Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	134,458	T→C	V24A (GTC→GCC)	yacL →	UPF0231 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	134,458	0	T	C	100.0%	178.0 / NA	51	V24A (GTC→GCC)	yacL	UPF0231 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/27);  total (24/27)

CCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGAT  >  NC_000913/134314‑134605                                                                                                                                                 |                                                                                                                                                    ccGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCgg                                                                                                                                                  <  1:401134/148‑1 (MQ=255) ccGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCa                                                                                                                                               <  1:356068/151‑1 (MQ=255)    gTAAGGCTGCGATAAGTACATCAATGGCGCAAGGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTTCGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGGGTATGTCCATGGGGCATGAAGTGGCCGGGCACTg                                                                                                                                            <  2:84029/151‑1 (MQ=255)      acggCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGt                                                                                                                                          >  1:332917/3‑151 (MQ=255)         gCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGttt                                                                                                                                        >  1:92056/1‑150 (MQ=255)                 aaTTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAagag                                                                                                                               <  1:404382/151‑1 (MQ=255)                     aCATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTga                                                                                                                           <  2:290361/151‑1 (MQ=255)                              gcgcAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAAcc                                                                                                                  <  1:178887/151‑1 (MQ=255)                                 cAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTgg                                                                                                               >  2:554099/1‑151 (MQ=255)                                     gCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCctt                                                                                                           >  1:459343/1‑151 (MQ=255)                                      cGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttg                                                                                                          <  1:282479/151‑1 (MQ=255)                                       gATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttgc                                                                                                         >  2:449546/1‑151 (MQ=255)                                            cGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTtgat                                                                                                    >  1:121628/1‑151 (MQ=255)                                            cGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTtgat                                                                                                    <  2:102736/151‑1 (MQ=255)                                            cGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTtgat                                                                                                    >  1:674059/1‑151 (MQ=255)                                            cGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTtga                                                                                                     <  2:587014/150‑1 (MQ=255)                                                     gCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGa                                                                                            <  2:101081/150‑1 (MQ=255)                                                        gggAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACa                                                                                         >  2:23155/1‑150 (MQ=255)                                                           aGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAagca                                                                                     <  1:35854/151‑1 (MQ=255)                                                                  gAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTTGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGcgcac                                                                               <  2:103290/150‑1 (MQ=255)                                                                  gAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGcgcacg                                                                              >  1:373100/1‑151 (MQ=255)                                                                   aaCACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGcgcacgc                                                                             <  1:178235/151‑1 (MQ=255)                                                                       cacTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTg                                                                         <  2:537216/151‑1 (MQ=255)                                                                       cacTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAACGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTg                                                                         <  2:252596/151‑1 (MQ=255)                                                                           aTGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGaaa                                                                      <  2:323065/150‑1 (MQ=255)                                                                           aTGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAg                                                                     <  2:288414/151‑1 (MQ=255)                                                                              gATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGt                                                                   >  1:2826/1‑150 (MQ=255)                                                                                   cGAATTGCTGCGCGACATTGCCGGGGTGGTAAAGGCGCGTATGCCCCTTGGGCATGAAGAGGCCGGGCACTGGATTAATGAAGAGGTGAAAGAAAAACTGGCCTTGCTTGATGAAGTGCAACAAGCAGCGCACGCACGGAAAGGTAGCGa                                                              >  2:57916/1‑150 (MQ=255)                                                                                    gAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAAc                                                            <  1:458126/151‑1 (MQ=255)                                                                                                 atatTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACg                                               >  2:37734/1‑151 (MQ=255)                                                                                                     tACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggc                                            >  1:18434/1‑150 (MQ=255)                                                                                                      aCCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggc                                            >  2:364344/1‑149 (MQ=255)                                                                                                        cGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcaggg                                        >  1:511330/1‑151 (MQ=255)                                                                                                         ggAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcagggc                                       >  2:559056/1‑151 (MQ=255)                                                                                                           aGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCAt                                     >  1:328900/1‑151 (MQ=255)                                                                                                             tGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATg                                    >  2:644398/1‑150 (MQ=255)                                                                                                                tAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAAt                                 >  2:354860/1‑150 (MQ=255)                                                                                                                        cGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCtgt                        <  2:495989/151‑1 (MQ=255)                                                                                                                         gTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCtgtg                       >  1:470477/1‑151 (MQ=255)                                                                                                                           aTGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtgga                     <  1:439492/151‑1 (MQ=255)                                                                                                                           aTGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtgg                      <  2:231338/150‑1 (MQ=255)                                                                                                                            tGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggat                    >  2:510048/1‑151 (MQ=255)                                                                                                                             gTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggat                    <  1:425870/150‑1 (MQ=255)                                                                                                                             gTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGACCTGGCAACGGACAGGGCATGAATACACCCTGtggat                    <  1:391084/150‑1 (MQ=255)                                                                                                                               ccATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatgga                 <  1:448641/151‑1 (MQ=255)                                                                                                                               ccATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatgg                  >  2:371580/1‑150 (MQ=255)                                                                                                                                cATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGAc                <  2:486730/151‑1 (MQ=255)                                                                                                                                      gCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGaa          <  1:114067/151‑1 (MQ=255)                                                                                                                                            aGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTg    <  2:187582/151‑1 (MQ=255)                                                                                                                                            aGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTg    >  1:638013/1‑151 (MQ=255)                                                                                                                                              tGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGAt  >  1:111015/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                    CCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGAT  >  NC_000913/134314‑134605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑