Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	841,490	C→T	S14S (TCG→TCA)	fiu ←	catecholate siderophore receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	841,490	0	C	T	100.0%	194.9 / NA	51	S14S (TCG→TCA)	fiu	catecholate siderophore receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/31);  total (20/31)

TTCGGATCGGCAGATTGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGA  >  NC_000913/841344‑841637                                                                                                                                                   |                                                                                                                                                    ttCGGATCGGCAGATTGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGaa                                                                                                                                                  <  2:191439/150‑1 (MQ=255)   cGGATCGGCAGATTGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGa                                                                                                                                               <  1:329015/151‑1 (MQ=255)   cGGATCGGCAGATTGTTGTGGCGCATAAAGCGAAGGCGTTGAGGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATg                                                                                                                                                <  2:314786/150‑1 (MQ=255)     gATCGGCAGATTGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGaaa                                                                                                                                             <  2:10598/151‑1 (MQ=255)     gATCGGCAGATTGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGaaa                                                                                                                                             <  1:659785/151‑1 (MQ=255)      aTCGGCAGATTGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAAt                                                                                                                                            <  1:587705/151‑1 (MQ=255)                tgttgtGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCa                                                                                                                                   <  1:394654/150‑1 (MQ=255)                   tgtgGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAggg                                                                                                                                >  2:466667/1‑150 (MQ=255)                     tgGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGaa                                                                                                                              >  1:152398/1‑150 (MQ=255)                        cgcATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTg                                                                                                                          <  2:438915/151‑1 (MQ=255)                           aTAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCg                                                                                                                        >  1:430214/1‑150 (MQ=255)                            tAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGAt                                                                                                                      >  1:247406/1‑151 (MQ=255)                             aaaGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGAtt                                                                                                                     >  2:241972/1‑151 (MQ=255)                               aGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGt                                                                                                                   <  2:409921/151‑1 (MQ=255)                               aGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGt                                                                                                                   <  1:336081/151‑1 (MQ=255)                                gCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGtt                                                                                                                  >  1:526710/1‑151 (MQ=255)                                    aGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTcc                                                                                                              <  1:546050/151‑1 (MQ=255)                                     ggCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCa                                                                                                             <  2:219279/151‑1 (MQ=255)                                     ggCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTCAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCa                                                                                                             <  2:283096/151‑1 (MQ=255)                                      gCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCAt                                                                                                            >  1:26553/1‑151 (MQ=255)                                       cGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCAtt                                                                                                           <  1:339909/151‑1 (MQ=255)                                       cGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCAtt                                                                                                           >  1:213970/1‑151 (MQ=255)                                              gCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAgg                                                                                                    <  2:555457/151‑1 (MQ=255)                                                ttCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTg                                                                                                  >  2:323170/1‑151 (MQ=255)                                                   gACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACt                                                                                               <  1:11124/151‑1 (MQ=255)                                                       aCCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACttttt                                                                                           <  1:116498/151‑1 (MQ=255)                                                         cAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCt                                                                                         >  2:291440/1‑151 (MQ=255)                                                          aGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCtt                                                                                        >  2:158020/1‑151 (MQ=255)                                                          aGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCtt                                                                                        >  1:419499/1‑151 (MQ=255)                                                            cGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTtat                                                                                      <  1:70409/151‑1 (MQ=255)                                                                  aTCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTg                                                                                <  1:563565/151‑1 (MQ=255)                                                                                 cccTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCa                                                                 <  2:278985/151‑1 (MQ=255)                                                                                       ggcggcGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACgaga                                                            >  1:412905/1‑150 (MQ=255)                                                                                                     gAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCgg                                              <  2:234589/150‑1 (MQ=255)                                                                                                         cacaGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGgatgcg                                         <  2:656239/151‑1 (MQ=255)                                                                                                            aGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGgatgcgatg                                      <  1:156206/151‑1 (MQ=255)                                                                                                              gCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGcc                                    <  1:379851/151‑1 (MQ=255)                                                                                                              gCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGcc                                    <  1:134521/151‑1 (MQ=255)                                                                                                               cGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGcc                                    >  2:10625/1‑150 (MQ=255)                                                                                                                      ccAATACAAAGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTa                             >  1:28232/1‑150 (MQ=255)                                                                                                                             aaaGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTg                     >  1:7393/1‑151 (MQ=255)                                                                                                                              aaGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGa                    <  2:26553/151‑1 (MQ=255)                                                                                                                               aGACCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGAt                   <  2:79397/151‑1 (MQ=255)                                                                                                                                 aCCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGAt                   >  2:596625/1‑149 (MQ=255)                                                                                                                                 aCCGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAg                 <  2:151056/151‑1 (MQ=255)                                                                                                                                   cGGCAAAGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTg               >  2:357642/1‑151 (MQ=255)                                                                                                                                       aaaGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCg           >  2:445347/1‑151 (MQ=255)                                                                                                                                       aaaGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCg           <  2:535819/151‑1 (MQ=255)                                                                                                                                        aaGAACGTGAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGt          <  2:152393/151‑1 (MQ=255)                                                                                                                                                gAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTg   >  2:440034/1‑150 (MQ=255)                                                                                                                                                gAGTGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGa  <  1:598480/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TTCGGATCGGCAGATTGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGA  >  NC_000913/841344‑841637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑