Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	817,261	A→G	E73G (GAG→GGG)	moaA →	molybdopterin biosynthesis protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	817,261	0	A	G	100.0%	118.5 / NA	35	E73G (GAG→GGG)	moaA	molybdopterin biosynthesis protein A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/16);  total (19/16)

AACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCATT  >  NC_000913/817116‑817403                                                                                                                                                  |                                                                                                                                               aaCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGt                                                                                                                                           <  1:2086/151‑1 (MQ=255)  aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTc                                                                                                                                          <  2:575623/151‑1 (MQ=255)     ttCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCtt                                                                                                                                        <  2:463016/150‑1 (MQ=255)             aCCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCcgcg                                                                                                                               >  1:290613/1‑151 (MQ=255)                tACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACt                                                                                                                            >  1:306814/1‑151 (MQ=255)                       tgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCg                                                                                                                      >  2:276889/1‑150 (MQ=255)                           ggATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATatc                                                                                                                 <  1:655254/151‑1 (MQ=255)                             aTGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATatcat                                                                                                               >  2:599388/1‑151 (MQ=255)                                   aCAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGTTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATcgcc                                                                                                           <  2:8315/149‑1 (MQ=255)                                   aCAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATcgccgc                                                                                                         <  2:283912/151‑1 (MQ=255)                                    cAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCt                                                                                                        >  1:414422/1‑151 (MQ=255)                                       aCCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCtgtg                                                                                                     <  1:6558/151‑1 (MQ=255)                                         cGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCg                                                                                                   <  1:205543/151‑1 (MQ=255)                                           aGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCggg                                                                                                 >  2:677596/1‑151 (MQ=255)                                             cggcgTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGa                                                                                                >  2:437280/1‑150 (MQ=255)                                             cggcgTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGa                                                                                                >  1:496466/1‑150 (MQ=255)                                                  tCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAac                                                                                            >  2:484558/1‑149 (MQ=255)                                                    aCCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAacgacg                                                                                        >  2:392373/1‑151 (MQ=255)                                                    aCCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAacgacg                                                                                        <  2:125049/151‑1 (MQ=255)                                                          aaaGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATcc                                                                                  <  1:152080/151‑1 (MQ=255)                                                           aaGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCg                                                                                 >  2:215716/1‑151 (MQ=255)                                                                tttCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGcc                                                                               >  2:181770/1‑148 (MQ=255)                                                                                   ttCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTAt                                                                                    >  2:299206/1‑124 (MQ=255)                                                                                   ttCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTAt                                                                                    <  1:299213/124‑1 (MQ=255)                                                                                   ttCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATgg                                                         >  2:69973/1‑151 (MQ=255)                                                                                        cGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTAcc                                                    >  1:535210/1‑151 (MQ=255)                                                                                           gTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTc                                                 <  2:129757/151‑1 (MQ=255)                                                                                             tACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTg                                               >  1:143295/1‑151 (MQ=255)                                                                                                     ccTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGa                                       <  1:177599/151‑1 (MQ=255)                                                                                                      cTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAAcgc                                         <  1:141329/148‑1 (MQ=255)                                                                                                       ttCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGAtg                                     >  1:206621/1‑151 (MQ=255)                                                                                                           ccAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGc                                 >  1:74245/1‑151 (MQ=255)                                                                                                                      aCCGAAAAAGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGgcgc                       <  2:551467/150‑1 (MQ=255)                                                                                                                           aaaaGTGCGCCTGACAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCg                 <  1:294606/151‑1 (MQ=255)                                                                                                                                         cAGGAGGAGGGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCAt   >  1:47485/1‑151 (MQ=255)                                                                                                                                          aggaggaggGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCAtt  >  2:385480/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                               AACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCCAGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGACTTTACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACCAATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCATT  >  NC_000913/817116‑817403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑