Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	798,120	A→G	G104G (GGT→GGC)	ybhA ←	pyridoxal phosphate (PLP) phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	798,120	0	A	G	100.0%	148.2 / NA	40	G104G (GGT→GGC)	ybhA	pyridoxal phosphate (PLP) phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/22);  total (18/22)

CGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAGCAGGTGTATCCAGCGCCAGCGCC  >  NC_000913/797972‑798267                                                                                                                                                     |                                                                                                                                                    cGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGcc                                                                                                                                                   <  1:557656/151‑1 (MQ=255)    tAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTg                                                                                                                                                >  1:645456/1‑151 (MQ=255)      aCTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGaa                                                                                                                                              <  1:323193/151‑1 (MQ=255)            tgCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATgtggt                                                                                                                                        >  2:207575/1‑151 (MQ=255)             gcgcCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGGGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATgtggtg                                                                                                                                       >  2:18708/1‑151 (MQ=255)                  gTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGttcat                                                                                                                                  <  1:321197/151‑1 (MQ=255)                         ccAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCAt                                                                                                                           >  2:124091/1‑151 (MQ=255)                         ccAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCAt                                                                                                                           <  1:642830/151‑1 (MQ=255)                            gagaAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCAtctc                                                                                                                        <  1:323139/151‑1 (MQ=255)                             agaAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCa                                                                                                                       <  1:218442/151‑1 (MQ=255)                                              aaGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCttt                                                                                                      >  2:423209/1‑151 (MQ=255)                                                gTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTtaa                                                                                                    <  2:113042/151‑1 (MQ=255)                                                  cGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTtaata                                                                                                  <  2:524059/151‑1 (MQ=255)                                                   ggACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTtaata                                                                                                  >  2:130351/1‑150 (MQ=255)                                                   ggACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTtaata                                                                                                  >  1:76899/1‑150 (MQ=255)                                                      cGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACgg                                                                                              >  1:25341/1‑151 (MQ=255)                                                       gCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGCGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACggg                                                                                             >  2:177945/1‑151 (MQ=255)                                                                 cggcAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTcc                                                                                   <  1:529379/151‑1 (MQ=255)                                                                      gggTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTc                                                                              <  1:27046/151‑1 (MQ=255)                                                                            gcgcCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCAc                                                                        <  2:518294/151‑1 (MQ=255)                                                                                     tAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGc                                                               >  2:482856/1‑151 (MQ=255)                                                                                                  gACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATAc                                                  >  2:328688/1‑151 (MQ=255)                                                                                                   aCATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATAc                                                  >  1:244563/1‑150 (MQ=255)                                                                                                     aTGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACaaa                                               <  2:230729/151‑1 (MQ=255)                                                                                                       gCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATa                                             <  2:81676/151‑1 (MQ=255)                                                                                                        cccGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAAt                                              >  2:119972/1‑149 (MQ=255)                                                                                                                     tCATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGc                               >  2:177273/1‑151 (MQ=255)                                                                                                                      cATAGACCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCa                              <  2:97625/151‑1 (MQ=255)                                                                                                                           aCCATTGCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAg                         <  2:1737/151‑1 (MQ=255)                                                                                                                                 gCATCATCGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAGCAGgtg                   <  1:320304/151‑1 (MQ=255)                                                                                                                                     catcGACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAGCAGGTGTAt                <  2:132516/150‑1 (MQ=255)                                                                                                                                         gACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAGCAGGTGTATccagc           <  2:178817/151‑1 (MQ=255)                                                                                                                                         gACATACATCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAGCAGGTGTATccagc           >  2:578249/1‑151 (MQ=255)                                                                                                                                            atacatCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAGCAGGTGTATccagcgcc        >  2:39793/1‑151 (MQ=255)                                                                                                                                              acatCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAGCAGGTGTATccagcgcca       <  2:593088/150‑1 (MQ=255)                                                                                                                                              acaacaGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAGCAGGTGTATccagcgcc        >  1:317203/1‑149 (MQ=255)                                                                                                                                               catCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAGCAGGTGTATccagcgccagc     <  1:10023/151‑1 (MQ=255)                                                                                                                                               catCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAGCAGGTGTATccagcgccagc     >  2:635273/1‑151 (MQ=255)                                                                                                                                               catCAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAGCAGGTGTATccagcgccagc     <  1:612409/151‑1 (MQ=255)                                                                                                                                                  cAGGCCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAGCAGGTGTATCCagcgccagcgcc  <  2:102511/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CGTTAACTTGTTGCGCCGTTTCAGCCAGAGAAGCGACTTGTGTGAAAGTCGGACGCTGTTCCGGCGGCAGGGTTTGCGCCCAGTTAGATGTGCGAATGACATGCCCGGTCGGGTGCTCATAGACCATTGCATCATCGACATACATCAGACCGTGAATGTGGTGTTCATTCAGCATCTCAATGAGTTGCAGGGCTTTAATAACGGGCATTGGGTCCGCTTCCAGCACGGTTTTTGCATGATAATCATACAAATAGGTGCCATTACAGCAAATAGCAGGTGTATCCAGCGCCAGCGCC  >  NC_000913/797972‑798267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑