384432/151‑1 (MQ=255)                                                                                            aTCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCtggttt                                               <  1:541699/151‑1 (MQ=255)                                                                                              cGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCtggtttgt                                             >  2:223725/1‑151 (MQ=255)                                                                                               gAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCtggtttgtg                                            <  2:353140/151‑1 (MQ=255)                                                                                               gAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCtggtttgtg                                            >  2:69340/1‑151 (MQ=255)                                                                                                 gTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCtggtttgtggt                                          >  1:196021/1‑151 (MQ=255)                                                                                                   tACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCtggtttgtggttt                                        >  2:495092/1‑151 (MQ=255)                                                                                                     ccGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGc                                      >  2:201784/1‑151 (MQ=255)                                                                                                           tCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCatcatc                                >  2:101807/1‑151 (MQ=255)                                                                                                             ggTTATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTg                              <  1:396479/151‑1 (MQ=255)                                                                                                               ttATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGc                             >  1:59882/1‑150 (MQ=255)                                                                                                               ttATTGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGc                             >  1:246982/1‑150 (MQ=255)                                                                                                                 aTTGAAGACCGTTTTCAGTTGCAGGGTGATCA‑GGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGa                         <  1:284604/151‑1 (MQ=255)                                                                                                                   tGAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGAc                        >  1:240595/1‑151 (MQ=255)                                                                                                                    gAAGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGAcg                       >  1:330049/1‑151 (MQ=255)                                                                                                                     gaGACCGTTTTCAGTTGCGGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGAcgc                      <  1:311388/150‑1 (MQ=255)                                                                                                                      aGACCGTTTTCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGAcgcg                     >  1:273533/1‑151 (MQ=255)                                                                                                                            ttttCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGaaaaa                >  1:53790/1‑150 (MQ=255)                                                                                                                               tCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATc             <  1:204866/150‑1 (MQ=255)                                                                                                                               tCAGTTGCAGGGTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATCGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCg            >  1:411956/1‑151 (MQ=255)                                                                                                                                        gggTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCa    >  2:127122/1‑150 (MQ=255)                                                                                                                                         ggTGATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCaaa  <  1:577328/151‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                     TGCTGATGGGGTGAACTCCATCCTTGCCGAAAAATTGGGGATGGCAAAACGCGTCAAACCGACGGATGTGGCGGTTGGCGTGAAGGAACTGATCGAGTTACCGAAGTCGGTTATTGAAGACCGTTTTCAGTTGCAGGGTAATCAGGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGA‑TGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAA  >  NC_000913/44653‑44939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑