Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	845,744	A→G	S240S (TCT→TCC)	glnQ ←	glutamine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	845,744	0	A	G	100.0%	151.4 / NA	39	S240S (TCT→TCC)	glnQ	glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/24);  total (15/24)

TGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTC  >  NC_000913/845610‑845888                                                                                                                                       |                                                                                                                                                  tGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCaaa                                                                                                                                    >  2:349920/1‑150 (MQ=255)   aCAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAAt                                                                                                                                 <  1:626705/151‑1 (MQ=255)          gCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGc                                                                                                                           <  2:330361/150‑1 (MQ=255)          gCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCa                                                                                                                          <  2:24021/151‑1 (MQ=255)             ggCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAgc                                                                                                                       >  2:378099/1‑151 (MQ=255)                  ttttGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTg                                                                                                                   <  1:631817/150‑1 (MQ=255)                      gCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTc                                                                                                               <  1:224195/150‑1 (MQ=255)                           aTGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCggg                                                                                                         >  1:61388/1‑151 (MQ=255)                             gcggcgTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCggggt                                                                                                       >  1:574588/1‑149 (MQ=255)                             gcggcgTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGt                                                                                                        <  2:85835/150‑1 (MQ=255)                               ggcgTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCt                                                                                                     <  2:415534/151‑1 (MQ=255)                                      aCGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCaa                                                                                              <  2:253566/151‑1 (MQ=255)                                      aCGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCa                                                                                               >  1:605482/1‑150 (MQ=255)                                             aTCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTg                                                                                       <  1:332700/150‑1 (MQ=255)                                                cGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCgg                                                                                    >  1:561156/1‑150 (MQ=255)                                                  tCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGAtt                                                                                 >  2:71375/1‑151 (MQ=255)                                                        aaGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATc                                                                           <  2:462526/151‑1 (MQ=255)                                                        aaGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATc                                                                           <  1:238338/151‑1 (MQ=255)                                                                 cAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTTTTGGTCAA‑CACCTGCGGATTGCCATCTTCCGCaa                                                                   >  1:223852/1‑150 (MQ=255)                                                                  aaTTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATc                                                                 <  1:151085/151‑1 (MQ=255)                                                                     tttAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCCTCGGATTGCCATCTTCCGCCATCCgg                                                              >  2:303195/1‑151 (MQ=255)                                                                           aaGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCttt                                                         <  2:451626/150‑1 (MQ=255)                                                                           aaGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCttt                                                         >  1:521258/1‑150 (MQ=255)                                                                                     atatCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGa                                              <  1:41917/151‑1 (MQ=255)                                                                                      tatCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCCGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATaaa                                                >  2:351632/1‑148 (MQ=255)                                                                                       atCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGAt                                             <  1:482243/150‑1 (MQ=255)                                                                                       atCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGAt                                             <  1:279301/150‑1 (MQ=255)                                                                                         ccTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCa                                           <  2:76460/150‑1 (MQ=255)                                                                                           tGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGGTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGcc                                        >  2:491267/1‑151 (MQ=255)                                                                                             tAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGGTCCTGGTCAACCACCT‑CGGGTTGACCTCCTCCGCCATCCGGCCTTTGTCGATAAAGATCAGcccc                                      >  2:453170/1‑149 (MQ=255)                                                                                                gCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGaa                                   <  2:224936/151‑1 (MQ=255)                                                                                                gCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGACTTTGTCGATAAAGATCAGCCGCGaa                                   <  2:421348/151‑1 (MQ=255)                                                                                                  cTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTTTCGATAAAGATCAGcc                                        >  2:258863/1‑144 (MQ=255)                                                                                                       aaGCGTAGCGCATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CAACTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACtt                            >  1:393773/1‑151 (MQ=255)                                                                                                                 cATCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCaaa                  <  2:216334/151‑1 (MQ=255)                                                                                                                  aTCAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCaaaa                 <  2:32950/151‑1 (MQ=255)                                                                                                                    cAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAAcc               <  1:71377/151‑1 (MQ=255)                                                                                                                    cAGGCAATGTGTCTTATTAGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAAc                <  1:654944/150‑1 (MQ=255)                                                                                                                                  tattaGGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTc  <  2:220924/150‑1 (MQ=255)                                                                                                                                       |                                                                                                                                                  TGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTGTCTTATTAAGAGACGTGCTGCAAAAATTCCTGCAAGCGCTGGCTCGGCGGGTTCTTGATCAA‑CACCTGCGGATTGCCATCTTCCGCAATCCGGCCTTTGTCGATAAAGATCAGCCGCGAAGCTACTTTCTCGGCAAAACCGATTTCGTGGGTC  >  NC_000913/845610‑845888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑